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Application of Citrulline in Improving Plant Seed Tolerance to Salt Stress

A plant seed, citrulline technology, applied in the application, seed immunization, seed and rhizome treatment and other directions, can solve the problems affecting watermelon facility cultivation, yield and quality, affecting the growth and development of watermelon seeds, etc., to improve the germination rate. , high yield and quality, the effect of reducing absorption

Active Publication Date: 2020-06-23
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salt stress has become a major problem affecting watermelon protected cultivation, seriously affecting the growth and development of watermelon seeds, and having a great impact on yield and quality

Method used

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  • Application of Citrulline in Improving Plant Seed Tolerance to Salt Stress
  • Application of Citrulline in Improving Plant Seed Tolerance to Salt Stress
  • Application of Citrulline in Improving Plant Seed Tolerance to Salt Stress

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Experimental program
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Effect test

Embodiment 1

[0018] The application of citrulline in improving the tolerance of plant seeds to salt stress, the application method is to apply citrulline to watermelon seeds subjected to salt stress, the application concentration of the citrulline is 0.1mmol / L, the citrulline The acid application rate is 1 mL per 50 plant seeds; the salt stress environment is that the NaCl concentration is 130 mmol / L.

[0019] The application method includes the following steps:

[0020] (1) Select mature and plump plant seeds, first disinfect them with 0.25% potassium permanganate solution for 10 minutes, and then rinse the plant seeds with sterile water.

[0021] (2) Soak the plant seeds treated in step (1) with the citrulline solution for 4 hours under salt stress, then place the plant seeds in a petri dish covered with filter paper, and place the petri dish at a constant temperature of 28°C Cultivated in a box.

[0022] (3) Apply the citrulline solution to the culture dish, replace it every 22 hours,...

Embodiment 2

[0024] The application of citrulline in improving the tolerance of plant seeds to salt stress, the application method is to apply citrulline to watermelon seeds subjected to salt stress, the application concentration of citrulline is 0.8mmol / L, and the citrulline The acid application rate is 3 mL per 50 plant seeds; the salt stress environment is that the NaCl concentration is 140 mmol / L.

[0025] The application method includes the following steps:

[0026] (1) Select mature and plump plant seeds, first disinfect them with 0.35% potassium permanganate solution for 15 minutes, and then rinse the plant seeds with sterile water.

[0027] (2) Soak the plant seeds treated in step (1) with the citrulline solution for 6 hours under salt stress, then place the plant seeds in a petri dish covered with filter paper, and place the petri dish at a constant temperature of 32°C Cultivated in a box.

[0028] (3) Apply the citrulline solution to the culture dish, replace it every 28 hours,...

Embodiment 3

[0030] The application of citrulline in improving the tolerance of plant seeds to salt stress, the application method is to apply citrulline to watermelon seeds subjected to salt stress, the application concentration of citrulline is 0.4mmol / L, and the citrulline The acid application rate is 2 mL per 50 plant seeds; the salt stress environment is that the NaCl concentration is 136.2 mmol / L.

[0031] The application method includes the following steps:

[0032] (1) Select mature and plump plant seeds, first disinfect them with 0.3% potassium permanganate solution for 12 minutes, and then rinse the plant seeds with sterile water.

[0033] (2) Soak the plant seeds treated in step (1) with the citrulline solution for 5 hours under salt stress, then place the plant seeds in a petri dish covered with filter paper, and place the petri dish at a constant temperature of 30°C Cultivated in a box.

[0034] (3) Apply the citrulline solution to the culture dish, replace it every 24 hours...

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Abstract

The invention provides application of citrulline in improvement of salt stress resistance of plant seeds. When the citrulline is applied to the plant seeds subjected to salt stress, the germination rate, germination potential, germination index, vitality index, root activity, radicle length and hypocotyl length of the plant seeds subjected to salt stress can be improved, and the seed germination is promoted, so that the effects of enabling the plant seeds to be high in survival rate, yield and quality are achieved.

Description

technical field [0001] The invention relates to the application of citrulline in improving plant seeds tolerance to salt stress. Background technique [0002] In recent years, the problem of soil salinization has become increasingly prominent, and the threat to agriculture is a global problem. With the expansion of industrial modernization, irrigated land and plastic greenhouses, soil secondary salinization has become increasingly serious, so crops are resistant to The improvement of salinity is one of the major agricultural researches. Salt stress is one of the important environmental factors that inhibit plant growth and reduce crop yield. Watermelon is one of the main crops in my country and occupies an important position in the world's horticultural industry. Salt stress causes damage to plants in many ways, including osmotic stress, ion poisoning, and destruction of intracellular ion balance, which inhibits plant growth. Photosynthesis decreases, energy consumption incr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 贺滉詹园凤党选民李嘉杜公福牛玉
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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