Method for judging deteriorated rice seeds based on gluconic acid content change ratio

A technology for gluconic acid and rice seeds, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of many factors of data change, time-consuming and labor-intensive, etc., so as to overcome time-consuming and labor-intensive, save seeds, and improve the discrimination efficiency. Effect

Pending Publication Date: 2020-10-13
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI +1
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Problems solved by technology

[0003] For a long time, the judgment of seed deterioration mainly relies on the determination of germination rate and seedling growth rate. However, the determination of seed germination rate is time-consuming and labor-intensive. Second, there are many factors that affect data changes, such as the temperature at the time of measurement. , the amount of water absorbed by the seeds, etc.

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  • Method for judging deteriorated rice seeds based on gluconic acid content change ratio
  • Method for judging deteriorated rice seeds based on gluconic acid content change ratio

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Experimental program
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Effect test

Embodiment 1

[0045] Hybrid rice combinations such as QY3618, B3Y998, IIY998, and QY998 were selected as experimental materials, and the harvested seeds were naturally aged for 2 years.

[0046] Afterwards, a certain amount of above-mentioned rice seeds is taken, and its germination rate is measured according to the "Method for Determination of Germination Rate of Rice Seeds" (GB / T 3543); at the same time, the extraction of the above-mentioned gluconic acid and the quantitative determination method of the relative content are carried out for the determination of the seed metabolites. The extraction and quantitative determination of gluconic acid calculated the ratio of gluconic acid in rice seeds before and after storage, which is recorded as K1 (Table 1), wherein the content of the seed metabolite gluconic acid refers to the average content of gluconic acid in the whole seed, that is, the The content of the embryo and endosperm mixture.

[0047] It can be seen from Table 1 that after 2 yea...

Embodiment 2

[0057] The 368 series hybrid rice combination was selected as the experimental material, and the harvested seeds were naturally aged for 2 years.

[0058] Get a certain amount of above-mentioned rice seeds afterwards, carry out germination rate measurement to it according to " rice seed germination rate determination method " (GB / T3543); Simultaneously, carry out seed metabolite glucose by the extraction of above-mentioned gluconic acid and the quantitative determination method of relative content. The acid was extracted and quantitatively determined, and the ratio of gluconic acid in rice seeds before and after storage was calculated, which was recorded as K1 (Table 2).

[0059] It can be seen from Table 2 that after 2 years of natural aging of the 368 series hybrid rice seeds tested, the germination rate all decreased. %, for unqualified seeds. Also as can be seen from Table 2, along with the reduction of natural aging seed germination rate, the gluconic acid content in all...

Embodiment 3

[0067] Take Guang 8 You 165, Guang 8B and Guang Hui 308 whose natural aging treatment time is 2 years, and Nanyouzhan whose natural aging treatment time is 1.5 years. The metabolite gluconic acid was extracted and quantitatively measured, and the ratio of gluconic acid in rice seeds before and after storage was calculated, which was recorded as K1: the K1 value of Guang 8 You 165 was 3.156215678, the K1 value of Guang 8B was 3.787893221, and the K1 value of Guanghui 308 was 2.255632878, Nan Youzhan's K1 value is 3.117867776.

[0068] In the natural storage of rice seeds, the old seeds of the combination of Guang 8 You 165, Guang 8B, Guang Hui 308 and Nan You Zhan after a certain period of natural storage and the same four new storage) as the material, and then a certain amount of the above-mentioned rice seeds was taken to carry out the experiment.

[0069] The first step: determine the absolute content of gluconic acid in the seeds and calculate the K value (Table 3). The a...

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Abstract

The invention belongs to the field of rice seeds, and discloses a method for judging deteriorated rice seeds based on a gluconic acid content change ratio. The method comprises the following steps: extracting gluconic acid in rice seeds, and quantitatively determining the content of gluconic acid in the rice seeds; taking the gluconic acid content in the new rice seeds measured by the method as areference vitality index of the rice seeds, and measuring the gluconic acid content in the rice seeds of which the seed vitality needs to be judged; calculating the ratio K of the gluconic acid content in the rice seeds of which the seed vigor needs to be judged to the reference vigor index of the rice seeds; calculating the ratio of the gluconic acid content of the rice seeds which are naturallyaged for 2 years to the reference vitality index of the rice seeds, and recording the ratio as K1; judging whether the stored rice seeds belong to deteriorated seeds or not according to whether the Kvalue is larger than or equal to K1 or not, and judging that the stored rice seeds belong to the deteriorated seeds when the K value is larger than or equal to K1.

Description

technical field [0001] The invention belongs to the field of rice seeds, in particular to a method for discriminating deteriorating rice seeds based on the ratio of changes in gluconic acid content. Background technique [0002] Rice seeds are important agricultural production materials. With the change of sowing methods and the frequent occurrence of extreme weather, the requirements of agricultural production on the quality of seed sowing are increasing year by year. Due to the unpredictability of the production of seeds, especially hybrid rice seeds, it is often necessary to store the seeds. During the storage process, the seeds will inevitably experience an irreversible change process of vigor decline, that is, seed deterioration or aging, resulting in a serious decline in germination rate, emergence rate and production potential, and serious rice production benefits. One of the most common situations in the actual production and application in the south is that rice s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7206G01N30/8679
Inventor 刘军晏石娟高家东张友胜黄文洁
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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