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Quick and high-efficiency cephalotaxus hainanensis seed seedling breeding method

A technology for seeds and torreya, applied in the field of fast and efficient breeding of torreya seeds in Hainan, can solve problems such as inability to apply and popularize, low seedling rate, and inability to emerge, and achieve the effect of rapid and efficient seedling raising

Inactive Publication Date: 2010-02-03
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are also people who have studied the use of traditional tissue culture methods to propagate seedlings of Torreya hainanensis, but basically no seedlings can emerge, or the emergence rate is very low, and application and promotion cannot be carried out (Fu Wenying, Du Daolin, Fu Mujun. Induction of callus of Torreya hainanensis and Cultivation. Communications of Plant Physiology, 2004, 40(1): 34-36; Li Zhiying, Wang Zhunian, Xu Li. Rapid Propagation of Torreya hainanensis in vitro. Communications of Plant Physiology, 2005, 41(5): 786)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Seed collection: collect 20 seeds of Torreya hainanensis that are close to maturity or mature;

[0015] (2) Cleaning and sterilization of seeds: Rinse the seeds of Torreya hainanensis with water for 1h., and clean them with distilled water. After blotted dry by filter paper, put them into 0.5% sodium hypochlorite solution for vacuum filtration and sterilization for 8 minutes, and then rinse them repeatedly with sterile water. 3 times;

[0016] (3) Seed low temperature release dormancy: put the sterilized Torreya hainanensis seeds at a low temperature of about 1°C for 1 week to release the dormancy of the seeds;

[0017] (4) Seed germination: remove the dormant seeds and then put them into 0.5% sodium hypochlorite solution for vacuum filtration sterilization for 3 minutes, then repeatedly rinse with sterile water for 3 times; then inoculate the seeds on the following medium for cultivation: 0.5MS (Murashige and SkoogStock medium)+0.01mg L -1 6-BA (6-benzylpurine) ...

Embodiment 2

[0020] (1) Seed collection: collect 20 seeds of Torreya hainanensis that are close to maturity or mature;

[0021] (2) Cleaning and sterilization of seeds: flush the seeds of Torreya hainanensis with water for 3 hours, and clean them with distilled water. After the filter paper is blotted dry, put them into 2% sodium hypochlorite solution for vacuum filtration and sterilization for 3 minutes, and then rinse them repeatedly with sterile water for 6 minutes. Second-rate;

[0022] (3) Seed low temperature release dormancy: put the sterilized Torreya hainanensis seeds at a low temperature of about 6°C for 2 weeks to release the dormancy of the seeds;

[0023] (4) Seed germination: remove the dormant seeds and then put them into 2% sodium hypochlorite solution for vacuum filtration sterilization for 8 minutes, then repeatedly rinse with sterile water for 6 times; then inoculate the seeds on the following medium for cultivation: 1MS (Murashige and SkoogStock culture medium)+0.1mg·L...

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Abstract

The invention provides a quick and high-efficiency cephalotaxus hainanensis seed seedling breeding method which belongs to a quick and high-efficiency plant seed seedling breeding method. The method comprises the following steps: (1) seed collection; (2) cleaning and sterilization of seeds; (3) release of seed dormancy at low temperature; (4) sterile germination of seeds; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud rate to more than 90% (enhanced by about 70%), and the seeding viability exceeds 90% (enhanced by about 60%); compared with the traditional method, the stocking time is shortened by about 2 / 3; the regular high-quality cephalotaxus breeding seedlings can be produced; and the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and utilization of resources.

Description

technical field [0001] The invention relates to a rapid and efficient breeding method for seeds and seedlings of plants, in particular to a rapid and efficient breeding method for Hainan rough torreya seeds and seedlings. Background technique [0002] Hainan rough torreya (Cephalotaxus hainanensis Li) is a plant of the family Cephalotaxus, aliased as Red Cattle Pine and Thin-leaved Castor Cedar, and is a perennial tall evergreen tree. It is a rare tree species unique to my country, and its distribution range is extremely narrow, only found in Hainan, Guangdong, Yunnan and other local areas. Studies have shown that not only the effective alkaloid content of Torreya hainanensis is the highest, but also the curative effect is the most significant. It is currently recognized as one of the best anti-cancer tree species. In addition, this tree species has been felled repeatedly due to its excellent material, and its population is small. It is one of the most important rare and en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G9/10A01C1/08A01C1/00A01G31/00
Inventor 杜道林祁珊珊林英宋经元杨冬华王有生魏建和
Owner JIANGSU UNIV
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