153 results about "Exogenous enzymes" patented technology

Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. Thelower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and / or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

The invention discloses a comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds, belonging to the process fields of grain and oil processing and byproduct extraction. The method comprises the following steps of: with shelled tea seeds as a raw material, firstly, cooking to produce oil; then, adding an exogenous enzyme to obtain a boiled oil layer of a first level an emulsified oil layer, a water layer and a slag layer through zymolysis, blanching and still standing; demulsifying the emulsified oil layer to take boiled oil of a second level; mixing the oil layers of the two levels; centrifugalizing to obtain the tea seed oil; depositing the water layer in alcohol to separate the tea saponin and the tea seed polysaccharide; leaching the slag layer with water or monohydric alcohol to further separate the tea saponin and the tea seed polysaccharide; and purifying a clear solution which is leached with the water or the alcohol with a macroporous absorption resin chromatography column, eluting a resin adsorbate with a base and then an ethanol and collecting an ethanol eluant to obtain high-purity tea saponin. The tea seed oil, the tea saponin and the tea seed polysaccharide are synchronously prepared, thus the invention realizes that a tea seed or camellia seed resource is comprehensively extracted and utilized and has the advantages of simple process, less needed equipment and low cost.

Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and / or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.

Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and / or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

The invention discloses a method for preparing bioactive protein powder through endogenous enzymolysis of marine low-value coarse fish, which is characterized by comprising steps of raw material preparation, primary crushing, enzymolysis, small peptide liquid preparation, mixing and drying, post treatment and the like. During enzymolysis, enzymolysis process parameters are selected according to the endogenous enzyme activity measured from the raw materials; and during mixing and drying, peeled bean pulp powder is used as a carrier to prepare a bioactive protein material. The method of the invention performs process improvement based on the enzymolysis of the primary exogenous proteinase preparation, solves the difficult problems of high production cost, unstable exogenous enzyme activity and non-thorough enzymolysis in the endogenous enzymolysis process, and is more suitable for industrialized production; and the prepared bioactive protein powder has the sense, flavor and cultivation effects superior to those of the fish powder, and is a good fish powder substitute.

The invention relates to an environmentally-friendly method for preparing shellfish (such as abalone, scallop and the like) viscera fat by an aqueous enzymatic method. The invention mainly takes fresh shellfish viscera as raw material, and comprises the following steps: firstly, nutritive cells in the shellfish viscera are broken by the frozen-thawed technology; endoenzymes, such as protease released after breaking cells and the like are utilized for autolysis; exogenous enzymes are added into autolysis solution for further degradation; after an isoelectric point method is combined with salt fractionation for demulsification, high speed centrifugation is carried out to take an upper oil layer to prepare the shellfish viscera fat; and finally, the supercritical CO2 extraction method is adopted to refine the shellfish viscera fat. The invention applies the aqueous enzymatic method into the preparation technology of the shellfish viscera fat for the first time. The method has the advantages of environmental protection, low production cost and moderate process condition, can furthest keep the polyunsaturated fatty acid in the shellfish viscera fat and causes the product to have trophism and functionality.

The invention discloses an antibiotic free, hormone free and harmful additive free biological mixed feed capable of improving quality of meat and suitable for feeding of various meat animals, and a production method thereof. The biological mixed feed comprises the following materials, by weight: 8-10% of biological fermentation cotton seed meal, 3-5% of polypeptide biological blood powder, 80-84% of mixed forage powder, 3-5% of feed additive and 1-5% of plant oil. According to exogenous enzyme and microbial fermentation, macro-molecule protein raw materials are decomposed into polypeptides and small peptides, so as to degrade anti-nutritional factors like non-starch polysaccharides in the forage and increase utilization rate of the forage; meanwhile, abundant B-group vitamin, free amino acid and organic acidity are generated during a fermentation process to promote deposition of flavor amino acid, inosinic acid and unsaturated fatty acid in muscle, so as to enhance quality of meat. Freshwater fish, fryers and growing and fattening pigs fed by the biological mixed feed for animals of the invention have characteristics of fast growth speed, and elastic, chewy, delicious and strongly fragrant fish meat, chicken and pork. The method of the invention is simple, requires no special equipment and is suitable for industrialized production.

The invention discloses a mixed composite microbial feed for freshwater fish and a production method thereof, wherein the mixed feed contains no antibiotics, no hormones and no harmful additives. The mixed composite microbial feed for freshwater fish comprises the following components, by weight, 8-10% of biologically fermented cottonseed rapeseed meal, 3-5% of polypeptide biological blood powder, 80-84% of mixed feed powder, 3-5% of a feed additive and 1-5% of plant oil. Macromolecular protein raw materials are decomposed into polypeptides and small peptides through an exogenous enzyme and the microbe fermentation, so antinutritional factors of non-starch polysaccharides and the like in the feed are degraded, and the feed utilization rate is improved; the feed contains no antibiotics, no hormones and no harmful feed additives; and the freshwater fish fed with the feed of the invention has the characteristics of fast growth speed and strong disease resistance. A mixed high protein biological feed for adulthood grass carp, which contains 35% or 32% of crude proteins, can be produced through above technology, the generation of hepatobiliary syndrome and three diseases of the grass carp is effectively reduced, and problems that the adulthood grass carp cannot be fed with the high protein feed and the feed of mix-cultured fish is difficult to select are solved; and the freshwater fish fed with the feed of the invention has the advantages of tough fish flesh and delicious taste. The method of the invention has the advantages of simplicity, no need of special equipment, and suitableness of the industrialized production.

Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce O-glycans or other structures along human glycosylation pathways. This is achieved using a combination of engineering and / or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

The invention discloses a preparation method of antarctic krill phospholipid. The preparation method comprises the steps: 1, draining and drying the antarctic krill and homogenizing; 2, irradiating homogenate liquid by using ultraviolet rays for 10-30 mins and autolyzing for 2-10 hours; 3, adding water into the homogenate liquid, ultrasonically processing, adjusting the pH value to be 7.5-8.0, adding alkali protease, and enzymatically hydrolyzing to obtain enzymolysis liquid; and drying the enzymolysis liquid to obtain dry powder; and 4, extracting the antarctic krill phospholipid by utilizing an ethanol extraction method. The preparation method is particularly suitable for use after fresh and alive antarctic krill are fished by fishing ships, and is simple in operation and high in efficiency. Particularly, the usage amount of exogenous enzymes is reduced by utilizing the strong autolysis capability of the fresh and alive antarctic krill, so that the production cost is lowered. Moderate conditions are provided for the extraction method, so that original physiological activators in the antarctic krill phospholipid can be stored to the maximum extent, and products are endowed with both nutritional and functional properties.

The invention discloses a sea cucumber egg nutriment and a preparation method thereof. The sea cucumber egg nutriment is white or light yellow hydroscopic powder free of odor and taste and highly soluble to water and ethanol. The preparation method comprises sea cucumber egg and intestine breaking and enzymolysis, metal ion removal through ion exchange, and freeze drying, wherein the enzymolysis employs conjugated enzymes of exogenous enzymes and endogenous enzymes or single exogenous enzymes; single exogenous enzyme enzymolysis refers to the enzymolysis using single pepsinase or using the mixture of trypsinase, dispase and alkaline proteinase. The invention not only makes wastes in sea cucumber processing with traditional methods into an invigorant rich in nutrition but also reduces pollution of the wastes to the environment. The processing method is simple and reasonable and can be industrialized easily.

The invention discloses a preparation method for instant black tea powder with high theaflavin content. The preparation method comprises the following steps: 1), taking dry green tea and adding hot water for extraction; filtering tea leaves to obtain tea soup; and cooling, centrifuging for separation and concentrating in sequence to obtain concentrated green tea juice; 2), taking fresh tea leaves and placing into a withering trough for withering to obtain withered leaves; 3), mixing the concentrated green tea juice with the withered leaves; rolling, grinding the rolled withered leaves and fermenting to obtain fermented leaves; and 4), adding the hot water into the fermented leaves for extraction, cooling, centrifuging for separation and concentrating to obtain concentrated black tea liquid, and drying to obtain the instant black tea powder. The instant black tea powder prepared by the method has the characteristics of high theaflavin content, unique flavor and fresh tea characteristics, does not need to be added with exogenous enzyme at the same time, and has the characteristics of low production cost, simple process and convenience in control.

The invention discloses an industrialized production method for instant brown rice. The method comprises the following steps of: firstly, carrying out the operation steps of drying, cleaning, grading, stone removing, rice ridging and husked rice separating on unhusked rice as the raw material so as to obtain the original brown rice; and secondly, carrying out soaking, enzymolyzing, curing, dryingand other processing means on the original brown rice to obtain the instant brown rice. In the invention, the common brown rice is used as the raw material to prepare the products by means of an endogenous enzyme germination technology, an exogenous enzyme hydrolysis technology, a brown rice pregelatinization technology, a rapid drying technology and the like; and the prepared products have the advantages of good palatability, short cooking time, long shelf life and the like. By adoption of the industrialized production method disclosed by the invention, the Alpha degree of the prepared brownrice can reach 100%; the cooking time of the brown rice is shorter than 15 minutes and the shelf life of the brown rice is more than 6 months; and the taste of the brown rice is favorably improved.

The invention discloses a flavor enhancement semi-fermented fleece-flower root leaf tea which is prepared by the following steps: (1) picking fresh leaves; (2) withering; (3) rocking of green leaves; (4) treating by exogenous enzyme for comprehensive flavor enhancement; (5) debitterizing and killing out; (6) mass rubbing; (7) baking by gross fire; (8) packed rolling; (9) baking by full fire; (10) drying. According to the preparation method of the flavor enhancement semi-fermented fleece-flower root leaf tea, galactosidase and water soluble chitosan are used for carrying out exogenous enzyme treatment on the fleece-flower root leaves for comprehensive flavor enhancement, so that the content of aromatic substances can be increased, and the free aroma can be locked on the leaves by the subsequent drying process so as to be avoided running away; the obtained flavor enhancement semi-fermented fleece-flower root leaf tea has the advantages of being green in color, elegant in flavor and durable in soaking, has very high nutritive value, medicinal value and health care effect, and is clear and elegant in mouthfeel; therefore, the flavor enhancement semi-fermented fleece-flower root leaf tea is a new semi-fermented tea, has very good application prospect, and has the advantages of being simple in preparation method, affordable and stable in product quality.

The invention discloses a dorking microorganism viable bacteria fermentation concentrated feed and a production method thereof. The dorking microorganism viable bacteria fermentation concentrated feed is prepared by mixing the following materials: 30-40% of biological decomposition bean pulp, 8-10% of polypeptide biological blood powder, 40-46% of mixed feed meal, 8-12% of feed additive and 1-5% of animal oil. In the feed, feed use ratio is improved by exogenous enzyme and microorganism fermentation; by the beneficial microorganism viable bacteria, flora in a dorking intestinal tract can be regulated to be balanced to improve the anti-stress capability of the dorking; and organic acid and bacteriocin generated by microorganism metabolism can inhibit and kill pathogenic bacteria in feed and the dorking body. The dorking microorganism viable bacteria fermentation concentrated feed does not need to add any antibiotic substance, can effectively inhibit undesirable bacteria growth, regulate the balance of egg and meat intestinal flora, and improve immunity; and chicken has abundant nutrition and does not contain any substance harmful to the human body.

A multienzyme high-efficiency concentration forage of the invention plays a part in straws or corncobs through utilizing a complicate enzyme system and microbes to improve the palatability of the forage; the pig house environment is improved through reducing the stink by supplementing insufficient exogenous enzymes in the alimentary canal of an animal via the complicate enzyme system; Chinese herbal medicines are added to obviously reduce the medicine and treatment cost; and effects of fast animal weight gain and culture cost reduction are reached. The high-efficiency multienzyme concentration forage comprises the following raw materials, by weight, 65-70% of soybean meal, 4-6% of powdery stone dust (containing 38.5% of calcium), 2-3% of calcium hydrogen phosphate, 0.12-0.15% of a composite vitamin, 0.8-1.0% of a mineral matter additive, 15-20% of DDGS, 0.1-0.2% of betaine, 6-10% of fermented straws or corncobs, 0.1-0.15% of a glucose oxidase, 0.05-0.1% of a protease, 0.05-0.1% of a phytase, 0.02-0.05% of a cellulase, 0.01-0.02% of a pectase and 0.05-0.1% of an amylase.

A method for improving the performance of enzymes used in animal feeds by the use of surfactants. Lecithin and / or lysolecithin is added to an animal feed including an exogenous enzyme to boost the performance of the enzyme so that a desired level of performance can be maintained while reducing the amount of exogenous enzyme that must be included in the animal feed. Preferably, the surfactant includes lyso-forms of lecithin.

The present invention relates to processes for the production of microbial glycolipids, mannosylerythritol lipids (MEL), from lignocellulosic carbon source. These processes are characterized in that the use of lignocellulosic materials for the production of a microbial glycolipids, MEL, comprising a fermentation preferably using fungi of the genus Pseudozyma or other microorganisms such as genetically modified fungi or bacteria. The processes for production of microbial glycolipids, MEL comprise three steps: pretreatment of lignocellulosic material; enzymatic hydrolysis; and fermentation. The enzymatic hydrolysis and fermentation may take place sequentially or simultaneously with addition of exogenous enzymes or simultaneously with enzymes produced by the microorganism itself. The produced microbial glycolipids have applications as: biosurfactants; antimicrobials; anticancer agents; wound healing factors; stabilizer agents on storage and purification of proteins or vaccines; drugs and gene deliver agents; antifreeze agents.

The method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation belongs to preparation of supplementary exoenzyme used for marsh gas fermentation. The invention is to provide a method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation, which has the production bacteria with the following enzymatic vitality index: selecting wheat bran and rice bran as mixed culture medium, obtaining mixed bran seeds by pedigree breed two-stage enlargement culture of the bacteria with solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic vitality index, adding commodity enzyme preparation to reach enzymatic vitality index, and airing until the humidity is lower than 5%. The enzymatic vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug tyrosine / g / min. The bacteria can be obtained by bacteria and fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the methane tank fermentation raw materials, the rate of dissolution and degradation of the marsh gas fermentation raw materials can be improve, and the gas-producing rate of marsh gas fermentation, TS and VS gas-production potential and methane content in marsh gas can be increased.

The invention relates to a method for preparing scallop hydrolyzed protein powder, in particular to a method for preparing scallop hydrolyzed protein powder by using scallop scrap wastes, which belongs to the field of resource environment. The method uses the fresh or frozen scallop scrap wastes as a raw material and comprises the following steps: firstly, washing the raw material after the scallop scrap waste are washed or unfrozen, mashing into meat paste by a chopper mixer; secondly, grinding the mashed raw material into colloid homogenate by a colloid mill, and processing the homogenate by the ultrasonic waves; and thirdly, adding an exogenous enzyme to be conducted for enzymolysis, filtrating, sterilizing, condensing and drying a hydrolysate to obtain the scallop hydrolyzed protein powder. With the adoption of the ultrasonic waves, the lysate and endoenzyme can be effectively promoted to be released, and the use ratio of the exogenous enzyme can be improved; furthermore, the product has trophism and functionality; and meanwhile, the protein powder is fresh in taste, high in contents of protein and amino nitrogen and implements green and environmental protection, so that the protein powder can be served as an ingredient of the condiments including seafood soy sauce and seafood seasoning packet, and can be served as the raw material for health food and medicine deep processing.

The invention discloses a method for preparing super-aroma grease by controlling hydrolysis and hot reaction, which comprises the following steps: grinding kernels after shelling (removing red membranes for peanuts) of an oil material into a pulp; stirring and reacting the pulp for certain time at certain temperature and pH; adding an amount of salad oil; transferring the mixture into a high-pressure reaction kettle to react for certain time at certain temperature; and centrifugating the obtained reaction mixture and then pumping out an upper layer oil phase which is a super-aroma grease product. The method has the characteristics of without adding exogenous enzymes or other additives, having strong and pure product aroma and the like. The method is applicable to preparing the super-aroma grease corresponding to various oil materials.

The invention discloses a preparation method of a buffalo milk pickled ripened cheese. The preparation method comprises the following steps of: standardizing buffalo milk without sterilization or pasteurization, adding a lactic acid leavening agent, calcium chloride and chymosin, curdling the milk, cutting, stirring, heating, adding neutral protease, flavourzyme, lipase and other exogenous enzymes into curdled milk particles after whey is discharged, filling, squeezing and demoulding, then pickling in high-concentration saline water, airing, carrying out vacuum package by using a polyethylene or polyvinylidene chloride packaging bag and ripening to obtain a finished product. All indexes of the buffalo milk pickled ripened cheese obtained by using the method are up to the standard of a hard or superhard whole milk ripened cheese, the ripening time is shortened to be within 3-6 months, the buffalo milk pickled ripened cheese can be stored for 180 days at 2-4 DEG C; the product has strong flavor of the whole milk ripened cheese and no poor flavor and is uniform and fine in texture.

The invention relates to a processing method for improving the quality of a tobacco stem extract, comprising the following steps: adding an exogenous enzyme into the tobacco stem extract to carry out enzymolysis; filtering and concentrating the tobacco stem extract subjected to enzymolysis; adding an ammonium salt into the concentrated tobacco stem extract, and fully dissolving the ammonium salt. Because the exogenous enzyme is added into the exogenous enzyme to carry out enzymolysis, the large-molecule compound in the tobacco stem extract, which is not good for the smoking quality, is degraded into the beneficial small molecular substances. The ammonium salt added into the concentrated tobacco stem extract can be subjected to Maillard reaction with the saccharides in the tobacco stem extract during the combustion of the cigarette. The concentrated solution can be used for the cut tobacco stem to improve the quality of the cut tobacco stem significantly and improve the utilization rate of the cut tobacco stem in the cigarette.

The invention discloses a method for increasing fragrance of large leaf green tea, and belongs to the tea processing field. The method adds an external enzyme in a processing process of the large leaf green tea, and enhances enzymatic decomposition of a precursor substance of the fragrance to increase the content of aromatic substances in finished product tea and improve the comprehensive quality of summer tea. The method is simple, has low production cost, has an obvious effect for increasing the fragrance of the finished product tea and the value of the tea leaf, and has no chemical pollutions.

The invention belongs to the field of deep processing of aquatic products and biotechnology and particularly relates to a method for preparing ACE (angiotensin converting enzyme) inhibitory peptide of a protein source of aloididae aloidis by utilizing an endogenous enzyme. The endogenous enzyme in the aloididae aloidis is enzymatically hydrolyzed and autolyzed to obtain natural ACE peptide; no exogenous enzyme is added in a preparation process; the whole preparation process comprises the steps of preprocessing fresh aloididae aloidis, homogenizing, activating the endogenous enzyme, performing enzymolysis, performing enzyme deactivation, deodorizing, performing centrifuging, and performing freeze-drying. The method not only solves an important bottleneck problem existent in a basic research involved in constraints on extension and upgrade of a shellfish industry chain but also opens up new high-quality protein resources.

The invention relates to the field of biotechnologies, and particularly discloses a fusion expression and purification method for recombinant proteins by the aid of alkaline tags and intein and application of the fusion expression and purification method. The fusion expression and purification method and the application have the advantages that fusion expression can be carried out on the to-be-studied target proteins, the intein with a self-splicing function and the alkaline tags, and accordingly the target proteins can be quickly purified by means of ion exchange chromatography; exogenous enzymes can be omitted by the fusion expression and purification method as compared with the traditional methods, the ion exchange cost is low, and accordingly the fusion expression and purification method has an excellent application prospect in industrial production on enlarged scales.