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Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense

A technology of bacilli and strains, applied in the direction of bacteria, applications, fungicides, etc., can solve the problems of molan production impact, serious damage, etc., and achieve the effects of small ecological environment impact, strong inhibition, and low requirements for cultivation conditions

Active Publication Date: 2013-01-23
中科生物技术转移(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Investigations in various cities and counties (districts) in Guangdong Province found that the disease is common in Molan planting bases, and the damage is serious. The incidence rate of some flower farms is as high as 30%, which has seriously affected the production of Molan.

Method used

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  • Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense
  • Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense
  • Bacterium paenibacillus polymyma SHL-1 and application thereof in preventing and controlling stalk rot of cymbidium sinense

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Paenibacillus polymyxa Inhibitory Activity of SHL-1 Culture Solution on Stem Rot Fungus

[0020] The SHL-1 strain was isolated from Dendrobium orchid leaves by conventional tissue isolation method. After the strain was cultured on LB medium for 48 h, the single colony was round, white, smooth, opaque, slightly raised, and the edges were neat and wrinkled. The bacterium was analyzed by 16S rDNA sequence, and its sequence was the same as that of GenBank with accession number HQ317186.1. Paenibacillus polymyxa The homology reaches 99%; the BIOLOG bacterial identification system software analysis shows that the SHL-1 strain is only related to Paenibacillus polymyxa in the database ( Paenibacillus polymyxa ) are well matched, with a Similarity (SIM) of 0.834, a Distance (DIS) of 2.48, and a Probability (PROB) of 99%.

[0021] The activity of the live bacterial liquid of the strain was determined by the zone of inhibition method. Bacterial strains were inocul...

Embodiment 2

[0022] Example 2 Paenibacillus polymyxa Antibacterial Spectrum of SHL-1 Culture Medium

[0023] Taking several flower fungal disease pathogens as objects, the inhibition zone method was used to determine the Paenibacillus polymyxa Antibacterial spectrum of the SHL-1 strain. Bacterial strains were inoculated in LB medium, and cultured at 28 °C and 200 r / min for 48 h with shaking. Use a puncher with an inner diameter of 0.6 mm to punch holes on the PDA medium, add 80 μL of bacterial solution into the holes, and inoculate P. Sclerotinia macrophylla and phalaenopsis peduncle rot bacteria block. With the addition of LB liquid medium as the control, each treatment was repeated 3 times, and cultured in a constant temperature incubator at 25 °C. When the diameter of the control colony reached 6 cm, the width of the inhibition zone was measured.

[0024] Table 1 Paenibacillus polymyxa Inhibitory Effects of SHL-1 Fermentation Broth on Five Kinds of Pathogenic Bacteria

[002...

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Abstract

The invention discloses bacterium paenibacillus polymyma SHL-1 and an application thereof in preventing and controlling stalk rot of cymbidium sinense. The bacterium paenibacillus polymyma SHL-1 with a preservation number of CCTCCM2011441 is preserved in the China Center for Type Culture Collection on December 2, 2011. In the invention, the bacterium paenibacillus polymyma SHL-1 is inoculated to an LB (Langmuir-Blodgett) liquid culture medium with pH of 7.0, and is cultured for 48 hours in a oscillation way at 200r / min at a temperature of 28 DEG C, so that a biological preparation can be prepared. The biological preparation has a strong inhibition effect to the stalk rot of cymbidium sinense, and has environmentally-friendly non-toxic sources and small influence to biological environment;and furthermore, the biological preparation has low requirements to the culture condition, has an inhibition effect to various pathogenic fungi, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the field of biological pesticides, in particular to a strain of bacteria Paenibacillus polymyxa SHL-1 and the biological agent prepared therefrom and its application in the prevention and treatment of the stem rot of Molanca officinalis. Background technique [0002] Cymbidium( Cymbidium. sinense ) has a long history of cultivation in my country, mainly distributed in Guangdong, Guangxi, Fujian, Zhejiang, Sichuan, Yunnan and other places. In the flower industry, Molan belongs to the main species of high-end potted flowers, with extremely high economic value and ornamental value. In recent years, with the expansion of the planting area of ​​Molan, various diseases and insect pests have also appeared continuously, which has become one of the limiting factors affecting the production of Molan. Molan stem rot ( Fusarium oxysporum ), also known as fusarium wilt, is an important disease in the production of molan. When the da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N63/02A01P3/00C12R1/01
Inventor 向梅梅施祖荣张云霞黄江华
Owner 中科生物技术转移(深圳)有限公司
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