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100 results about "Placental tissue" patented technology

Stents modified with material comprising amnion tissue and corresponding processes

A stent scaffold combined with amniotic tissue provides for a biocompatible stent that has improved biocompatibility and hemocompatibility. The amnion tissue can be variously modified or unmodified form of amnion tissue such as non-cryo amnion tissue, solubilized amnion tissue, amnion tissue fabric, chemically modified amnion tissue, amnion tissue treated with radiation, amnion tissue treated with heat, or a combination thereof. Materials such as polymer, placental tissue, pericardium tissue, small intestine submucosa can be used in combination with the amnion tissue. The amnion tissue can be attached to the inside, the outside, both inside and outside, or complete encapsulation of the stent scaffold. In some embodiments, at least part of the covering or lining comprises a plurality of layers of amnion tissue. The method of making the biocompatible stent and its delivery and deployment are also discussed.
Owner:PEYTANT SOLUTIONS INC

Method for freezing and thawing placental whole cells and separating and expanding stem cells

The invention relates to a method for freezing and thawing placental whole cells and separating and expanding stem cells. The method comprises the steps as follows: disinfecting and washing a placenta tissue; cutting off placental lobules from the tissue, and carrying out digestive treatment for 20 min; preparing a placenta tissue freezing solution for standby application; adding whole cells obtained by digestive treatment and the freezing solution into a freezing tube, refrigerating for 0.5 h at the temperature of 4 DEG C, freezing for 1 day at the temperature of subzero 80 DEG C, and freezing in liquid nitrogen for standby application; taking out the placental whole cells from the liquid nitrogen when needed, thawing in a thermostatic water bath, carrying out drop-method washing by a culture medium for mesenchymal stem cells, removing red cells by a red cell lysis solution, and expanding the mesenchymal stem cells by the thawed placental whole cells through cell culture and cell passage. According to the method, the frozen placenta tissue can be effectively protected and is convenient to thaw and use; and the method is in particular suitable for separating and expanding the mesenchymal stem cells after thawing the frozen placental tissue.
Owner:BOYALIFE

Placenta stem cell bank construction method and placenta tissue resuscitation method

The invention discloses a placenta stem cell bank construction method and a placenta tissue resuscitation method, and relates to placenta stem cell bank construction methods. The placenta stem cell bank construction method comprises the following steps: after non-bacterial cleaning treatment to placenta tissues of at least three parts, the steps of tissue peeling, further sterilizing, shearing to be thin, protecting using a refrigerant, programmed freezing, cryogenic temperature temporary storage, long term storage with nitrogen canisters, and the like are carried out, and the placenta tissue with biological activities can be preserved for a long term. The placenta tissue preserved through the method can be used for the construction of a stem cell bank, resuscitation, enzymic digestion, cultivation, expansion, quality inspection and the like are performed after selective tissue part resuscitation according to required cell types, so as to obtain placenta amniotic membrance epithelial cells, placenta amniotic membrance mesenchyme, placenta chorion mesenchymal stem cell, placenta decidua serotina mesenchymal stem cells and the like. The method provided by the invention has the advantages that operation steps are simplified, manual intervention errors are reduced, the efficiency is improved, more stem cell resources are preserved, and a basis is provided for further personalized stem cell customization.
Owner:天晴干细胞股份有限公司

Cell type used for producing induced pluripotent stem (iPS) cells and preparation method and application thereof

InactiveCN101984050AGreat therapeutic application prospectsGood pluripotencyFermentationVector-based foreign material introductionControl orientedDisease
The invention discloses four cell types which have wide heteroplastic transplantation application prospect and can be effectively induced into induced pluripotent stem (iPS) cells. The origins of three cell types are placental tissue, namely amnion mesenchymal cell, chorion mesenchymal cell and umbilical cord mesenchymal cell; and the origin of the other one is amniotic fluid cell. Besides, the invention also discloses an induction reprogramming method for producing cells capable of producing induced pluripotent stem (iPS) cells by efficient induction, including the following steps: cDNA containing pluripotent stem cell factor is respectively introduced into the four primary culture cells; the four primary cells in which cDNA is introduced are respectively cultured on appropriate culture mediums, primary iPS is obtained and then quality of iPS is optimized on appropriate culture mediums, and cloning of pluripotent stem cell can be primarily evaluated. In the invention, three mesenchymal cells of placenta origin and amniotic fluid cell all can be induced into iPS, wherein the chorion mesenchymal cell is compared with fibroblast, efficiency of induction reprogramming is improved by over 100 times, efficiency is about 2.3%, and the efficiency is slightly higher than that of horny cell; and a means which is more effective and more pertinent is provided for building disease model, screening drug and controlling oriented differentiation.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI

Separation method of human placenta mesenchymal stem cells

The invention discloses a separation method of human placenta mesenchymal stem cells. The separation method comprises the following steps that placenta specimen treatment is carried out, treated placenta tissue is obtained, tissue digestion is carried out on the placenta tissue, and cell suspension is obtained; preliminary subculture of the placenta mesenchymal stem cells is carried out; further separation and culture of the placenta mesenchymal stem cells are carried out. HyQTase and DNAse I are adopted for digestion together, the placenta mesenchymal stem cells are obtained through separation, after preliminary culture, microgravity treatment and electromagnetic field and sound wave treatment are carried out, BMP4 is used for treatment many times, upper layers are removed through a differential attachment method, a serum-free medium and antioxidant are replenished in a culture bottle for culture, and the final purity is high. The stem cell characteristics of the cells are verified through detection on hereditary stability, cell surface molecule expression conditions and cellular morphology in the continuous passage process, and materials are provided for seed cells with the placenta mesenchymal stem cells as clinical application.
Owner:大连金玛健康产业发展有限公司

Methods for early detection of pregnancy in cows

Disclosed are methods for determining the pregnancy status of a cow by detecting a pregnancy-associated glycoprotein (PAG) level that comprises proteins present in an acidic protein fraction of day 55 to day 60 bovine placental tissue. These methods may involve using novel polyclonal and monoclonal antibodies that have been generated using the acidic protein fraction as an immunogen. According to the method, the cow is pregnant when the PAG level is present at an elevated level in a sample obtained from the cow. The invention provides accurate methods of detecting pregnancy at early stages and has the benefit of allowing early post partum detection of pregnancy status with few false positive results. The antibodies for detecting the PAG level will also be useful in combination with detecting elevated progesterone levels, thus providing an even more effective pregnancy detection method. The benefit of this early pregnancy detection is that identifying cows that are not pregnant very shortly after breeding allows for timely rebreeding and minimizes the amount of time the cow is open. Also disclosed are methods for making a breeding decision for a cow. These methods allow a herd manager to make breeding decisions based on the PAG level and optionally, progesterone levels, detected in a biological sample taken from a cow suspected of being in the early stages of pregnancy.
Owner:MONSANTO TECH LLC

Amniotic membrane mesenchymal stem cell release molecule (ASRM) as well as preparation method and application of ASRM

The invention discloses a sheep stem extract and its preparation method and application, and discloses a preparation method of its preparation freeze-dried powder. The sheep stem extract is obtained by separating and culturing amniotic mesenchymal stem cells from human placenta amniotic tissue, and using human platelets The lysate is cultured and expanded, and then the culture supernatant of human amniotic mesenchymal stem cells is collected, concentrated and collected, which solves the problems of long doubling time and poor multi-differentiation potential of AMSCs, and can qualitatively and Quantitative analysis can improve the active ingredients in sheep stems, such as antioxidants and various growth factors, and prevent the active ingredients from degrading. The results of animal experiments and clinical trials show that the sheep dry extract prepared by this technology can be used to delay aging, prevent and treat osteoporosis and skin aging, and promote skin wound healing.
Owner:NANJING MEDICAL UNIV
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