Cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker and identification method thereof

Abstract

The invention provides a cymbidium sinense 'labellum and petal sepalody' character-related functional molecular marker. The functional molecular marker is M4wt or M4m, the nucleotide sequence of the M4wt is shown in SEQ ID NO: 1, and the nucleotide sequence of the M4m is shown in SEQ ID NO: 2. By separating and comparing related gene sequences of multiple varieties of cymbidium sinense, a functional specificity site, which is different from other varieties of cymbidium sinense, in 'labellum and petal sepalody' varieties 'Lvyun' and 'Taiyanghua' is obtained. Based on difference of the site, three specific primers M4F1, M4F2 and M4R are designed. A primer pair of M4F1 and M4R and a primer pair of M4F2 and M4R are utilized to amplify the total DNA of the genome of the cymbidium sinense, then agarose gel electrophoresis detection is performed, and thus the 'labellum and petal sepalody' varieties can be quickly identified. The method provided by the invention has great value and meaning in identification and protection of germplasm resources of cymbidium sinense, molecular marker-assisted breeding and molecular design breeding.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and more specifically relates to a functional molecular marker and an identification method related to the trait of "lips and petals and sepals" of the orchid. Background technique [0002] As a famous traditional Chinese flower, national orchid generally refers to some ground-growing species of the genus Orchid native to my country, such as Cymbidium goeringii, Cymbidium tortisepalum, Cymbidium ensifolium, Cymbidium kanran and ink Lan (Cymbidium sinense) and so on (Chen Xinqi, 1988). The cultivation of Chinese orchids began in the Tang and Song Dynasties and flourished in the Ming and Qing Dynasties. Since the Ming Dynasty's "Nanzhong Youfanglu" put forward the viewpoint of "discussing flowers by petals", the theory of petal shape has gradually become the benchmark for people to select and appreciate Chinese orchids, and it has been used until now. [0003] The standard form of the C...

AI Technical Summary

Problems solved by technology

Since the growth cycle of Molan japonica needs several years from seed germination to flowering, the exotic flower varieties can only be identified with the help of auxiliary traits such as leaf shape, root system, and leaf beetle during the non-flowering period, which not only requires the appraiser to have extremely rich experience, Moreover, these traits are easily affected by the planting environment, which greatly affects the reliability of the identification results.

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