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A method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate

A technology of rice seeds and regression equations, applied in germination equipment and other directions, can solve the problems of incomparability, different soybeans, and high cost of nuclear magnetic resonance equipment, so as to improve the discrimination efficiency and save seeds.

Active Publication Date: 2022-02-18
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as a kind of method (application publication number CN106680307 A) based on nuclear magnetic resonance technology to discriminate soybean seed vigor discloses the level of vigor of discriminating seed vigor with the metabolite absolute content in the soybean seed extract, and metabolite can be arginine in soybean, tryptophan, One or more of hypoxanthine, isopropanol, propylene glycol, phosphorylcholine, and dimethyl urate, but one is that nuclear magnetic resonance equipment is expensive and costly, and the other is that the metabolites of rice seeds are significantly different from those of soybeans. no comparison between the two

Method used

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  • A method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate
  • A method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate
  • A method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A certain number of Nanxiuzhan seeds were taken, and seeds with different aging degrees, that is, different germination rates were obtained through natural storage, and the absolute content of galactose in the seeds and the corresponding germination rates were measured respectively (see Table 1 for specific data). The results showed that the absolute content of galactose in seeds tended to increase as the aging time increased; the trend of germination rate was opposite to that of galactose, and the germination rate showed a downward trend with the prolongation of storage time.

[0044] Table 1 Germination rate and galactose content of Nanxiuzhan seeds with different aging degrees

[0045] Varieties with different degrees of aging Germination rate(%) Galactose content (μg / g) Nam Soo Zhan 3 81.33333 50.8096 Nam Soo Zhan 2 81.66667 45.9910 Nam Soo accounted for 1 94 23.80915

Embodiment 2

[0046] Embodiment 2: Take a certain amount of Guang 8 You 165, Guang 8B, and Nan You Ruan Zhan multiple varieties (combination) seeds, after natural storage for different times, measure the absolute content of galactose in the seeds and the corresponding germination rate respectively (see Table 2 for specific data).

[0047] Table 2 The relationship between the germination rate and galactose content of seeds with different aging degrees

[0048]

[0049] With germination rate y, the absolute content of galactose is x, establishes the regression equation of x and y (such as figure 1 mentioned). The comprehensive calculation results of Guang 8 You 165, Guang 8B, Nan You Ruan Zhan and Nan Xiu Zhan show that the regression equation is: y=-0.1881x+89.056, and its coefficient of determination is R 2 = 0.9423. Wherein, y is the germination rate (%), and x is the absolute content of galactose (μg / g).

Embodiment 3

[0051] In the seed storage house, randomly select the seeds of different varieties and different aging degrees to measure the absolute content of galactose, and calculate (predict) the seed germination rate according to the regression equation between the absolute content of galactose and the germination rate obtained in Example 2 ;According to the predicted germination rate, predict whether the seeds are qualified; then according to the "Method for Determining the Germination Rate of Rice Seeds" (GB / T 3543), carry out actual measurement of the seed germination rate and judge whether the seeds are qualified, and finally predict whether the seeds are qualified The results are compared with the actual judgment results. The specific results and conclusions are shown in Table 3.

[0052] Table 3 Judgment of whether the seeds of different aging degrees are qualified

[0053]

[0054] It can be shown from this example that the method of quickly judging whether rice seeds are qua...

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Abstract

The invention belongs to the field of rice seeds and discloses a method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate. The method comprises the steps of: extracting galactose in rice seeds, quantitatively measuring the absolute content of galactose in rice seeds; preparing a batch of seeds with different degrees of aging and deterioration, respectively measuring the absolute content of galactose, measuring the germination rate of the seeds, and calculating and formulating The regression equation between the galactose content and the seed germination rate; the galactose content in the seeds that need to be discriminated is extracted and measured, and the germination rate in the seeds that need to be discriminated is calculated (predicted) according to the regression equation, combined with my country's "Grain Crop Seeds According to the provisions in Part 1 Grains and Cereals, it is necessary to judge the seeds that need to be judged whether they are qualified or not. The invention uses the galactose content as an index for measuring the vigor, which makes the discrimination of the vigor of the seeds simpler, quicker and comparable, improves the discrimination efficiency and saves the seeds.

Description

technical field [0001] The invention belongs to the field of rice seeds, in particular to a method for quickly judging whether rice seeds are qualified by using the regression equation of galactose content and germination rate. Background technique [0002] Rice seeds are the carrier of genetic material, the prerequisite for the normal development and production of rice, and an important material for long-term preservation of germplasm resources. With the change of sowing methods and the frequent occurrence of extreme weather, the requirements of agricultural production on the quality of seed sowing are increasing year by year. Under natural conditions in the field, especially under unfavorable conditions, keeping seedlings uniform and strong directly determines the production potential and benefits of rice. [0003] Due to the unpredictability of seed production, especially hybrid rice seeds, short-term storage of seeds is often required. Due to the phenomenon of aging an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 刘军高家东付华张友胜张艺馨
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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