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Index combination, kit and method for evaluating human immune function

An immune function, human body technology, applied in biochemical equipment and methods, biological testing, individual particle analysis, etc., can solve problems that cannot be used as a deterministic diagnosis basis, and achieve a comprehensive evaluation effect.

Pending Publication Date: 2022-07-26
广州正源生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some patients have no perceivable symptoms, and even the detection of tumor markers cannot be used as a definitive diagnosis due to individual differences

Method used

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  • Index combination, kit and method for evaluating human immune function
  • Index combination, kit and method for evaluating human immune function
  • Index combination, kit and method for evaluating human immune function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Example 1 Immune cell atlas analysis

[0113] 1. CS&T research Beads quality control

[0114] The flow cytometer is a BD FACSAria IIU. First add 500 μL of PBS to the flow tube, then add 3 drops of CS&T research Beads that are mixed upside down and vortexed for 10 seconds;

[0115] Open the Diva software, select Cytometer→CS&T→select the corresponding lot, select "checkpreformance", and click "Run";

[0116] After the test is completed, after the report shows "Successfully", a CS&T test report is issued, the CS&T system is closed, and the samples are ready to be tested.

[0117] 2. Reagent preparation

[0118] Hemolysin (working solution): Take BD 10×FACS lysing Solution, add deionized water and dilute 10 times. That is, 1 mL of BD 10×FACS lysing Solution was added to 9 mL of deionized water, mixed well, and prepared as a hemolysin working solution.

[0119] 3. Sample preparation

[0120] 1. Take 1 mL of whole blood, dispense 100 μL / branch into flow tubes, add the cor...

Embodiment 2

[0151] Example 2 Analysis of proliferation ability and viability of immune cells

[0152] The normal immune cell morphology is: the cell body is 12-20 μm in diameter, round or irregular in shape, and translucent; the nucleus is irregular in shape and has obvious twisted and folded; the nuclear chromatin is fine, loose in the shape of a wire mesh or cord; The mass is high, and fine, uniformly dispersed dust-like particles can be seen; colonies are formed after culture.

[0153] 1. Detection method of the proliferation ability of immune cells

[0154] D0 ready cells

[0155] 1. Isolate PBMC and stimulate overnight with factor

[0156] (1) Collect 25ml of blood from a heparin anticoagulant tube and shake gently. Balanced, centrifuged at 400 × g for 10 min.

[0157] (2) Use a pipette to suck the upper plasma into a new centrifuge tube, inactivate at 56°C for 30 minutes, then centrifuge at 1000×g for 15 minutes at high speed, collect the upper layer of serum and a new centrifug...

Embodiment 3

[0196] Example 3 Analysis of killing ability of immune cells

[0197] D0: Isolated PBMCs were stimulated with factors overnight

[0198] (1) Collect 25ml of blood from a heparin anticoagulant tube and shake gently. Balanced, centrifuged at 400 × g for 10 min.

[0199] (2) Use a pipette to suck the upper plasma into a new centrifuge tube, inactivate at 56°C for 30 minutes, then centrifuge at 1000×g for 15 minutes at high speed, collect the upper layer of serum and a new centrifuge tube, and store at 4°C for later use.

[0200] (3) The blood cells in the middle and lower layers of "(1)" were diluted 1:1 with normal saline, gently added to the lymphocyte separation medium from the edge of the centrifuge tube (blood: ficoll=2:1), and centrifuged at room temperature (680×g, 20min) ), suck the buffy coat cells into a centrifuge tube, wash 3 times with NS or PBS, centrifuge, and the 3 times centrifugation conditions are 1800r / min, 5min;

[0201] (4) The above-mentioned PBMCs were ...

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Abstract

According to the index combination for evaluating the human immune function, the kit and the method provided by the invention, the number index of immune cells, the multiplication capacity and vitality index of the immune cells and the killing capacity index of the immune cells are combined for evaluating the state of the human immune function for the first time; the evaluation effect of the index combination is more comprehensive and more accurate, the killing ability and the anti-escape ability of the human immune cells to the cancer cells can be comprehensively evaluated, and then the zero clearing ability of the cancer cells can be accurately monitored.

Description

technical field [0001] The present application relates to the field of medical care, and in particular, to a combination of indicators, a kit and a method for evaluating human immune function. Background technique [0002] Early detection, early treatment, and early cure of cancer have become a consensus, but most of the existing methods for detecting and evaluating cancer focus on the detection of cancer cells, and there are few immune function evaluations for the human body against cancer. With the development of my country's economy and the improvement of people's living standards, the investment in health care has also increased. Gene sequencing, multi-slice CT, PET, tumor marker microfluidic chip and ctDNA detection are gradually appearing in the service items of some high-end medical institutions. It is well known that although tumors and some chronic diseases are genetically related, genetic factors are not the only decisive factor. However, some patients have no pe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N33/569G01N33/58C12Q1/02
CPCG01N15/14G01N33/56966G01N33/5011G01N33/5047G01N33/582
Inventor 高东谢佳琦卢瑞珊甘丽晶金建秋
Owner 广州正源生物技术有限公司
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