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Aseptic seedling mycorrhizal method for paphiopedilum armeniacum

A technology of Paphiopedilum apricot and aseptic seedlings, which is applied in the field of mycorrhizalization of Paphiopedilum apricot aseptic seedlings, which can solve the problems of slow growth and difficulty in flowering of aseptic seedlings

Inactive Publication Date: 2009-04-08
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology solves the problem of slow growth and difficulty in flowering in aseptic seedling cultivation, and this method is simple to operate, has obvious effects, and has strong ecological and social benefits

Method used

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  • Aseptic seedling mycorrhizal method for paphiopedilum armeniacum
  • Aseptic seedling mycorrhizal method for paphiopedilum armeniacum

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Embodiment 1

[0017] The implementation route of mycorrhization of Paphiopedilum apricot aseptic vaccine:

[0018] 1. Preparation of aseptic seedlings of Paphiopedilum apricot: Aseptic seedlings of Paphiopedilum apricot are derived from aseptic germination of seeds, and it takes about 2 years from sowing to out of the bottle.

[0019] 2. Preparation of adult plants containing Glycolemma mesorhiza in mycorrhizae: using the method of amplifying ITS fragments of mycorrhizal fungi in roots to select adult plants of P. apricotii P.

[0020] 3. Cultivation substrate preparation: first use the ratio of 2: 1: 1 (volume ratio) stone, bark and humus to form the substrate cultivation of the above-mentioned selected P. apricotii adult plant for no less than six months, so that Bacteria-carrying culture medium. Use the same amount or more (no more than twice the old substrate) new humus and bark to mix with the fungus-carrying substrate that has cultivated adult plants. The substrate of the cultivated...

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Abstract

Paphiopedilum armeniacum, known as 'Golden Child', is one of orchidaceous endangered species in China and has extremely high aesthetic values. Successful and perfect artificial propagation technology has significant importance to the protection and development of paphiopedilum armeniacum. The invention discloses a method which is to introduce tulasnella c alospora into a substrate by using a grown paphiopedilum armeniacum plant and then cultivate aseptic seedlings of paphiopedilum armeniacum in the substrate carrying tulasnella c alospora so as to raise mycorrhizal seedlings of paphiopedilum armeniacum. The invention aims to provide a method for raising mycorrhizal seedlings of paphiopedilum armeniacum with low cost and easy operation to solve the problem of slow growth of cultivated aseptic seedlings. The method has the advantages of readily available tulasnella c alospora resource, easy operation of impregnation, low cost, etc., and has obvious economic and ecological efficiency.

Description

[0001] Affiliated field: the invention belongs to the field of biotechnology, in particular, it relates to a method for mycorrhizalization of Paphiopedilum apricot aseptic seedlings. Background technique: [0002] Paphiopedilum plants are one of the most ornamental groups of Orchidaceae. Although P. armeniacum is a species discovered later in the genus, it has been exhibited at international flower exhibitions and orchids many times. It is a species that has attracted international attention. Due to the extraordinary ornamental value of the original species of Paphiopedilum apricot, a large number of its wild plants have been excavated. At present, it is on the verge of extinction. It is urgent to take various protection measures to save this pearl among orchids. Carrying out research on artificial breeding technology can not only alleviate the pressure of its wild collection, but also has important significance for the protection of its species and the development of resource...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A01G31/00C12Q1/68C12Q1/04
Inventor 胡虹袁莉李树云黄家林严宁
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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