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Method for judging aged rice seeds based on glycerin content change ratio

A rice seed and glycerin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of many factors of data changes, time-consuming and labor-intensive, etc., to overcome time-consuming and labor-intensive, save seeds, and improve discrimination efficiency Effect

Pending Publication Date: 2020-10-16
HUNAN HYBRID RICE RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For a long time, the identification of old and new seeds has been mainly carried out by the determination of germination rate, but the determination of seed germination rate is time-consuming and labor-intensive, and second, there are many factors that affect data changes, such as the temperature at the time of measurement and the water absorption of seeds. How much to wait

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  • Method for judging aged rice seeds based on glycerin content change ratio
  • Method for judging aged rice seeds based on glycerin content change ratio
  • Method for judging aged rice seeds based on glycerin content change ratio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Four hybrid rice combinations including B3Y998, IIY998, TY998 and QY998 were selected as experimental materials, and the harvested seeds were subjected to natural aging treatment for 2 years.

[0048] Afterwards, a certain amount of above-mentioned rice seeds is taken, and its germination rate is measured according to the "Method for Determination of Germination Rate of Rice Seeds" (GB / T 3543); at the same time, the seed metabolite glycerin is measured according to the above-mentioned extraction of glycerol and the quantitative determination method of relative content. The extraction and quantitative determination of rice seeds before and after storage were calculated and recorded as K (Table 1). The content of glycerol in the seed metabolite refers to the average content of glycerol in the whole seed, that is, the content of the mixture of embryo and endosperm in the seed content.

[0049] It can be seen from Table 1 that after 2 years of natural aging of all the hybri...

Embodiment 2

[0057] A certain number of Guang 8 You 169 seeds were taken, artificially aged to simulate the naturally aged seeds, and the glycerol content and germination rate in the endosperm of the seeds after different aging times were measured. As can be seen from Table 2, the germination rate of seeds after artificial aging drops sharply (the lower the germination rate of artificial aging seeds, the lower the seed vigor); According to the regulation that the germination rate of qualified hybrid seeds is not less than 80%, they are all unqualified seeds. And the content of glycerol in the endosperm increases along with the aging time on the whole, and the lowest K value of the glycerol content before and after storage of the varieties (combinations) done in this experiment is 2.45 (this value is greater than 2.32), and the germination rate is only 55.5%. For low vigor old seeds. This example can illustrate that the present invention uses the glycerol content in the seed endosperm as t...

Embodiment 3

[0064] In the natural storage of rice seeds, the old seeds of three varieties (combinations) of Nanyouzhan, Guang8B and Guanghui 308 after natural storage for a certain period of time and the same new seeds of the above three kinds (not stored) were selected as follows: Material, get a certain amount of above-mentioned rice seed afterwards and carry out experiment.

[0065] The first step: determine the absolute content of glycerol in the seeds and calculate the K value (Table 3). The absolute content of the seed metabolite glycerol was determined according to the above glycerol extraction and quantitative methods, and the ratio of glycerol in rice seeds before and after storage was calculated. See Table 3 for specific data.

[0066]The second step: Discriminate the seed vigor according to the K value. Since the K values ​​of Guang 8B and Guanghui 308 in Table 3 were both greater than 2.32, we judged that the above two combined seeds belonged to low vigor seeds. However, th...

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Abstract

The invention belongs to the field of rice seeds, and discloses a method for judging aged rice seeds based on a glycerin content change ratio. The method comprises the following steps of extracting glycerol in rice seeds, and quantitatively determining the content of glycerol in the rice seeds, measuring the glycerol content in the new rice seeds by adopting the method to serve as a reference vitality index of the rice seeds, measuring the glycerol content of the rice seeds which are naturally aged for two years by adopting the method, determining the content of glycerol in the rice seeds of which the aged seeds need to be judged by adopting the method, calculating the ratio K of the glycerol content in the rice seeds of which the aged seeds need to be judged to the reference vitality index of the rice seeds, measuring the ratio of the glycerol content in the rice seeds after two years of natural aging to the reference vitality index of the rice seeds, and recording the ratio as K1, judging whether the stored rice seeds belong to aged seeds or not according to whether the K value is larger than or equal to K1 or not, and judging that the stored rice seeds belong to the aged seeds when the K value is larger than or equal to K1.

Description

technical field [0001] The invention belongs to the field of rice seeds, in particular to a method for discriminating old rice seeds based on the glycerol content variation ratio. Background technique [0002] Rice seed is a special means of agricultural production, and it is a prerequisite for the normal development and production of rice. With the change of sowing methods and the frequent occurrence of extreme weather, the requirements of agricultural production on the quality of seed sowing are increasing year by year. Under natural conditions in the field, especially under unfavorable conditions, keeping seedlings uniform and strong directly determines the production potential and benefits of rice. [0003] Due to the unpredictability of the production of seeds, especially hybrid rice seeds, it is often necessary to store the seeds. During the storage of seeds, there is aging and deterioration, which leads to a serious decline in germination rate, and the germination r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045G01N2030/067
Inventor 马国辉晏石娟刘军魏中伟
Owner HUNAN HYBRID RICE RES CENT
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