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Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds

A tea seed polysaccharide, comprehensive extraction technology, applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems that did not take into account the industrial production of tea seed polysaccharide, and achieve the effect of low cost and simple extraction equipment

Inactive Publication Date: 2011-01-12
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extraction of polysaccharides from tea seed meal and camellia oleifera seed meal, although there are a few reports in the literature, they mainly focus on the activity research after extraction of polysaccharides, without considering the industrial production of tea seed polysaccharides

Method used

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  • Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds
  • Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Select high-quality Camellia oleifera seeds for shelling and peeling, crush them to 60-80 meshes, treat them with atmospheric pressure steam for 20 minutes, and make a citric acid-sodium citrate buffer solution with pH=5.5 at a solid-liquid ratio of 1:6, at 55°C Add 0.5% of 1:1 cellulase and Neutrase 1.5MG neutral protease under the conditions of the enzymolysis for 6 hours, and keep stirring. Inactivate the enzyme in a water bath at 90°C for 10 min. Centrifuge at 3500r / min to obtain the first-level clear oil layer, emulsified oil layer, water layer and slag layer, heat the emulsified oil layer in a water bath at 70°C for 20 minutes to break the emulsion, take the second-level free clear oil, and combine the two oil layers to obtain crude tea seed oil. The slag layer can be subjected to secondary water extraction, the extraction solid-liquid ratio is 1:4, the extraction temperature is 70°C, and the extraction time is 1.0h. After filtering to remove the slag, the water l...

Embodiment 2

[0043] Select high-quality Camellia oleifera seeds for shelling and peeling, crush them to a size greater than 80 mesh, and treat them with atmospheric pressure steam for 20 minutes, then prepare a citric acid-sodium citrate buffer solution with a solid-to-liquid ratio of 1:5 at a temperature of 55°C. Add 0.5% Neutrase 1.5MG neutral protease under the condition, enzymatic hydrolysis for 6h, and keep stirring. Inactivate the enzyme in a water bath at 90°C for 10 minutes. Centrifuge at 3500r / min. After centrifugation, the system is divided into: first-level clear oil layer, emulsified oil layer, water layer and slag layer. The emulsified oil layer is centrifuged to break the emulsion, and the second-level free clear oil can be taken. The two-stage oil layers are combined to obtain Crude tea seed oil with a yield of 25.8%; the water layer was precipitated with 75% ethanol, and the obtained precipitate was dissolved in water and dried to obtain tea seed polysaccharides with a puri...

Embodiment 3

[0045] After shelling and peeling selected high-quality tea seeds, crush them to 60-80 meshes, treat them with atmospheric pressure steam for 25 minutes, and make a citric acid-sodium citrate buffer solution with pH=4 at a solid-liquid ratio of 1:5, at 55°C Add 0.5% BAN medium-temperature amylase under certain conditions, and perform enzymatic hydrolysis for 6 hours with constant stirring. Inactivate the enzyme in a water bath at 90°C for 10 min. Centrifuge at 3500r / min to obtain the first-level clear oil layer, emulsified oil layer, water layer and slag layer, heat the emulsified oil layer in a water bath at 70°C for 20 minutes to break the emulsion, take the second-level free clear oil, and combine the two oil layers to obtain crude tea seed oil. The extraction rate is 25.3%; the slag layer can be subjected to secondary water extraction, the extraction solid-liquid ratio is 1:4, the extraction temperature is 70°C, and the extraction time is 1.0h. After filtering to remove th...

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Abstract

The invention discloses a comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds, belonging to the process fields of grain and oil processing and byproduct extraction. The method comprises the following steps of: with shelled tea seeds as a raw material, firstly, cooking to produce oil; then, adding an exogenous enzyme to obtain a boiled oil layer of a first level an emulsified oil layer, a water layer and a slag layer through zymolysis, blanching and still standing; demulsifying the emulsified oil layer to take boiled oil of a second level; mixing the oil layers of the two levels; centrifugalizing to obtain the tea seed oil; depositing the water layer in alcohol to separate the tea saponin and the tea seed polysaccharide; leaching the slag layer with water or monohydric alcohol to further separate the tea saponin and the tea seed polysaccharide; and purifying a clear solution which is leached with the water or the alcohol with a macroporous absorption resin chromatography column, eluting a resin adsorbate with a base and then an ethanol and collecting an ethanol eluant to obtain high-purity tea saponin. The tea seed oil, the tea saponin and the tea seed polysaccharide are synchronously prepared, thus the invention realizes that a tea seed or camellia seed resource is comprehensively extracted and utilized and has the advantages of simple process, less needed equipment and low cost.

Description

technical field [0001] The invention belongs to the fields of grain and oil processing and deep processing of agricultural by-products, and specifically relates to a comprehensive extraction method for re-extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia oleifera seeds. Background technique [0002] Tea seeds are the fruit of the tea tree, a by-product of tea production. Mature tea seeds are composed of 23% to 27% fruit shell, 23% to 27% seed shell and 43% to 47% seed kernel, of which the seed kernel contains about 35% fat, about 20% starch, about 11% protein, saponin About 12%, lignin about 11%, ash about 2%. For a long time, people have paid more attention to the planting and processing of tea and the development of tea products, but less attention has been paid to the development and utilization of tea seeds. Therefore, except for the tea tree breeding, most of the mature tea seeds fall off naturally and rot in the ground, resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C07J63/00C07H15/256C07H1/08C08B37/00
Inventor 魏新林王元凤郭艳红席雄刚
Owner SHANGHAI NORMAL UNIVERSITY
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