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An efficient sterile sowing and seedling raising method for hybrid seeds of camellia nitidissima Chi

A hybrid seed and aseptic sowing technology, applied in the field of plant seedling raising, can solve the problems of hybrid germplasm loss and low seedling emergence rate of golden camellia tea, and achieve the effects of shortening the seeding and seedling time, promoting the germination of many seedlings, and improving the seedling raising and sowing efficiency.

Active Publication Date: 2017-09-26
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the emergence rate of hybrid seedlings of Camellia japonica is extremely low, about 7.5%, and the hybrid germplasm of Camellia japonica is seriously lost.

Method used

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  • An efficient sterile sowing and seedling raising method for hybrid seeds of camellia nitidissima Chi

Examples

Experimental program
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Embodiment 1

[0032] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:

[0033] Step 1: Select hybrid seeds with plump seeds, no diseases and insect pests and no trauma (seeds of the hybrid species of Camellia japonica♀ and Camellia ‘Liexiang’♂);

[0034] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 50 seconds, then rinse twice with sterile water, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.2% for 4 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;

[0035] Step 3: Pack the washed and drained fine sand into culture bottles. The thickness of each bottle of fine sand is about 2cm. Seal the culture bottle and place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the con...

Embodiment 2

[0040] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:

[0041] Step 1: Select hybrid seeds with plump seeds, no pests and injuries (seeds of the hybrid species of Camellia japonica♀ and Camellia japonica♂ in Fangcheng);

[0042] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 70 seconds, then rinse with sterile water for 4 times, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.6% for 5 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;

[0043] Step 3: Pack the washed and drained fine sand into culture bottles. The thickness of each bottle of fine sand is about 2cm. Seal the culture bottle and place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the conditi...

Embodiment 3

[0048] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:

[0049] Step 1: Select hybrid seeds with plump seeds, no pests and injuries (seeds of hybrids of Fangcheng Camellia ♀ and Vietnamese Muscle Tea ♂);

[0050] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 60 seconds, then rinse with sterile water for 3 times, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.4% for 5 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;

[0051] Step 3: Pack the washed and drained fine sand into culture bottles, the thickness of each bottle of fine sand is about 2cm, seal the culture bottle, and then place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the condition of 30min...

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Abstract

An efficient sterile sowing and seedling raising method for hybrid seeds of camellia nitidissima Chi is provided. The method includes selecting the hybrid seeds free of disease and pest harms and free of injuries; disinfecting the seeds to obtain the sterile hybrid seeds; sterilizing a sowing medium; then under sterile conditions, sowing the disinfected hybrid seeds to sterilized fine sand or fine sand to which 6-benzylamino adenine is added; after sowing, culturing the hybrid seeds at 23-25 DEG C under complete-dark conditions until cotyledons are exposed from the sowing medium; then culturing the seedlings at 23-25 DEG C under a condition that illumination intensity of natural scattered light is 2800-32001x until the heights of the seedlings are 4-5 cm; and then transplanting the seedlings to a transplanting medium in a sealed environment the air humidity of which is 80-90% and the temperature of which is 23-25 DEG C. The method can increase the germination rate of the hybrid seeds of camellia nitidissima Chi, can increase the sowing and seedling raising efficiency and can reduce loss of hybrid germplasm.

Description

technical field [0001] The invention relates to the technical field of plant seedling cultivation, in particular to an efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds. Background technique [0002] Camellia chrysantha(Hu)Tuyama is the only rare ornamental plant with golden yellow flowers in the Camellia family Theaceae. Queen". The fruit setting rate of camellia camellia distant hybridization is as low as 1.5%, and some embryos of the hybrids are deformed or stunted. Therefore, the hybrid seeds of camellia camellia are very precious. However, the emergence rate of hybrid seeds of Camellia japonica by conventional sowing and raising seedlings is extremely low, about 7.5%, and the hybrid germplasm of Camellia japonica is seriously lost. Contents of the invention [0003] The object of the present invention is to overcome above-mentioned defect, provide a kind of method for aseptic sowing and seedling raising of golden camellia...

Claims

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Application Information

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IPC IPC(8): A01C1/08A01C1/00A01G31/00
CPCA01C1/00A01C1/08A01G31/00
Inventor 吴丽君高楠陈达
Owner FUJIAN ACAD OF FORESTRY
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