Method for judging low-activity aged rice seeds based on galactose content change ratio

A technology of rice seeds and galactose, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-comparability, different soybeans, expensive nuclear magnetic resonance instruments, etc., to overcome time-consuming and labor-consuming, save seeds, and distinguish simple and fast effect

Pending Publication Date: 2020-10-13
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI +1
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Problems solved by technology

A method for judging soybean seed vigor based on nuclear magnetic resonance technology (application publication number CN 106680307 A) discloses using the absolute content of metabolites in soybean seed extracts to judge seed vigor. The metabolites can be arginine and tryptophan in soybeans. , hypoxanthine, isopropanol, propylene glycol, phosphorylcholine, dimethyl urate, but one or more nuclear magnetic resonance equipment is expensive, and the second is that the metabolites of rice seeds are significantly different from those of soybeans , there is no comparison between the two

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  • Method for judging low-activity aged rice seeds based on galactose content change ratio
  • Method for judging low-activity aged rice seeds based on galactose content change ratio

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Experimental program
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Effect test

Embodiment 1

[0048] B3Y3618, QY3618, QY 998, B3Y998, IIY998 and other hybrid rice combinations were selected as experimental materials, and the harvested seeds were naturally aged for 2 years.

[0049] Afterwards, a certain amount of above-mentioned rice seeds is taken, and its germination rate is measured according to the "Method for Determination of Germination Rate of Rice Seeds" (GB / T 3543); at the same time, the extraction of the above-mentioned galactose and the quantitative determination method of the relative content are carried out for the determination of the seed metabolites. The extraction and quantitative determination of galactose calculated the ratio of galactose in rice seeds before and after storage, which was recorded as K1 (Table 1), wherein the content of the seed metabolite galactose refers to the average content of galactose in the whole seed, that is, the The content of the embryo and endosperm mixture.

[0050] It can be seen from Table 1 that after 2 years of natur...

Embodiment 2

[0063] The 368 series hybrid rice combination was selected as the experimental material, and the harvested seeds were naturally aged for 2 years.

[0064] Afterwards, a certain amount of above-mentioned rice seeds is taken, and its germination rate is measured according to the "Method for Determination of Germination Rate of Rice Seeds" (GB / T 3543); at the same time, the extraction of the above-mentioned galactose and the quantitative determination method of the relative content are carried out for the determination of the seed metabolites. Galactose was extracted and quantitatively determined, and the ratio of galactose in rice seeds before and after storage was calculated, which was recorded as K1 (Table 2).

[0065] It can be seen from Table 2 that after 2 years of natural aging of the 368 series hybrid rice seeds tested, the germination rate all decreased. %, for unqualified seeds. It can also be seen from Table 2 that along with the reduction of the germination rate of n...

Embodiment 3

[0074] Take Guang 8 You 165, Guang 8B and Guang Hui 308 whose natural aging treatment time is 2 years, and carry out the extraction and quantitative determination of the seed metabolite galactose according to the extraction and quantitative determination of the relative content of galactose respectively, and calculate The ratio of galactose in rice seeds before and after storage is recorded as K1: the K1 value of Guang 8 You 165 is 5.278767909, the K1 value of Guang 8B is 8.232156655, and the K1 value of Guanghui 308 is 10.244556355.

[0075] In the natural storage of rice seeds, the old seeds of the combination of Guang 8 You 165, Guang 8B and Guang Hui 308 after natural storage for a certain period of time and the same new seeds of the above three kinds (not stored) were randomly selected as materials. , then take a certain number of the above rice seeds to carry out experiments.

[0076] The first step: determine the absolute content of galactose in the seeds and calculate ...

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Abstract

The invention belongs to the field of rice seeds, and discloses a method for judging low-activity rice seeds based on galactose content change. The method comprises the following steps: extracting galactose in rice seeds, and quantitatively determining the content of galactose in the rice seeds; taking the galactose content in the new rice seeds measured by the method as a reference vitality indexof the rice seeds; using the the method to determine the galactose content in the rice seeds of which the seed vigor needs to be judged; calculating the ratio K of the galactose content in the rice seeds of which the seed vigor needs to be judged to the reference vigor index of the rice seeds; calculating the ratio of the galactose content of the rice seeds naturally aged for 2 years to the reference vitality index of the rice seeds, and recording the ratio as K1; judging whether the stored rice seeds belong to low-activity aged seeds or not according to whether the K value is larger than orequal to K1 or not, and judging that the stored rice seeds belong to aged seeds when the K value is larger than or equal to K1.

Description

technical field [0001] The invention belongs to the field of rice seeds, and in particular relates to a method for discriminating old rice seeds with low vigor based on the change ratio of galactose content. Background technique [0002] Rice seeds are the carrier of genetic material, the prerequisite for the normal development and production of rice, and an important material for long-term preservation of germplasm resources. With the change of sowing methods and the frequent occurrence of extreme weather, the requirements of agricultural production on the quality of seed sowing are increasing year by year. Under natural conditions in the field, especially under unfavorable conditions, keeping seedlings uniform and strong directly determines the production potential and benefits of rice. [0003] Due to the unpredictability of seed production, especially hybrid rice seeds, short-term storage of seeds is often required. Due to the phenomenon of aging and deterioration of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7206G01N30/8679G01N2030/067
Inventor 刘军付华晏石娟高家东黄文洁
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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