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173 results about "Exoenzyme" patented technology

An exoenzyme, or extracellular enzyme, is an enzyme that is secreted by a cell and functions outside of that cell. Exoenzymes are produced by both prokaryotic and eukaryotic cells and have been shown to be a crucial component of many biological processes. Most often these enzymes are involved in the breakdown of larger macromolecules. The breakdown of these larger macromolecules is critical for allowing their constituents to pass through the cell membrane and enter into the cell. For humans and other complex organisms, this process is best characterized by the digestive system which breaks down solid food via exoenzymes. The small molecules, generated by the exoenzyme activity, enter into cells and are utilized for various cellular functions. Bacteria and fungi also produce exoenzymes to digest nutrients in their environment, and these organisms can be used to conduct laboratory assays to identify the presence and function of such exoenzymes. Some pathogenic species also use exoenzymes as virulence factors to assist in the spread of these disease causing microorganisms. In addition to the integral roles in biological systems, different classes of microbial exoenzymes have been used by humans since pre-historic times for such diverse purposes as food production, biofuels, textile production and in the paper industry. Another important role that microbial exoenzymes serve is in the natural ecology and bioremediation of terrestrial and marine environments.

Fungal fibrinolytic enzyme and cultivating method thereof

The invention discloses a fungal fibrinolytic enzyme and a preparation method thereof. The fungal fibrinolytic enzyme is prepared by taking a Cordyceps militaris strain as a strain and performing liquid fermentation culture, separation and purification. The relative molecular weight of the fibrinolytic enzyme is about 21,000. The Cordyceps militaris fibrinolytic enzyme has good thrombolytic performance, is free from obvious acute toxicity, has prospects for clinical trial, development and application, and adds a new member to a rare fibrinolytic enzyme family. Particularly, a preferable fibrinolytic-enzyme Cordyceps militaris strain C.LSG-1 obtained through severe screening is rough in growth conditions, short in enzyme production cycle and capable of harvesting a large amount of Cordyceps militaris mycelium during enzyme production, and is the strain excellent in production performance. The Cordyceps militaris fibrinolytic enzyme is an extracellular enzyme which is particularly beneficial to subsequent separation and purification during preparation. The fungal fibrinolytic enzyme takes corn protein with extensive sources as a main culture medium, thereby having low cost for raw materials and providing a novel way for developing and utilizing the prior resources.
Owner:QIQIHAR UNIVERSITY

Preparation method for k-carrageenan oligosaccharide with low polymerization degree

The invention discloses a preparation method for k-carrageenan oligosaccharide with a low-polymerization degree. The preparation method comprises the following steps of: carrying out enzymolysis preparation on the k-carrageenan; and separating and purifying the degradation products to obtain pure k-carrageenan oligosaccharide mixing component. The preparation method is characterized in that carrageenan degradative enzyme is obtained from a marine microorganism Cellulophaga lytica strain: N5-2(NCBI (National Center of Biotechnology Information), GENBANKD registry number being GU129978) and used for degrading the k-carrageenan to obtain the k-carrageenan oligosaccharide mixture with the low polymerization degree. The enzyme is an extracellular enzyme, can be acted to beta-1,4 glucosidic bond of the k-carrageenan, wherein the acting sites are respectively octasaccharide and hexaose; and the product is low-polymerization degree oligosaccharide consisting of disaccharide repeating units. The preparation method disclosed by the invention is low in cost and simple. Compared with the methods such as chemical method and a physical method, the obtained product is single, the side products are less and the large-scale production is convenient.
Owner:WEIHAI KANGBOER BIOLOGICAL PHARMA

Method for increasing lipase expression through glycosylation modification as well as mutant enzyme and application thereof

The invention discloses a method for increasing lipase expression through glycosylation modification as well as a mutant enzyme and an application thereof and belongs to the field of enzyme engineering. The N-glycosylation mutation is performed on a leading peptide sequence of rhizopus oryzae lipase, the SAS and / or NT amino acid are / is respectively modified to an N-glycosylation site NGT and / or NLT, the extracellular protein concentrations of the obtained mutant enzyme proROLA, proROLB and proROLAB are increased by 211%, 188% and 233% compared with those of the un-glycosylated proROL, the enzyme activities when culturing in a fermentation tank are respectively 8210 U.mL<-1>, 8457 U.ml<-1> and 9366 U.mL<-1>, and the un-mutated proROL extracellular enzyme activity is almost zero. The rhizopus oryzae lipase provided by the invention has obviously increased lipase enzyme activity and can be used in the fields such as food, chemical engineering and biological energy source.
Owner:TAIXING YIMING BIOLOGICAL PRODS

Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation

InactiveCN1884509AIncrease fermentation gas productionPromote degradationHydrolasesMicroorganismsBiotechnologyMethane yield
The method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation belongs to preparation of supplementary exoenzyme used for marsh gas fermentation. The invention is to provide a method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation, which has the production bacteria with the following enzymatic vitality index: selecting wheat bran and rice bran as mixed culture medium, obtaining mixed bran seeds by pedigree breed two-stage enlargement culture of the bacteria with solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic vitality index, adding commodity enzyme preparation to reach enzymatic vitality index, and airing until the humidity is lower than 5%. The enzymatic vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug tyrosine / g / min. The bacteria can be obtained by bacteria and fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the methane tank fermentation raw materials, the rate of dissolution and degradation of the marsh gas fermentation raw materials can be improve, and the gas-producing rate of marsh gas fermentation, TS and VS gas-production potential and methane content in marsh gas can be increased.
Owner:YUNNAN NORMAL UNIV

PelB signal peptide mutant capable of improving protein secretion efficiency and application of pelB signal peptide mutant

The invention discloses a pelB signal peptide mutant capable of improving the protein secretion efficiency and an application of the pelB signal peptide mutant and belongs to the technical field of gene engineering. A sequence of a pelB mutant secretive expression signal peptide is as shown in SEQ ID NO.1. The signal peptide is capable of improving the extracellular enzyme activity of objective protein cyclodextrin glycosyltransferase by 1.6 times. The extracellular protein production capability of recombinant escherichia coli transformed by using the signal peptide is strengthened, and industrialized production is facilitated.
Owner:JIANGNAN UNIV

Calcium alginate-sodium carboxymethyl cellulose co-immobilized Phanerochaete chrysosporium strain and biological microcapsule of exoenzyme thereof, and preparation method of strain

The invention relates to a calcium alginate-sodium carboxymethyl cellulose co-immobilized Phanerochaete chrysosporium strain and a biological microcapsule of exoenzyme thereof, and a preparation method of the strain. The biological microcapsule comprises a shell and a shell inside core, wherein the shell material is a calcium alginate gel, and a Phanerochaete chrysosporium exoenzyme crosslinking aggregate is embedded inside the calcium alginate gel; and the shell inside core is a calcium chloride-sodium carboxymethyl cellulose water solution, and Phanerochaete chrysosporium cells and an extracellular crosslinking enzyme aggregate are dispersed inside the calcium chloride-sodium carboxymethyl cellulose water solution. In the biological microcapsule preparation process, the Phanerochaete chrysosporium exoenzyme is directly prepared into the crosslinking enzyme aggregate, thereby omitting the step of purification of the lignin degrading enzyme, effectively lowering the production cost and enhancing the degradation efficiency since the crosslinking enzyme aggregate and Phanerochaete chrysosporium form a close relation.
Owner:山东济清科技服务有限公司

Genetically engineered bacterium for producing linear malt oligosaccharide generating enzyme and application of genetically engineered bacterium

ActiveCN105950528AMethod securityHigh starch conversion rate and product purityBacteriaMicroorganism based processesOligosaccharideBiotechnology
The invention discloses a genetically engineered bacterium for producing a linear malt oligosaccharide generating enzyme and application of the genetically engineered bacterium, and belongs to the technical field of microbial engineering. According to the genetically engineered bacterium, secretory expression of the linear malt oligosaccharide generating enzyme in bacillus subtilis is achieved, a method is safe and efficient, the hydrolytic activity reaches 36.6 U / mL, and the enzyme producing capacity is 10 times or above of that of an original bacterium; the obtained recombinant enzyme is an extracellular enzyme, convenience is brought for extraction and purification of large-scale production in the later period, and the product meets the food requirement. Starch is hydrolyzed by the purified recombinant linear malt oligosaccharide generating enzyme to generate linear malt oligosaccharide syrup which does not contain glucose and contains a high proportion of maltopentaose, and the linear malt oligosaccharide syrup can be directly used for producing functional food and also can be used for producing products such as high-purity linear malt oligosaccharides.
Owner:JIANGNAN UNIV

Method for efficiently synthesizing L-theanine through recombined corynebacterium crenatum

The invention discloses a method for efficiently synthesizing L-theanine through recombined corynebacterium crenatum, and belongs to the field of genetic engineering and enzyme engineering. According to the method, it is firstly verified that GGT signal peptide of a bacillus subtilis source can achieve secretory expression in a C.glutamicum system. Meanwhile, extracellular enzyme activity of recombinant bacteria C.glutamicum SDNN403/pXMJ19-tacM-ggt is about two times that of C.glutamicum SDNN403/pXMJ19-ggt, and it indicates that a tac Mpromoter is more beneficial for synthesis of GGT enzyme activity. Substrate flow and strategic high-yield L-theanine are adopted, a conversion system contains GGT with the concentration of 0.8 u/mL, the pH is 10, the temperature is 37 DEG C, and 22 mmol/L L-theanine and 66 mmol/L ethylamine are supplemented every other two hours from 0 h. The maximum theanine yield of 118 mmol is achieved when batch flow is added for 12 h, and the conversion rate is 92.8%. The secretory expression of gamma-glutamyltranspeptidase genes (ggt) of the B.subtilis source in C.glutamicum SDNN403 is achieved for the first time, and the highest yield of L-theanine reported currently and synthesized through recombined C.glutamicum is obtained by adding the substrate in batch flow.
Owner:JIANGNAN UNIV

Method for increasing content of propionic acid in batch fermentation acid-producing liquid

ActiveCN102776247APropionic acid content enhancedFacilitate the hydrolysis of sugarMicroorganism based processesFermentationMicroorganismExtracellular
The invention belongs to the technical field of environmental protection and relates to a method for increasing content of propionic acid in batch fermentation acid-producing liquid. The method includes the steps of firstly, mixing kitchen waste and thickened sludge, adding mixture into an anaerobic hydrolysis fermenter for anaerobic fermentation, centrifugally separating mixture, and taking supernate for standby; secondly, activating and enriching propionibacterium seed broth; thirdly, sterilizing the supernate obtained in the step 1, inoculating the propionibacterium seed broth obtained in the step 2 to the supernate, and fermenting in an anaerobic fermenter to generate propionic acid; and fourthly, centrifuging the fermented mixture to obtain the fermented supernate rich in propionic acid after fermentation. The sludge and the kitchen waste are subjected to primary fermentation under the weak alkaline condition, and microorganism extracellular enzyme hydrolysis of macromolecular organic matters such as microorganism exoenzyme hydrolysate, protein and fat is facilitated. The content of the propionic acid in the fermented supernate is 3.56-9.95g COD per L of total acid in the supernate.
Owner:TONGJI UNIV

Method for obtaining enzyme mutant with high expression, high activity and high stability

The invention discloses a method for obtaining an enzyme mutant with high expression, a high activity and high stability, and belongs to the technical field of genetic engineering. By means of a functional polypeptide library which is constructed on the basis of SAPs, the whole process of obtaining the enzyme mutant is quick, efficient and convenient; meanwhile, compared with wild-type alkaline pectinase, the activity of extracellular amylase of the fused enzyme mutant of alkaline pectinase is maximally improved by 15.32 times, the stability is maximally improved by 3.86 times, and the specific enzyme activity is improved by 2.55 times; the activity of intracellular amylase of the fused enzyme mutant of lipoxygenase is maximally improved by 2.49 times, the stability is maximally improved by 3.13 times, and the specific enzyme activity is improved by 0.9 time; the activity of extracellular amylase of the fused enzyme mutant of asparaginase is maximally improved by 2.25 times, the stability is maximally improved by 3.56 times, and the specific enzyme activity is maximally improved by 1.34 times.
Owner:JIANGNAN UNIV
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