Method for rapidly determining vitality of rice stigma

A technology for rapid determination of rice, applied in the field of rice physiology, can solve the problems of difficult preservation, difficulty in systematic research on stigma vitality, and small size of rice stigma.

Inactive Publication Date: 2021-10-08
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to too many factors affecting stigma vitality, light, temperature, humidity, carbon dioxide content, etc. will cause subtle changes in stigma vitality, and rice stigmas are small in size and difficult to preserve, which brings great challenges to the systematic study of stigma vitality. many difficulties

Method used

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  • Method for rapidly determining vitality of rice stigma
  • Method for rapidly determining vitality of rice stigma
  • Method for rapidly determining vitality of rice stigma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Seed setting rate method measures stigma vigor

[0025] Specific steps are as follows:

[0026] 1. Ear selection for sterile lines: 1 day before pollination, ear selection for sterile lines is carried out, and individual plants with multiple ears and only a few flowers in vigorous and consistent growth conditions are selected, and numbered and listed for marking.

[0027] 2. CMS panicle whole: cut off the spikelets that have bloomed and the spikelets that have not been drawn out, cut off half of the glumes on the spikelets that are suitable for growth, and seal the spikes with kraft paper bags to prevent interference from external pollen.

[0028] 3. Pollination: Half an hour before pollination, select the main ear that is about to bloom in the parent field, take it off completely, immerse it in water for a short time, and then hit it evenly with medium force, sandwich it in dry and warm newspaper for storage, open the kraft paper bag after flowering, and p...

Embodiment 2

[0036] Embodiment 2 Hydrogen peroxide method measures stigma activity

[0037] Specific steps are as follows:

[0038] 1. Ear selection for sterile lines: 1 day before pollination, ear selection for sterile lines is carried out, and individual plants with multiple ears and only a few flowers in vigorous and consistent growth conditions are selected, and numbered and listed for marking.

[0039] 2. Prepare a hydrogen peroxide solution with a volume fraction of 3%, and store it in a refrigerator at 4°C.

[0040] 3. CMS flower selection: Select 50 stigma-exposed florets with the same growth process and flowering on the same day from the main spikes selected for each material, and mark them on the glumes with a red marker.

[0041] 3. Pollination: Half an hour before pollination, select the main ear that is about to bloom in the parent field, take it off completely, immerse it in water for a short time, and then hit it evenly with medium force, sandwich it in dry and warm newspap...

Embodiment 3

[0050] The correlation analysis of embodiment 3 two kinds of methods

[0051] In order to verify the correlation of the data obtained by the two methods, the CORREL function correlation analysis was carried out on the two sets of data, and the results are shown in Table 3.

[0052] Table 3 Correlation analysis between seed setting rate and stigma vigor index

[0053]

[0054]

[0055] Note: **: 0.01 significant level; *: 0.05 significant level.

[0056] As shown in Table 3, classification by material: Among the 6 materials, the correlation of the data measured by the two groups of methods is greater than 0.97, which is a very significant positive correlation; no distinction is made between materials, classification by method: correlation analysis is performed on all data to obtain The total correlation coefficient of is 0.918641, a very significant positive correlation. In conclusion, the results of determination of stigma viability by hydrogen peroxide method are clos...

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Abstract

The invention discloses a method for rapidly measuring vitality of rice stigma, which comprises the following steps: (1) taking pollinated glumous flowers after flowering of a rice plant to be measured, taking out stigmas from the glumous flowers, removing styluses, placing on a glass slide containing a hydrogen peroxide aqueous solution with the volume fraction of 1-10%, and covering with a cover glass; and (2) placing the glass slide under the 4 * view of the optical microscope, starting timing, counting the time when the bubbles in the hydrogen peroxide aqueous solution expand to fill the whole 4 * view screen of the optical microscope, and obtaining the duration which is the rice stigma vitality index. According to the method, the vitality of the rice stigma is detected on the basis of catalase activity, and the obtained stigma vitality index is remarkably positively correlated with the maturing rate obtained by a traditional maturing rate method, so that the method can effectively reflect the rice stigma vitality; and the method is hopeful to play an important role in cultivation of a sterile line with high stigma vitality and research and development of a production technology system for improving the stigma vitality.

Description

technical field [0001] The invention relates to the technical field of rice physiology, in particular to a method for quickly measuring rice stigma vigor. Background technique [0002] Stigma vitality refers to the ability of stigma to accept pollen and carry out normal fertilization after flowering. In rice production, stigma vigor determines the length of time for receiving pollen and pollination efficiency after the glume closes, and it can affect the yield of seed production together with the stigma exposure rate. [0003] There is a direct relationship between stigma activity and seed setting rate. Tian Dacheng et al. (Tian Dacheng, Huang Sankui, Duan Yongguo, etc.. The relationship between flowering time and pollination time of rice male sterile line and outcross seed setting rate[J]. Hybrid Rice, 2004, 3:53 -57) The study found that the exposed stigma florets after flowering can still combine pollen, and the number of seeds is about one-half of the total number. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84
CPCG01N21/84G01N2021/8466
Inventor 刘振宇汪庆舒庆尧
Owner ZHEJIANG UNIV
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