The invention discloses a method for establishing the HPLC fingerprint of a Yinzhihuang granule. The method comprises the following steps: preparing a sample solution, determining HPLC chromatographicconditions, and making an HPLC standard fingerprint. The invention also discloses a the HPLC standard fingerprint of the Yinzhihuang granule, obtained through the method. The fingerprint has twenty-nine common peaks, and the relative retention times tR of the common peaks sequentially respectively are 0.079, 0.087, 0.113, 0.341, 0.436, 0.563, 0.648, 0.866, 0.891, 0.905, 0.919, 1.000, 1.089, 1.129, 1.161, 1.197, 1.395, 1.453, 1.496, 1.597, 1.776, 1.808, 1.979, 2.071, 2.136, 2.168, 2.353, 2.396 and 2.440. The method has the advantages of simplicity in operation, high stability, good reproducibility, a large quantity of characteristic peaks in the obtained fingerprint, realization of comprehensive evaluation of the quantity of the Yinzhihuang granule through comparing the common peaks in thestandard fingerprint, facilitation of the stabilization of the product quality, and ensuring the clinical medication safety and effectiveness.