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Cymbidium sinense tissue culture method

A technique of tissue culture and orchid, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high induction pollution rate, low induction success rate, uneven differentiation of seedlings, etc., and reduce the pollution rate. , Improve the differentiation rate and breed less bacteria

Inactive Publication Date: 2019-10-08
三明市农业科学研究院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a method for culturing molan tissue, the main purpose of which is to overcome the defects of high induction pollution rate, low induction success rate and uneven differentiated seedlings in the existing molan tissue culture technology, so as to become high-quality seedlings of molan Breeding provides technical support

Method used

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  • Cymbidium sinense tissue culture method
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  • Cymbidium sinense tissue culture method

Examples

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Embodiment 1

[0024] refer to figure 1 , figure 2 and image 3 . A kind of Molan tissue culture method is to take the lateral buds of Molan "National Fragrance Peony" as explant, comprising the following steps:

[0025] 1) Acquisition of lateral buds: After selecting 2 to 3-year-old Molan mother plants that are free from diseases and insect pests and grow well, ① shake off the old substrate at the roots of the mother plants; Dried pine bark: perlite = 4:1 mixed matrix; after planting, the reed head of the mother plant is exposed by 0.5-1cm; ③Move the planted mother plant to the indoor greenhouse for growth, and manage it by alternating dry and wet methods Water, water irrigation is carried out in the way of root irrigation, and when the lateral buds grow to a height of 5-8cm, it is ready for use; indoor greenhouse cultivation conditions: temperature is 18-28°C, light is 4500-6000lux, and light time is 16h / d;

[0026] 2) Pre-disinfection treatment: Dry the lateral buds with a size of 5-...

Embodiment 2

[0034] A kind of Molan tissue culture method is to take the lateral bud of Molan "white ink" as explant, comprising the following steps:

[0035] 1) Acquisition of lateral buds: After selecting 2 to 3-year-old Molan mother plants that are free from diseases and insect pests and grow well, ① shake off the old substrate at the roots of the mother plants; Dried pine bark: perlite = 4:1 mixed matrix; after planting, the reed head of the mother plant is exposed by 0.5-1cm; ③Move the planted mother plant to the indoor greenhouse for growth, and manage it by alternating dry and wet methods Water, water irrigation is carried out in the way of root irrigation, and when the lateral buds grow to a height of 7-10cm, it is ready for use; indoor greenhouse cultivation conditions: temperature is 18-28°C, light is 4500-6000lux, and light time is 16h / d;

[0036] 2) Pre-disinfection treatment: Dry the lateral buds with a size of 7-10 cm in step 1 2 to 3 days in advance, then cut the lateral bud...

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Abstract

A cymbidium sinense tissue culture method includes steps of side bud obtaining, disinfection, induced culture, multiplication culture, differentiation culture, rooting culture and seedling hardening and transplanting. The method has the advantages that cymbidium sinense tissue culture induced side bud explants are obtained in a greenhouse which is relatively stable and clean, and a method of drying before material getting and disinfection pretreatment by enabling materials to enter a sterile environment directly after material getting is adopted, so that lowering of contamination rate is facilitated; the side bud explants selected for cymbidium sinense tissue culture inducing are newly growing second-generation buds, thereby being slight in bacteria breeding, high in activity and conduciveto inducing of rhizome, so that inducing success rate is increased; a rhizome material of Phi0.32-0.38cm in thickness and 3.5-5.5cm in length obtained after cymbidium sinense tissue culture rhizome differentiation, selection and multiplication is high in differentiation ability, thereby being conductive to increasing differentiation rate and obtaining robust differentiated seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for culturing molan tissue. Background technique [0002] Cymbidium sinense, also known as Baosuilan, is a ground-growing orchid of the Orchidaceae (Orchidaceae) orchid genus (Cymbidium). It is native to Taiwan, southern Fujian, Guangdong, Guangxi and other places in China. It is one of the seven major categories of orchids in China. . The leaf shape of Molan is elegant and unique, the inflorescences are erect, the flowers are large, the flowers are elegant, and the petals are full of fragrance. It has the reputation of "gentleman among flowers". At the same time, the root of Molan has certain medicinal effects for relieving asthma, and its flowers can also extract essential oils to make some cosmetics. [0003] At present, the traditional breeding of orchid seedlings in the market is mainly carried out by excavating a large number of wild orchids and bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林辉锋周辉明莫智龙宋彩凤陈昌铭吴森源林发壮郭芸伟夏朝水康永武曹奕鸯姚凤琴钟琳珊陈伟婷江斌
Owner 三明市农业科学研究院
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