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Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis

A quality control method and technology of Cordyceps fungus powder, which can be applied to plant raw materials, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve problems such as not seen, and achieve simple operation, strong specificity, and good repeatability. Effect

Active Publication Date: 2008-10-29
SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the analysis of the fingerprint of Cordyceps sinensis by high performance liquid chromatography in the herbal preparation of Fuzheng Huayu.

Method used

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  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis
  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis
  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Fingerprint test method

[0060] 1. Reagents and medicinal materials

[0061] Purified water (Shui Sen live, Coca-Cola Company); Acetonitrile (chromatographically pure, Tedia, the United States); Adenosine (China Pharmaceutical and Biological Products Identification Institute, for content determination, batch number: 879-200202); Uridine (China Pharmaceutical and Biological Products Identification Institute, for identification, batch number: 887-200202); guanosine (purchased from Xinxiang Tuosheng Chemical Technology Co., Ltd.); Cordyceps mycelium powder (produced by the present invention).

[0062] 2 experimental equipment

[0063] HP1100 Liquid Chromatograph (DAD Diode Array Detector, Agilent, USA); Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd., KQ-50E); Chromatographic Column Thermostat (Tianjin Hengao Technology Development Co., Ltd., HT-130 ); HPLC membrane (Jiangsu Hanbang Technology Co., Ltd., 0.45um)

[0064] 3 Extraction of Cordyceps mycelium p...

Embodiment 2

[0071] Take 10mL primary seeds into a 500mL Erlenmeyer flask containing 100mL secondary seed medium, rotating speed: 150rpm, pH6.2-6.8; cultivate under aerobic conditions at 28°C for 2-5 days; Strain, to start large-scale Cordyceps fermentation with fermenter; use fermentation medium in fermenter, rotating speed 150-250rpm; cultivate at 28°C dissolved oxygen concentration: 20-90 for 2-5 days, pH 6.2-6.8; The culture medium is removed, and the mycelium is dried to obtain the product Cordyceps fungus powder.

[0072] The seed medium is: glucose 2%, peptone 1%, ammonium nitrate 0.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, ferrous sulfate 0.001%, sodium chloride 0.1%, bran 5%;

[0073] The fermentation medium is: glucose 2%, peptone 1%, ammonium nitrate 0.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, ferrous sulfate 0.001%, sodium chloride 0.1%, 5% silkworm chrysalis powder 5%;

[0074] Fermentation medium, 5% bran, 5% silkworm chrysalis...

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Abstract

The invention relates to a control method of the fingerprint spectrum quality of cordyceps sinensis powder raw material in botanical drug for strengthening vital qi and removing blood stasis, comprising the steps that: (1) cordyceps sinensis powder is extracted: 0.100g of cordyceps sinensis powder is taken, purified water is added, the ultrasonic extraction, the filtration and the sample injection are carried out; (2) the gradient elution with mobile phase is carried out: octadecyl silane bonded silica gel is taken as a filler, water and acetonitrile are taken as mobile phase to carry out the gradient elution for 0 to 30min and 0 to 7 percent B; (3) a standard fingerprint spectrum is established: the HPLC standard fingerprint spectrum of the cordyceps sinensis powder is determined, and 3 characteristic peaks are selected; (4) the quality control of the fingerprint spectrum is carried out: the relative retention time of No.2 peak uridine, No.3 peak guanosine and No.4 peak adenosine are 0.44 plus or minus 0.03, 0.68 plus or minus 0.03 and 1.00 respectively; the HPLC fingerprint spectrum of the sample is compared with the contrast fingerprint spectrum. The similarity calculated by the 5 common peaks is not less than 0.9, (5) the preparation of the cordyceps sinensis powder raw material is carried out; the control method has good repetitivity and can fully reflect the basic characteristics of nucleoside ingredients of the cordyceps sinensis powder.

Description

technical field [0001] The invention belongs to the field of quality control methods for raw materials of Cordyceps fungus powder, and in particular relates to a method for quality control of fingerprints of raw materials of Cordyceps fungus powder in herbal medicines for strengthening health and removing blood stasis. Background technique [0002] Cordyceps sinensis is a compound of the subunits and larvae corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of bat moths. It is a famous and precious traditional Chinese medicine for a long time. It is distributed in Qinghai, Tibet, Sichuan and other places in China, and exists in grassy soils in mountainous areas at an altitude of 3000-4000m in Northwest and Southwest China. The part exposed on the soil surface is the fruiting body of the fungus, 3-6cm long, black in color, and shaped like a blade. The body part buried in the soil is 2.5-4m long, yellow to dark brown. Because its shape changes uniquely...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P15/10A61P11/00A61P29/00G01N30/02G01N30/86C12N1/14
Inventor 胡坪潘一峰
Owner SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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