106results about How to "Improve the success rate of cultivation" patented technology

The invention provides a bladder cancer organoid culture medium and a culture method. According to the culture method, two different organoid culture mediums are adopted for culture, and the composition and proportion of the two organoid culture mediums are disclosed. According to the method and the culture medium, the culture success rate, the passage frequency, the growth speed and the growth form of the bladder cancer organoid can be improved, and the problems that in the prior art, the culture success rate is low, the passage frequency is small, the organoid grows less, the cell form is poor, and growth is slow of the bladder cancer organoid are solved.

The invention discloses a method for breeding, culturing and storing plant nematodes by using sweet potato calluses. The method is implemented through the following steps of: cleaning fresh sweet potatoes, performing surface sterilization, peeling, slicing, placing into a culture dish, and culturing in an incubator to obtain sweet potato calluses; inoculating sterilized plant nematodes onto the sweet potato calluses; placing into respective incubators of appropriate temperatures, and culturing and breeding in dark; and after culturing for a certain period of time, storing the plant nematodes and the sweet potato calluses at the temperature of 4-20 DEG C. Due to the adoption of the method, the aims of manually culturing and breeding a large quantity of plant nematodes in a simple way and storing plant nematode resources for a long time can be fulfilled.

The invention discloses a seed treatment method for increasing the germination rate and improving the disease resistance of soybean seeds. The seed treatment method comprises the specific steps of seed pretreatment, preparation of seed soaking liquid, seed soaking, disease resistance treatment and sterilization treatment. The seed pretreatment comprises the following steps: putting mature soybean seeds into acidic soaking liquid with the temperature of 20 to 30 DEG C for 50 to 60 minutes, taking out the seeds, putting the seeds on sand paper, rinsing the seeds with clean water after wax on the outer skins of the seeds fall off, screening the seeds, and removing flat particles; the preparation of the seed soaking liquid comprises the following steps: adding the following components in weight proportion into a common aqueous liquid: 20 to 24g / L ammonium nitrate, 0.4 to 0.6g / L sodium phthalate, 0.3 to 0.5g / L sodium humate, 2 to 4mg / L nutrition adding liquid and 0.04 to 0.06mg / L gibberellin, and uniformly mixing for later use. The seed soaking liquid is enriched with nutritional elements, so that the soybean seeds can get through the period of dormancy within shorter time, the speed that the soybean seeds are germinated into seedlings is increased, irradiation of infrared achieves a sterilization effect; the seeds are soaked in potassium permanganate or formalin, the disease resistance of the seeds is enhanced, so that the culture success rate of the soybeans is increased, and high germination rate is realized.

The invention belongs to the technical field of plant cultivation and specifically relates to a medium for cultivating orchids and a preparation method thereof. The medium for cultivating orchids is prepared from the following raw materials: chestnut thorn, chestnut leaves or oak leaves, peanut shells and / or pine tree bark, gravel pebbles, rainwater and toshin. The orchid cultivated by the medium provided by the invention has the characteristics of excellent growth condition, dark green leaf color, strong root system, straight flower stalk, bright flower color, fragrance and long flowering phase; the replacing period of an orchid flowerpot is prolonged to 3-4 years; the success rate of orchid cultivation is increased, so that the living quality and grade of people can be promoted; and the cultivation cost of high-class flowers, such as orchids, can be lowered, so that new power is supplied for the development of the flower career.

The invention discloses a cultivation method of Gastrodia elata Bl. and belongs to the technical field of Gastrodia elata Bl. planting. The method includes the steps of preparation of a halimasch culture medium, preparation of bacteria sticks, inoculation, Gastrodia elata Bl. cultivation and management. By controlling the process of cultivating halimasch and improving a Gastrodia elata Bl. cultivation method, the aim to improve the yield of Gastrodia elata Bl. is achieved, and the Gastrodia elata Bl. prepared by the cultivation method is uniform in size, full, and good in appearance and quality.

The invention provides a culture medium, a culture method and a detection method of a thyroid cancer organoid, the culture medium comprises a basic culture medium, B-27, N-acetylcysteine, nicotinamide, R-spondin 1 recombinant protein and other components, the culture medium has high thyroid cancer organoid culture success rate, high passage frequency, and good passage stability after repeated cryopreservation and resuscitation; the thyroid cancer organoid obtained by adopting the culture medium and the culture method is high in reducibility; and the detection method is comprehensive and accurate.

The invention provides a 3D culture system of primary tumor cells, and an application of the 3D culture system. The 3D culture system comprises lower layer gelatin, a middle layer cell substrate and an upper layer culture medium, wherein the middle layer cell substrate comprises improved agarose gel, a condition culture medium containing addition factors and tumor cells; the improved agarose gel comprises low melting-point agarose, polylysine and an extracellular matrix; the lower layer gelatin comprises agarose gel and a conditioned medium; and the upper layer culture medium is the conditionculture medium containing the addition factors. The 3D culture system provided by the invention can screen tumor cells and normal epithelial cells, besides, can realize preferential growth of the tumor cells, is high in culture success rate, can really reflect the properties of entire tumor cell groups, and can accurately represent the growth condition of in vivo tumors, and after the 3D culture system is used for medicine susceptibility detection, the obtained results are high in reliability, and clinical promotion is facilitated.

The invention relates to a portable anaerobion incubator. The portable anaerobion incubator comprises a box body, a single chip microcomputer, a control module, a display module, a power source circuit and a temperature and humidity sensor, a CO2 sensor, a heater and a moisturizer arranged in the box body; the control module, the display module, the power source circuit, the temperature and humidity sensor, the CO2 sensor, the heater and the moisturizer are all connected with the single chip microcomputer; the control module comprises a plurality of press buttons and a connection circuit enabling the multiple press buttons to be connected with the single chip microcomputer. The portable anaerobion incubator provided by the invention is scientific and reasonable in design, easy and convenient to operate, can conduct accurate and stable automatic control and adjustment on the temperature and humidity and the CO2 concentration in the box body, and guarantees that the conditions of the environment beneficial for bacteria to grow remain in the most proper range, the bacteria culture success rate is high, and the requirements for practical application can be well met.

The invention discloses a leaf cutting propagation method of succulent plants of xPachyveria pachytoides, and belongs to the technical field of a planting propagation method. The method provided by the invention is characterized in that a leaf of a xPachyveria pachytoides mother plant subjected to shade still standing is pulled and taken, and is then subjected to air drying for drying the broken edge; then, the back side of the obtained leaf is in contact with culture soil prepared in advance, and is then subjected to indoor scattered light culture; when roots being 5mm grow from the leaf, planting soil is used for covering the root parts; after the number of the leaves exceeds eight, and the plant reaches the height of 2cm, the foliage fertilization is performed; after the plant continuously grows by at least 1cm, the transplanting is performed, wherein the culture soil is prepared by uniformly mixing sand, coal slag and peat soil which are dried in the air for 2 to 3 days and have the same weight. The method provided by the invention has the characteristics that the operation is simple; the propagation period is short; the survival rate is high, and the like. The method is very suitable for large scale culture.

The invention relates to a culture medium of amniotic fluid cells, which belongs to the field of biochemistry. The culture medium comprises the following components: basic medium, transferrin, sodium selenite, insulin, triiodothyronine, glucagons, basic fibroblast growth factor, hydrocortisone, testosterone, estradiol, progesterone and added antioxidant, wherein the antioxidant is a mixture of vitamin E and L-ascorbic acid; and the culture medium can be used in amniotic fluid cell culture after 4 to 15 volume percent of fetal calf serum is added in the culture medium, and a great amount of amniotic fluid cells which are at a division stage and meet the requirements of clinical diagnosis and scientific research can be obtained just within 7 to 8 days. The culture medium remarkably improves the culture effects of amniotic fluid cells and obviously increases cloning efficiency and cloning growth rate, reduces culture time, increases the success rate of cell culture, and fully plays the synergetic effects of vitamin E and L-ascorbic acid of different antioxidants.