The method for preparation of hydrolytic
enzyme preparation used for improving gas-producing rate of
marsh gas
fermentation belongs to preparation of supplementary
exoenzyme used for
marsh gas
fermentation. The invention is to provide a method for preparation of hydrolytic
enzyme preparation used for improving gas-producing rate of
marsh gas
fermentation, which has the production
bacteria with the following enzymatic
vitality index: selecting wheat
bran and rice
bran as
mixed culture medium, obtaining mixed
bran seeds by pedigree
breed two-stage enlargement culture of the
bacteria with
solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic
vitality index, adding commodity
enzyme preparation to reach enzymatic
vitality index, and airing until the
humidity is lower than 5%. The enzymatic
vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug
tyrosine / g / min. The
bacteria can be obtained by bacteria and
fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the
methane tank fermentation raw materials, the rate of
dissolution and degradation of the
marsh gas fermentation raw materials can be improve, and the gas-producing rate of
marsh gas fermentation, TS and VS gas-production potential and
methane content in
marsh gas can be increased.