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Method for improving success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells

A technology of mesenchymal stem cells and success rate, which is applied in the field of using placental mesenchymal stem cells to improve the success rate of umbilical cord blood mesenchymal stem cells, and can solve the problems of increased difficulty in purification of cell culture end products, contamination of exogenous viruses and pathogenic factors, The composition of fetal bovine serum is complex and other problems, to achieve the effect of shortening the attachment time, accelerating the growth rate of cells, and expanding the value of use

Inactive Publication Date: 2015-06-24
中国医科大学 +1
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Problems solved by technology

However, due to the complex composition of fetal bovine serum, the use of serum-containing medium to culture cells has potential cytotoxicity, foreign virus and pathogenic factor contamination, which makes the standardization of cell culture and the purification of final products more difficult, and the residue of heterogeneous serum clinically allergic reactions

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  • Method for improving success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells
  • Method for improving success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells
  • Method for improving success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells

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Embodiment Construction

[0048] The present invention will be further described in detail below in conjunction with specific examples.

[0049] The invention provides a method for improving the success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells, comprising the following steps.

[0050] Step 1, collection and transportation of the placenta.

[0051] Screening of donors: Healthy fetal placenta tissue from full-term caesarean section was collected from the Department of Obstetrics and Gynecology of a hospital in Shenyang after informed consent of the parturient, stored at 4°C, and transported to the laboratory within 24 hours. Maternal blood should be collected within 7 days before delivery for AIDS testing, hepatitis B testing, hepatitis C testing, cytomegalovirus testing, syphilis testing, alanine aminotransferase testing, and mycoplasma testing.

[0052] Step 2. Wash the placental tissue with normal saline in a biological safety cabinet to rem...

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Abstract

The invention relates to a method for improving the success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells. The method comprises the following steps: firstly, digesting placentallobules in a short time by using collagenase I to obtain a paste mixture consisting of extracellular matrix protein and tissue blocks and carrying out primary culture; and then co-culturing third-generation placental mesenchymal stem cells as moisturizing cells and separated primary umbilical cord blood mesenchymal stem cells under a low-oxygen condition to obtain a lot of umbilical cord blood mesenchymal stem cells. The method for improving the success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells provided by the invention shortens the culture time of the placental mesenchymal stem cells, effectively solves the problem that the placental mesenchymal stem cells are low in purity and poor in multiplication capacity and the like, improves the success rate of umbilical cord blood mesenchymal stem cell culture and develops the utilization value of the umbilical cord blood mesenchymal stem cells. As fetal calf serums and pancreatin are not used, the safety of the cells on clinical application is enhanced.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for improving the success rate of umbilical cord blood mesenchymal stem cell culture by using placental mesenchymal stem cells. Background technique [0002] Stem cells are primitive cells with certain self-renewal ability and multi-directional differentiation potential in a suitable microenvironment, which can differentiate into fat, cartilage, bone, muscle, tendon, nerve, liver, cardiac muscle and other tissue cells. Mesenchymal stem cells (mesenchymal stem cells, MSCs) are non-terminal differentiated cells derived from mesoderm, which have the characteristics of mesenchymal cells, endothelial cells and epithelial cells, and are an important source of seed cells for cell therapy and tissue engineering . In addition to bone marrow, peripheral blood, fat, and umbilical cord, human placenta is also an important source of mesenchymal stem cells. In addition to the above-menti...

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 荣耀星时兆田于艳秋李秀文邓雯雯张停停吕亚楠
Owner 中国医科大学
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