967results about How to "Improve enzymatic hydrolysis efficiency" patented technology

The invention discloses a high-purity fishy smell and foreign odor-free fish collagen protein peptide and a preparation method thereof. The preparation method comprises the following steps: cleaning fish skins, and then cutting into blocks and mincing; performing stirring treatment with NaCl solution; centrifugally degreasing to remove paraprotein; adding water to regulate the pH value of initial slurry to be 8.0-8.5; performing combined gradient enzymolysis on alkali protease and neutral protease; deactivating enzyme after the enzymolysis is ended; performing adsorption bleaching by activated carbon; and then performing rough filtration, fine filtration, evaporation concentration and spray drying to prepare the high-purity fishy smell and foreign odor-free fish collagen protein peptide. The high-purity fishy smell and foreign odor-free fish collagen protein peptide disclosed by the invention has a simple process and is easy to industrial production; fishy smell, foreign odor and non-collagen proteins are fully removed in production; the low-temperature biological enzymolysis technology is adopted, and other substances are not added, thereby, the product quality is improved; an obtained fish collagen protein peptide powder has the characteristics the content of amino acid hydroxyproline is not less than 9 percent, and the average molecular weight is lower than 1000Dal; no fishy smell or foreign odor cannot generated, and obvious fishy smell or foreign odor also cannot be generated through heating treatment under the acid conditions. The high-purity fishy smell and foreign odor-free fish collagen protein peptide can be widely applied to processing of various foods and cosmetics as a green functional ingredient.

The invention discloses a sea cucumber polypeptide functional food and a preparation method thereof which not only remarkably improves the content of polypeptide, but also has no chemical residues; the contents of salt and arsenic are low; the food is healthier and safer to eat; simultaneously the food is simply operated, easily controlled, is effective and saves energies. The key technical scheme includes: selecting a compound protease of Protamex and needing not to adjust the pH value of materials; carrying out processes of desalting and arsenic removing on an enzymolysis liquid; more than 80 percent of the molecular weight of the product is between 100 to 6000Dalt; wherein, the small polypeptide of 100 to 2100Dalt is more than 70 percent; the product components and the content weight percentages are as follows: 50 to 60 percent of polypeptide, 5 to 10 percent of free amino acids, 2.5 to 7.5 percent of mucoitin as well as containing the inherent nutrition components of a plurality of minerals and vitamins of the sea cucumber. The product has the effects of resisting knub, reducing blood pressure, preventing cardio-cerebrovascular diseases, resisting fatigue, delaying senescence, improving the immunity. The food can be used as a healthy food to eat and can also be used as a food and a medicine additive.

The invention relates to a maize oligopeptide and a method for preparing same, belonging to the field of biological technology. The method takes the byproduct, namely corn protein powder (also called maize yellow powder containing about 60% of protein) in corn starch production as a raw material, removes the impurities such as starch, grease and the like by the treatment of alkaline water and obtains high-purity corn protein slag; furthermore, the corn protein slag is taken as enzymatic hydrolysis substrate and treated by the following process of pre-treatment, enzymatic hydrolysis, multi-step separation and purification, concentrating, drying and the like so as to prepare the maize oligopeptide powders with edible value and various health-care functions. The method for preparing maize oligopeptide has the advantages of easily obtaining raw material, simple process and higher industrial feasibility. The prepared maize oligopeptide sample has high crude protein content and small molecular weight, has various physiological activities of prompting immunity adjustment, anti-fatigue and the like, and can be used as new functional nutritional compounding and widely applied to the development and production of products such as foods, health-care foods and the like.

The invention discloses a technology for preparing broth powder by cooking and enzymatic hydrolysis, which comprises the steps of carrying out the working procedures of cooking, ultrafine smashing, enzymatic hydrolysis, Maillard reaction, micro-encapsulation, spray drying and the like on small pieces of bones and meat of plain chicken which removes the head, the paws and the internal organs and seasonings of vegetable oil, onion, ginger, garlic and the like, thereby preparing the broth powder. The cooking technology is applied for enhancing the flavor and the taste of the broth powder, thereby increasing the mellow, thick and fragrant base flavor of the broth powder; the superfine smashing is carried out on the materials, thereby increasing the enzymatic hydrolysis efficiency, increasing the content of small molecular peptides and calcium and leading the nutrition thereof to be more conductive to human body absorption; a composite enzyme preparation is added for carrying out the enzymatic hydrolysis, thereby effectively degrading meat proteins into the small molecular peptides and amino acids; and the microencapsulation is conductive to retaining the flavor of the broth powder for a long time, being easy to dissolve and extending the guarantee period. The broth powder prepared by the technology is characterized by meat flavor, mellow and thick base flavor and abundant amino acids, small molecular peptides and other nutrients, thereby being an essential cooking raw material for the hospitality industry and also an essential seasoning for home cooking.

The invention discloses a method for preparing an antioxidant active peptide by hydrolyzing fish scale collagen with co-immobilized double enzymes. The method comprises the following steps of: (1) co-immobilizing double enzymes by cross-linking method, (2) pre-treating fish scale, (3) hydrolyzing fish scale proteins with the co-immobilized double enzymes, (4) decolorizing and deodorizing, (5) separating fish scale polypeptides by ultrafiltration, (6) vacuum concentrating; and (7) freeze drying. The antioxidant active peptide product can be used as a natural antioxidant in antioxidation and preservation of foods and has no toxic or side effect and high safety. The method has high enzymolysis efficiency, the enzymes can be used repeatedly, and the enzymatic hydrolysates are purified easily.The method provides a new approach to effective utilization of fresh water fish waste, and the product can be used in pharmaceuticals, foods and cosmetics.

The invention discloses a device and a method for biological processing with pulsed multi-frequency ultrasonic waves in a sweep-frequency mode and use thereof, which relate to the field of agricultural product deep processing. The device consists of an ultrasonic wave processing system, an ultrasonic wave generating system and a computer control system, which are sequentially connected by cables, wherein the ultrasonic wave processing system consists of an upper ultrasonic wave transducer, a lower ultrasonic wave transducer, an ultrasonic wave processing tank body, a circulating pump and an upper oscillatory plate height adjusting screw rod; an ultrasonic wave generator set consists of a group of pulsed ultrasonic wave generators at different frequencies; and the control system consists of an ultrasonic wave controller, a temperature controller and a computer. The equipment can be used in ultrasonic wave-assisted extraction of active ingredients of natural products and the extraction efficiency is improved 10 to 90 percent. The equipment can be used in ultrasonic wave-assisted enzymolysis of biological macromolecules, wherein the ultrasonic wave-assisted enzymolysis includes preprocessing raw materials, preprocessing hydrolases and performing enzymolysis in an ultrasonic wave field; and the enzymolysis efficiency is improved by 25 to 65 percent.

The invention relates to a method for breaking walls of cells of bran by wet grinding, and belongs to the technical field of industrial biochemistry. The invention adopts the technical key point that: bran, water, and enzyme for degrading walls of cells of the bran are mixed, and subjected to wet ball-milling grinding to form superfine wall-breaking powder. By combining an ultramicro-pulverization method, the water and the enzyme for degrading the walls of cells of the bran, the cells of the bran are subjected to wet ball milling for wall-breaking treatment; compared with single ultramicro pulverization, the method can greatly reduce the energy consumption, is simple and has low requirement on operating equipment; and compared with single enzymatic degradation, the method combines wet ball milling, combines chemical action with mechanical force action, and has the advantages of greatly saving energy required by high temperature treatment, avoiding the defect of enzyme inactivation under high temperature treatment, eliminating crystal structures of cellulose and improving enzymolysis efficiency.

The invention relates to a method for pretreating lignocellulose by adopting an alkaline eutectic solvent. With the method, the enzymolysis efficiency of lignocellulose can be effectively enhanced, particularly, the recycling for the hemicellulose sugar monomer is improved, and the yield of total sugar is improved. The method has the advantages that the cost is low, the operability is strong, theenergy consumption is small, the environment protection is realized, etc.

The invention discloses a cosmetic containing oceanic shellfish active peptide and a preparation method and application thereof. The cosmetic contains 0.1 to 10 percent of oceanic shellfish active peptide, or 0.1 to 10 percent of oceanic shellfish active peptide and 1 to 10 percent of hyaluronic acid, or 0.1 to 10 percent of oceanic shellfish active peptide, 1 to 10 percent of hyaluronic acid and1 to 25 percent of vitamin C (VC), and the balance is cosmetic matrix components. The peptide is formed by boiling, ultrasonic, enzymolysis and ultra-filtration of oceanic shellfish meat; and besidesthe active peptide, the oceanic shellfish meat also contains free amino acid, glycosaminoglycan, polyunsaturated fatty acid, vitamins, trace elements and the like. The problems of application and thelike of the oceanic shellfish active peptide in the cosmetic are solved; and the prepared cream, emulsion, aqueous agent, gel, aerosol or mask has more complete and remarkable skin-care or hair-care effect. After the hyaluronic acid and the VC are added, the effects of preserving moisture, increasing the elasticity and the smoothness of the skin, removing flaws and improving the skin color are remarkably enhanced.

The invention discloses an efficient enzyme hydrolysis method of lignocellulose biomass, comprising the following steps: pretreating the lignocellulose biomass through high-temperature aqueous water to obtain a lignocellulose raw material to be used as a substrate; dispersing the substrate into an acetic acid-sodium acetate buffer solution in a certain concentration; adding a nonionic surfactant and cellulose; stirring the materials; hydrolyzing for some time; adding a certain amount of materials twice; and simultaneously adding the nonionic surfactant and the cellulose; and continuously hydrolyzing to obtain a saccharide compound and lignocellulose residues. In the method provided by the invention, high-temperature aqueous water pretreatment, batch charging, nonionic surfactant adding and stirring and an enzyme hydrolysis process are coupled, thus the hydrolysis efficiency is high, the concentration of the enzymolysized lignocellulose raw material can be greatly improved; the concentration reaches 30%; the sugar generation concentration and the hydrolysis efficiency are high under the condition of high-concentration lignocellulose raw materials; the enzymolysized raw material concentration is improved; and simultaneously the high-concentration sugar is produced, thus improving the hydrolysis efficiency, and realizing the efficient enzyme hydrolysis of the lignocellulose biomass.

The invention discloses a method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment, which comprises the following steps: firstly, performing pretreatment on the rice straws at certain temperature for a certain period of time by taking choline and amino acid ionic liquid as a solvent; then filtering and separating to obtain residues; drying the residues to obtain the pretreated rice straws. The residues of the rice straws are used as substrates, and enzymolysis is performed on the substrates by utilizing cellulase and xylanase to finally obtain sugar liquor taking glucose and xylose as main components. The method for increasing the enzymolysis efficiency of the rice straws through the choline and amino acid ionic liquid pretreatment not only is capable of effectively increasing the enzymolysis efficiency of the rice straws and increasing the yield of reducing sugar (the glucose and the xylose) which can be fermented, but also is capable of overcoming the defects that a traditional ionic liquid pretreatment process environment is not friendly and the like.

The invention discloses a method for preparing ultra-low molecular weight hyaluronic acid oligosaccharides and a salt thereof through combination of solid-liquid biphasic enzymolysis and ultrafiltration. The method comprises the following steps: degrading hyaluronic acid and a salt thereof by utilizing hyaluronidase produced by bacilli; preparing high-concentration hyaluronic acid enzymatic hydrolysate by adopting the method of combining a solid-liquid biphasic enzymolysis system and an ultrafiltration system, and then performing inactivating, activated carbon adsorption and impurity removal,filtering and spray-drying to obtain the ultra-low molecular weight hyaluronic acid oligosaccharides and the salt thereof with the molecular weight of less than or equal to 3 kDa. The product preparedby the method is high in stability, ultra-low in molecular weight, good in inflammation resistance and percutaneous absorptivity, high in purity, strong in oxidation resistance, does not have cytotoxicity, has the advantages of repairing skin cell damage and the like, and can be widely applied to the fields of cosmetics, food and medicines. According to the method, the process flow is easy to operate; conditions are mild; various alcohols are not used; the energy consumption is relatively low; the method does not have damage to a product structure, does not have environmental pollution, and is suitable for large-scale industrial production.

The invention relates to an in-situ rapid preprocessing method for protein samples. The whole preprocessing process of the protein samples is carried out in a centrifugal ultrafiltration device in situ, the denaturation process, the reduction process, the solvent replacement process, the alkylate process, the enzymolysis process and the impurity removal process of the protein samples are integrated, and enzymolysis of the protein samples is accelerated under assistance of microwaves. The method can remarkably improve the preprocessing flux of the protein samples; meanwhile, the whole preprocessing process of the samples is carried out in situ, and the recovery rate is high. In addition, the centrifugal ultrafiltration device can conveniently and rapidly achieve the purification of the samples and replacement of solvents, and enzymatic hydrolysate does not need an additional purification process and can be directly combined with subsequent efficient liquid chromatogram separation and mass spectrum identification.

The invention relates to an environmentally-friendly method for preparing shellfish (such as abalone, scallop and the like) viscera fat by an aqueous enzymatic method. The invention mainly takes fresh shellfish viscera as raw material, and comprises the following steps: firstly, nutritive cells in the shellfish viscera are broken by the frozen-thawed technology; endoenzymes, such as protease released after breaking cells and the like are utilized for autolysis; exogenous enzymes are added into autolysis solution for further degradation; after an isoelectric point method is combined with salt fractionation for demulsification, high speed centrifugation is carried out to take an upper oil layer to prepare the shellfish viscera fat; and finally, the supercritical CO2 extraction method is adopted to refine the shellfish viscera fat. The invention applies the aqueous enzymatic method into the preparation technology of the shellfish viscera fat for the first time. The method has the advantages of environmental protection, low production cost and moderate process condition, can furthest keep the polyunsaturated fatty acid in the shellfish viscera fat and causes the product to have trophism and functionality.

The invention discloses a combined pretreatment method for efficiently separating bagasse biomass components, which mainly comprises the following steps: preimpregnating in dilute sulfuric acid; carrying out steam explosion treatment; washing with hot water; extracting; and carrying out enzymolysis. In the combined pretreatment method disclosed by the invention, on the basis of different physicochemical properties and structural features of the three components (hemicellulose, cellulose and lignin) in the bagasse, the component hemicellulose is sufficiently degraded and separated by dilute acid preimpregnation-steam explosion pretreatment, thereby reserving most of the cellulose and lignin; and the lignin is extracted by using a lower alcohol-alkaline water system, leaving high-cellulose-content residues. On the premise of low concentration of the added enzyme, the invention obviously increases the enzymolysis rate, and raises the conversion rate and comprehensive utilization rate of the bagasse biomass resource.

The invention relates to a rice bran soluble dietary fiber, a production technology and application thereof, and belongs to the field of comprehensive utilization and deep processing of grains. The production technology comprises the following steps: rice bran steam explosion pretreatment, enzymolysis, extraction, concentration, alcohol precipitation and drying. The obtained dietary fiber has higher water-holding capability, oil-holding capacity and expansive force, improves the physiological activity of reducing blood sugar, reducing cholesterol and oxidation resistance and the like, and can be widely applied to the fields of food, health care products and the like.

The invention provides a method for preparing antioxidant oligopeptide by performing ultrasonic enzymolysis on swim bladders. The method comprises the following steps of: crushing the swim bladders into swim bladder powder; adding a phosphate buffer solution with the pH value of 6.0 and trypsin into the swim bladder powder and stirring uniformly to obtain a swim bladder solution; performing enzymolysis on the swim bladder solution in water bath; performing ultrasonic treatment on the swim bladder solution for enzymolysis; after ultrasonic treatment, continuously performing enzymolysis in constant-temperature water bath; after the enzymolysis is finished, deactivating enzyme, cooling and filtering to obtain supernate; performing ultrafiltration on the supernate by using a 10,000-Da ultrafiltration membrane; and freeze-drying the solution obtained after ultrafiltration to obtain the swim bladder antioxidant oligopeptide. The antioxidant oligopeptide prepared by the method has a good effect of eliminating free radicals of a human body, can prevent ageing, inflammation and heart cerebrovascular diseases caused by arteriosclerosis, and can serve as a nutritional additive to be applied to food.

The invention discloses a production method for convenient rice with a favorable retrogradation resisting performance, which comprises the steps of raw material rice washing, primary soaking, primary cooking, secondary soaking, secondary cooking, dispersing, drying and packaging, wherein a primary soaking solution is a water solution containing 0.2-0.8 percent by weight of beta-cyclodextrine and 0.1-0.4 percent by weight of monoglyceride; and a secondary soaking solution is a water solution containing 0.2-0.8 percent by weight of beta-cyclodextrine, 0.1-0.4 percent by weight of monoglyceride,1000-8000U / 100g of alkali protease of raw material rice and 300-3000U / 100g of beta- cyclodextrine of the raw material rice. According to the production method disclosed by the invention, the process is simple, the favorable retrogradation resisting performance of the dehydrated convenient rice can be improved, the mouthfeeling of the rice is improved and the rehydration time of the convenient rice is shortened; and the production method is more convenient to use.

The invention discloses a method for extracting high-activity lignin from biomass and lignin extracted by the same. The method comprises the following steps: (a) obtaining high-purity and high-activity lignin from a biomass raw material ground to 20-60 meshes by using an alcohol organic solvent through an acid liquid precipitation way; and (b) hydrolyzing the residue obtained in the step (a) with cellulase at 50 DEG C for 72h to obtain high-yield fermentable glucose for bioethanol preparation. In the invention, pretreatment using isopropyl alcohol is creatively proposed under specific conditions to break a lignin-carbohydrate bond and a beta-O-4-aryl ether bond so as to efficiently separate and extract low-molecular weight high-activity lignin, and theoretical basis and technical support are provided for further high-value utilization. Moreover, the pretreatment method remarkably improves the enzymolysis efficiency of the fiber raw material and provides an effective way for next bioethanol production.

The invention relates to a method for pre-treating lignocellulose raw material and for preparing reducing sugar by conversion. With liquid-solid ratio of 5-50, lignocellulose is treated for 1-10h in a mixed solution of 0-5% of sodium hydroxide by mass and 0-5% of hydrogen peroxide by mass; solid residue obtained by treating is washed by using water, and is dried by baking; the obtained material is treated under a liquid-solid ratio of 5-50, an acid hydrolysis time of 10-120min, an acid hydrolysis temperature of 30-150min, and a sulfuric acid mass concentration of 0-10%, such that a solid residue is obtained; a pH value is regulated to 7; and the obtained material is dried by baking. With the treatment process, lignin can be effectively removed, and hemicellulose is hydrolyzed under a dilute acid condition, such that pentose is obtained. With further reactions, products with high added value can be obtained. With a sample treated with the process, ineffective adsorption upon cellulase is reduced, and a conversion rate of cellulose in preparing ethanol can reach 67%. Therefore, a production cost is relatively reduced.

The invention discloses a bagasse pretreatment method. The bagasse pretreatment method comprises the following steps of: drying, crushing and screening the bagasse, then pretreating the bagasse by using sodium hydroxide and hydrogen peroxide to obtain the pretreated bagasse. According to the pretreated bagasse material, the maximum removal rates of the hemicellulose and lignin respectively reach 78.18 percent and 94.56 percent, the maximum enzyme hydrolysis rate of the cellulose is 64.37 percent, and the enzymolysis efficiency is improved by 60.67 percent compared with that of untreated raw bagasse. Therefore, the pretreatment method breaks through wrapping of the hemicellulose and lignin to the cellulose, the hydrolysis rate of the cellulose is increased, the enzymolysis resistance property of the bagasse is improved, the obtained bagasse material can be used for converting high added value products, such as ethanol through microbial fermentation after being subjected to enzymolysis, and thus biomass resources, such as bagasse are effectively utilized.

The invention discloses characteristic peptide of peanut allergen protein and application thereof. The amino acid sequence of the characteristic peptide is DLAFPGSGEQVEK or NLPQQCGLR. Allergens which are the highest in content in peanuts, namely Arah1 and Arah2, are selected as candidate markers, the characteristic peptide of Arah1 and the characteristic peptide of Arh2 are screened out, corresponding internal standard substances are designed and synthesized, the analysis technology in which the high performance liquid chromatography and mass spectrum are used in a combined mode is adopted, and the content of the peanut allergen protein in food is calculated and obtained. A method of the application has good linear property and high sensitivity, recovery rate and precision degree.

The invention provides a preparation method of a lactose-free fermented dairy product. The method comprises the following steps: blending, homogenizing, sterilizing, carrying out enzyme-hydrolyzing, fermenting, filling and refrigerating all the raw materials in sequence to obtain the lactose-free fermented dairy product, wherein the enzymatic hydrolysis and fermentation steps comprise the following processes: adding lactase at the temperature of 35-40 DEG C, hydrolyzing till the degree of hydrolysis is above 80 percent, and then adding stains; or adding lactase at the temperature of 41-45 DEG C, hydrolyzing till the degree of hydrolysis is above 95 percent, and then adding stains; fermenting till the pH value of feed liquid is 5.5, starting to inactivate the lactose and ending the fermentation when the pH value of the feed liquid is 4.3-4.5; or adding lactose and medium temperature strains at the temperature of 30-35 DEG C, fermenting till the pH value of feed liquid is 5.5, then rising the temperature to 40-45 DEG C and adding thermophilic stains, and ending the fermentation when the pH value of the feed liquid is 4.3-4.5. The preparation method has the benefit that through addition of the lactose and improvement of a fermented milk preparation process, the lactose-free fermented dairy product can be effectively prepared and obtained.

The invention provides a method for performing lignocelluloses raw material high-temperature diluted acid pretreatment by using extremely small amount of water, which comprises the following steps: (1) pre-treating a lignocelluloses raw material to remove impurities; (2) mixing the raw material with 0.2 to 10 weight percent acidic solution in an extremely high solid to liquid ratio, uniformly stirring, and standing at normal temperature to infiltrate the acidic solution into the lignocelluloses raw material completely, wherein the extremely high solid to liquid ratio is 1:2-3:1; and (3) filling the obtained raw material into a pretreatment reactor at a 100 percent material filling rate, heating the raw material to a required temperature and pressure directly by high-temperature steam, keeping the temperature and pressure for a certain period of time, relieving pressure, taking the material out and cooling. When the method is used, water consumption and energy consumption are reduced greatly on the premise of ensuring the pretreatment effect, zero discharge of sewage is realized, a pretreated lignocelluloses raw material with high solid content is obtained, and the perfect connection with a subsequent high-solid-content biological processing process is realized.

The invention discloses beef marrow bone zymolyte and an application thereof in preparation of beef flavor. The beef marrow bone zymolyte is prepared by protease enzymolysis by taking beef bone mud as a raw material; the protease comprises neutral protease, flavourzyme and papain in the weight ratio of (3 to 5): (2 to 5): (2 to 5); the enzymolysis temperature is 45 to 55 DEG C; the enzymolysis time is 2 to 3 hours; and the beef bone mud is prepared by grinding the beef marrow bone. The beef flavor prepared from the beef marrow bone zymolyte disclosed by the invention through a Maillard reaction has unique beef marrow characteristic aroma; the fragrance is lasting and the flavor is unique; and flowing beef breath smell can be generated.

The present invention discloses enzyme extracting process of edible fungus leaching juice. The enzyme extracting process includes the following steps: mixing edible fungus in 24-34 wt% and water in 65-75 wt%, pulping, adding citric acid in 0.05-0.2 wt% and vitamin C in 0.02-0.1 wt%; heating at 60-80 deg.c to leach for 30-90 min before cooling to 45-55 deg.c, regulating pH value to 5.0-6.5 with 10 % concentration hydrochloric acid solution, adding compound cellulose in 0.2-1.0 wt% and enzymolysis at 45-55 deg.c for 90-150 min; adding compound protease in 0.2-1.0 water and enzymolysis at 45-55 deg.c for 60-120 min; fast heating to 90 deg.c, maintaining for 10 min to deactivate enzyme; and final pressure filtering and vacuum concentrating to obtain the edible fungus leaching juice.

The invention discloses a method for producing ethanol by high-efficiency simultaneous saccharification and cofermentation, which comprises the following steps: pretreating; concentrating and detoxifying; preparing yeast seed solution; performing simultaneous saccharification and cofermentation; and distilling / rectifying. When the method provided by the invention is used, the inhibition action of a monosaccharide component formed by hydrolysis of cellulose on cellulose is relieved, the enzymolysis saccharification efficiency and fermentation yield are improved, the recovery rate of hemicelluloses in bagasse is about 60 percent, the cellulose recovery rate is about 86 percent, and the total ethanol yield of dry bagasse can reach about 20 percent.

The invention discloses preparation and application of an immobilized protease reagent. An immobilized enzyme disclosed by the invention is composed of a hydrophobic carrier material and a protease immobilized on the hydrophobic carrier material. Two different-property, namely one hydrophilic and one hydrophobic, polymer chain modified magnetic nanoparticle immobilized trypsins prepared independently by using a surface initiate atom transfer radical polymerization (SI-ATRP) realize quick, efficient and complete enzymolysis of a protein, and the two, one hydrophilic and one hydrophobic, immobilized proteases are combined to use so that complementary enzymolysis is realized, and therefore, enzymatic bias caused by the selectivity of the carrier can be effectively reduced, the comprehensiveness of protein enzymolysis can be improved, and then the identification number of proteins and peptide fragments can be remarkably increased.

The invention discloses a process method for modified collaborative preparation of egg white powder through enzymolysis and phosphorylation. The process method sequentially comprises the steps of egg cleaning, egg white separation, gauze filtration, distilled water dilution, constant temperature enzymolysis, enzyme deactivation treatment, enzymatic hydrolysate centrifugal filtration, phosphorylation, TCA (Trichloroacetic Acid) precipitation and the like. According to the method, the egg white is used as a raw material; the egg white powder which is easy to absorb, has small fishy smell and has good solubility, foaminess and emulsibility is prepared through the combined mode of the enzymolysis and the phosphorylation. The processing characteristic of egg white protein and the utilization rate of the protein are improved.

The invention discloses a method for producing a seaweed protein feed through the synergistic effect of enzymolysis and fermentation. The method comprises the steps of fully mixing seaweed residue (the water content is 38%) and a feed enzyme preparation according to a certain proportion and performing the enzymolysis for 4-7h with the pH of 6.2-7.0 at the temperature being 35-45 DEG C to obtain a seaweed residue-feed enzyme preparation mixture; inoculating compound microorganisms and performing solid fermentation. The method has a simple process and is easy to operate; an external source is added to degrade a cellulose preparation to produce nutrition beneficial to microbial growth through the enzymolysis and make up the deficiency of enzyme produced by using the compound microorganisms, so as to stabilize the solid fermentation process and the product quality, greatly reduce the content of cellulose and increase the protein content; the seaweed protein feed is rich in multiple minerals, bioactive constituents and unknown somatomedin, and can be used as a protein feed for livestock and poultry and an aquatic feed; the method provides a technical support for growing tensions on developing novel protein resources and relieving a feed protein source.