56 results about "Shiraia bambusicola" patented technology

The present invention relates to a single-cell protein biological feed and a preparation method thereof. Particularly, the single-cell protein biological feed is prepared from rhizoma atractylodis medicinal residues and prepared by a two-step fermentation method using shiraia bambusicola, and trichoderma asperellum and candida utilis. The feed has effects of improving an egg laying rate of layingchickens, enhancing immunity, etc.

The invention relates to the field of bio-fermentation, in particular to a method of producing hypocrellin pigments and polysaccharides simultaneously with solution fermentation. In the art of producing hypocrellin pigments and polysaccharides simultaneously with solution fermentation of the invention, bottle-shaking culture is firstly proceeded, convolution culture is the second step, the enlarging culture of the shaking bottle is the third step and fermentation culture is the final step. In the step of bottle-shaking culture, strain of the hypocrellin is utilized as parent strain, bacteria on the inclined surface of the tube are introduced to a shaking bottle inside which liquid medium is arranged, and the charging coefficient of the shaking bottle is 0.1 to 0.25. In the step of convolution culture, the rotation speed of the convolution culture is 150 to 250 turnings every minute and the culture temperature is 22 to 27 DEG C. In the third step of enlarging culture of the shaking bottle, the culture material achieved by the bottle-shaking culture is shifted to the culture medium of the enlarging culture of the shaking bottle to proceed culture, the inoculation amount is 2-10%, the rotation speed of a rotation bed is 150-250 turnings every minute, the culture temperature is 22 to 27 DEG C, and the culture period is 72 to 120 hours. In the final step of fermentation culture, the culture material achieved by the enlarging culture of the shaking bottle is transferred to a fermentation pot inside which fermentation culture medium is installed to be cultured, the inoculation amount is 5-10%, and the culture temperature is 22 to 27 DEG C. The hypocrellin fermentation liquid is achieved after the fermentation is finished, and the hypocrellin pigments and polysaccharides are respectively achieved by separating and purifying the achieved fermentation liquid.

The invention discloses a method for preparing a perylenequinone pigment under the catalysis of Shiraia bambusicola and belongs to the technical field of bioengineering. In the method, naphthoquinone, cinnamic acid and the like which form a substrate are used to prepare the perylenequinone pigment under the catalysis of the Shiraia bambusicola. The catalytic process is implemented by: A), adding the substrate into culture system in a cell liquid culture process, wherein the perylenequinone pigment yield is up to 2.9g / L and the transformation rate is up to 9.7 percent; or B), placing cells obtained by liquid fermentation culture in a reaction system having the substrate, wherein the perylenequinone pigment yield is up to 35.3g / L and the transformation rate is up to 39.7 percent; or C), adding the substrate into a culture system in a cell solid culture process, wherein the perylenequinone pigment yield is up to 1.5g / 100g and the transformation rate is up to 46 percent. The method adopts a perylenequinone pigment-producing Shiraia bambusicola strain and specific substrate transformation to produce hypocrellin, is high in transformation rate, low in cost, less in step and applicable to industrial production and has excellent industrial application prospect.

The invention relates to a method for manufacturing a bamboo product, namely bamboo sticks, and belongs to a bamboo stick processing method. Cut surface green bamboo chips and shiraia bambusicola chips are subjected to fiber forming, water milling, bleaching and drying to obtain the bamboo sticks. The water milling treatment can effectively remove burrs and stains on the surfaces of the bamboo sticks, so that the produced bamboo sticks have smooth surfaces without the burrs, and high product quality, save the steps of scraping surface green and stewing, simplify process flow, and save raw materials and processing cost; and the waste materials obtained after the water milling can be directly used for paper making, so that no pollution is caused. The processed bamboo sticks can be widely used in bamboo products such as bamboo mats, bamboo curtains, bamboo carpets, bamboo-splint woven handicraft articles and so on.

The invention relates to a bamboo splint processing method, and belongs to the bamboo splint processing method. Cut surface green bamboo chips and shiraia bambusicola chips are subjected to fiber forming, bleaching, water milling and drying to obtain bamboo splints. The water milling treatment can effectively remove burrs and stains on the surfaces of the bamboo splints, so that the produced bamboo splints have smooth surfaces without the burrs, save the steps of scraping surface green and stewing, simplify process flow, improve processing and utilization rate of bamboo raw materials and save resources; and the waste materials obtained after the water milling can be directly used for paper making, so that no pollution is caused and environmental protection is ensured. The processed bamboo splints can be widely used in bamboo products such as bamboo mats, bamboo curtains, bamboo carpets, bamboo-splint woven handicraft articles and so on.

The invention discloses a technology for improving hypocrellin yield in shiraia bambusicola by using a polystyrene type nonpolar adsorption resin, wherein the technology comprises: carrying out activating culture for a strain, carrying out liquid seed culture, and carrying out co-culture fermentation for the strain and the adsorption resin. The method is characterized in that: with the co-culture fermentation, the resin can absorb the hypocrellin secreted by the shiraia bambusicola, and the hypocrellin content in the shiraia bambusicola can be improved so as to improve the hypocrellin yield. Therefore, with the present invention, the synthesis of the hypocrellin in the shiraia bambusicola can be effectively stimulated, the hypocrellin production in the culture system can be improved, the yield is high, the cost is low, the steps are simple, and the method can be used for the industrial production, and has a good industrial application prospect.

The invention discloses a plant protective agent prepared from the following raw material medicines in parts by weight: 20-40 parts of radix rehmanniae recen, 2-6 parts of herba lycopi, 6-17 parts of cortex moutan, 2-13 parts of radix sophorae flavescentis, 6-17 parts of sowthistle tasselflower herb, 2-8 parts of herba centipedae, 2-8 parts of fructus xanthii, 1-2 parts of plantain herb, 2-6 parts of shiraia bambusicola henn, and 1-2 parts of raw oysters. In addition, the invention also discloses a preparation method of the plant protective agent. The plant protective agent is prepared by adopting the pure traditional Chinese medicines, is green, has no pollution, and is rich in raw material sources, simple in preparation process, relatively low in cost, and suitable for agriculture industrialization production. The plant protective agent has relatively strong inhibitory activity against botrytis cinerea pers and pseudoperonospron cubensis, is a non-toxic grade, has a dosage form of a liquid agent, is safe to use, has no pollution to the environment and vegetables, and is the plant protective agent having a promotional value.

A method for reducing the toxicity of strychnos nux-vomica seeds by solid fermentation with medicinal fungi comprises the following steps of: activating and culturing schizophyllum commune, shiraia bambusicola, coriolus unicolor, cantharellus cibarius, beauveria bassiana and cordyceps cicadae for fermentation; oven-drying strychnos nux-vomica seeds, pulverizing, soaking in water, and autoclaving; respectively inoculating the above fungi on the treated strychnos nux-vomica seeds, performing solid fermentation under certain conditions, collecting respective fermentation product, and drying at low temperature to obtain medicinal fungal substances. The inventive method is simple and reasonable, and can obtain six medicinal fungal substances with reduced toxicity of strychnos nux-vomica seeds suitable for treatment purpose. The medicinal fungal substances have toxicity remarkably lower than that of strychnos nux-vomica seeds, have definite analgesic and antiphlogistic effects, and can keep the original main drug effect of Strychnos nux-vomica seed.

The invention discloses a bamboo parasitic fungus and application thereof in fermentation production of hypocrellin. Three bamboo parasitic fungi are separated from Shiraia bambusicola respectively collected from Huangshan region of Anhui province, Quzhou region of Zhejiang province and Yinbin region of Sichuan province, are cultured by virtue of a basal culture medium and is processed by virtue of ion beams, and therefore, a strain with the highest vigorous activity is obtained, is named as Shiraia bambusicola NJTECH-1 and has been preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC NO. 11617. The bamboo parasitic fungus has very high metabolic activity and is applied to the fermentation production of the hypocrellin, and the yield of the hypocrellin is high. The field waste straws and animal dung can be taken as fermentation substrates, so that the quantity of incinerated straws can be reduced, and the environmental pollution can be reduced while the cost is saved.

The invention belongs to the technical field of microorganisms, and discloses a strain of Shiraia bambusicola MH-02 with high yield of Hypocrellin A. The classified nomenclature of the strain is Shiraia bambusicola, and the preservation number is CDMCC No.60438. The strain is used for converting glycerinum into the high value-added Hypocrellin A, the conversion rate is high, and an environment-friendly and efficient pathway is provided for comprehensive utilization of the glycerinum.

The invention discloses a deer blood wine for treating rheumatism and gout and a preparation method thereof, wherein the deer blood wine comprises the following raw materials in parts by weight: 20-30 parts of deer blood, 10-15 parts of aspongopus, 15-20 parts of scorpion, 8-12 parts of ant, 15-25 parts of ptyas dhumnades, 10-15 parts of pangolin, 5-10 parts of cibotium rhizome, 15-20 parts of jujube peel, 8-12 parts of muscardine silkworm, 20-30 parts of eucommia, 15-25 parts of taxillus, 12-16 parts of achyranthes bidentata, 9-14 parts of fibraurea recisa, 18-24 parts of vegetable sponge of luffa, 10-15 parts of seatung bark, 20-25 parts of phellodendron, 15-20 parts of rhizoma atractylodis, 16-22 parts of fructus liquidambaris, 10-15 parts of Chinese parsnip root, 8-12 parts of peony vine, 20-30 parts of campsis grandiflora loisel, 15-25 parts of fish poison yam rhizome, 14-18 parts of shiraia bambusicola, 10-15 parts of botrychiam ternatum and 400-500 parts of wine of 50 DEG to 60 DEG. The deer blood wine in the invention has effects of clearing heat and removing toxicity, promoting flow of qi and blood circulation, inducing menstruation to relieve menalgia and dispelling wind and eliminating dampness. The deer blood wine has a good curative effect in the treatment of gout, rheumatoid arthritis, rheumatoid arthritis, numbness of limbs, traumatic injury, lumbar and leg ache and other symptoms.

The invention relates the method for making anthraquinone pigments with bamboo yellow bacterium submerged cultuse, submerged cultuse liquid and anthraquinone pigments 1, 5- dihydroxy-3- methoxy-7- methyl-anthraquinonyl. The method comprises the following steps: using bamboo yellow bacterium as strain, carrying out fluid culture, fluid enlargement culture, one-level seed culture and submerged cultuse culture, separating the fermentation liquor, purifying, and getting the products. The invention first produces the anthraquinone pigments (I), which is brown solid. The products can be used as food additives, and have oxidation resistance and bacteriostasis.

A method for preparing hyperoxide dismutase (SOD) by fermenting shiraia bambusicola belongs to the technical field of bioengineering. The method adopts shiraia bambusicola strain for preparing the SODwhich is mainly exoenzyme under the condition of liquid state fermentation. During the fermentation, the SOD yield can be improved by adding H2O2, surfactant or alkane or by illumination. The maximumSOD yield can be 800 U / mL. During the fermentation, the strain can simultaneously generate quinochromes which can have a yield as high as 1.1 g / L, wherein hypocrellin A accounts for 90 percent and hypocrellin B accounts for 5 percent. Tabasheer is an officinal fungus and mainly generates hypocrellins and other quinochromes by means of fermentation, thereby having high officinal value and healthcare function. The fermentation of the tabasheer to prepare the SOD has the advantage of short fermentation period, the activity of the SOD in fermentation liquid is relatively high, and the quinochromes can be simultaneously generated. The method has a good prospect for industrial application.

The invention discloses a bis(p-benzoquinone) compound and an extraction method thereof. The bis(p-benzoquinone) compound is named as 2,7-dihydroxy-3,6,9-trimethyl-9H-xanthene-1,4,5,8-tetraone. The bis(p-benzoquinone) compound with the molecular weight being 316 and unsaturation degree being 11 can form orange red needle crystals in an ethyl acetate solution, is represented as a molecular formula of C16H12O7 and is represented as a structural formula containing one oxygen heterocycle, two hydroxys, three methyls and four ketone groups. The extraction method of the bis(p-benzoquinone) compound includes isolating a haematochrome-producing hypha fermentation solid from a fruiting body of Shiraia bambusicola Henn., extracting through ethyl acetate to obtain extract, adding the extract into petroleum ether for sufficient dissolving, centrifuging to remove a supernatant, subjecting a precipitate to ethyl acetate dissolving and then column chromatography on silica gel, concentrating to crystallize and using reversed-phase high performance liquid chromatography for detecting purity so as to obtain the novel high-purity compound. The bis(p-benzoquinone) compound has a high free radial scavenging capability and provides a lead compound resource for developing new drugs as a natural product of a novel structure.

The invention provides a traditional Chinese medicinal preparation for treating dysthymia. The traditional Chinese medicinal preparation is prepared from the following traditional Chinese medicinal materials: fossil fragments, rose flowers, shiraia bambusicola, rhizoma nardostachyos, concha haliotidis, herba epimedii, peach kernels, rhizoma polygonati, radix salviae miltiorrhizae, antelope's horn, pinellia ternata, radix bupleuri, Chinese yam, saffron crocus, albizia flowers, polygala tenuifolia, lotus seeds, the fruits of Chinese wolfberry, rhizoma gastrodiae, the root of bidentate achyranthes, prepared rehmannia root, rhizoma anemarrhenae, cinnabar, oyster, mint and spina date seeds. The traditional Chinese medicinal preparation has good clinical effects for patients suffering from depressive disorder at various stages, and has good treatment effects at the medication initial stage, the symptoms including insomnia, early awakening, dreaming and agriothymia are alleviated, the pain of the patient is greatly reduced, and the traditional Chinese medicinal preparation has the outstanding effect of being free of toxic and side effects, can rapidly improve the disease symptoms of the patients, has the advantages of significant curative effects and small possibility of relapse, and is low in treatment cost.

The invention relates to a method for producing laccase by fermenting Shiraia bambusicola, belonging to the technical field of bioengineering. The invention uses Shiraia bambusicola strain Shiraiasp.SUPER-H168 to obtain the laccase by liquid state fermentation, wherein the preservation number of the Shiraia bambusicola strain is CCTCCNO:M207104 which is published in ZL200710132510.4. The study on the laccase produced by Shiraia bambusicola is not reported. The Shiraia bambusicola is a medical fungus, which has high medicinal value and health efficacy. The method for producing the laccase by fermenting the Shiraia bambusicola has the advantages of short fermentation period and high enzyme activity, meanwhile, SOD with high activity and perylenequinonoid pigment can be produced in the fermentation process, and the development prospects are broad.

The invention provides a composition for cleansing face, tendering skin and improving skin metabolism. The composition is composed of the following raw materials, by weight, 5-15 parts of water, 1-3 parts of a lotus extract, 3-5 parts of rose oil, 2-4 parts of a calendula extract, 1-3 parts of a Shiraia bambusicola stomata extract, 1-3 parts of a Thalictrum aquilegifolium extract, 2-4 parts of tea tree essential oil, 2-5 parts of a usnea extract, 3-5 parts of a wheat germ extract, 3-5 parts of glycerin, 2-4 parts of glycoprotein and 1-3 parts of citric acid. The composition can wash off cosmetic and impurities on face to bring a fresh and comfortable feeling to the skin. In addition, the composition has characteristics of improving skin microcirculation and the like. The composition can safely and effectively promote skin metabolism, brighten skin tone and eliminate color spots.

A medicament for treatment of renal failure and a preparation method therefor. Shiraia bambusicola, Fossilia ossis Mastodi, Angelica pubescens, dried tangerine peel, Hedyotis diffusa, Gynostemma pentaphyllum, and Bauhinia championii seed are used as active ingredients, while Lophatherum gracile, Glycyrrhiza uralensis, smoked Prunus mume seed, Pseudostellaria heterophylla, Pueraria lobata Ohwi、lotus leaf, Citrus medica var. sarcodactylis, Leonurus Artemisia, Andrographis paniculata, Gardenia jasminoides, Bambusae Caulis in Taeniam, papaya, and processed Rehmannia glutinosa are used as excipients. With the traditional Chinese medicine principles that kidneys are the congenital foundation and that kidneys rule water, kidney vital energy is cultivated and kidney fluids are regulated for differentiation and treatment of renal failure. Side effects are mitigated, great efficacy is provided, and patient suffering is alleviated.

The invention provides a drug for treating rheumatism and rheumatoid and a preparation method thereof. The drug is prepared according to the following steps of: combining 14 Chinese herbal medicines including cooked radix aconiti carmichaeli, herba asari, ramulus cinnamomi, radix aconite lateralis preparata, rhizoma zingiberis, shiraia bambusicola P.Henn, radix achyranthis bidentatae, siegesbeckia orientalis, ramulus mori, common threewingnut root, scarabaeoidea larva, sulfur, pangolin scales and dried and cooked strychnospierriana with three western medicines including vitamin B1 tablets, metoclopramide tablets and diclofenac sodium tables; and grinding or decocting with water and extracting to form paste, and adding conventional auxiliary materials to prepare different preparations. The drug combines traditional herbal medicines and western medicines, is scientific and particular in formula as well as simple and reasonable in preparation method and has the advantages of both traditional herbal medicines and western medicines; side effects are relatively less, and the curative effects supplement and complement each another; and the drug has a quick effect and a lasting medicinal effect and can effectively treat and relive rheumatism and rheumatoid.

The invention discloses a formula and a preparation process of a medium for culturing medical shiraia bambusicola henn and belongs to the technical field of processing of mediums. The formula is characterized in that the medium contains 21 to 37 percent of nutrient solution, 8 to 19 percent of sugar, 2 to 11 percent of fungus, 5 to 16 percent of synergistic component and 30 to 56 percent of mineral water. According to the formula and the preparation process of the medium for culturing the medical shiraia bambusicola henn, the medium contains various nutritional components which are required by the survival of shiraia bambusicola henn, the nutrients required by growth of shiraia bambusicola henn can be provided, the culture period can be shortened, the yield and the quality of hypocrellin can be improved, the medium is good in culture effect, the quality of the cultured hypocrellin does not have obvious difference with that of the shiraia bambusicola henn hypocrellin in nature, and the medium is easy and convenient to prepare, safe and effective, convenient to use, and low in production cost. The formula of the medium can be applied to artificial culturing of shiraia bambusicola henn hypocrellin in a laboratory and industrial large-area production and development of shiraia bambusicola henn hypocrellin.

The invention discloses an accompanying bacterium for increasing fermentation yield of hypocrellin and application thereof; the accompanying bacterium has classification name of Pseudomonas fulva, which is collected under CGMCC No. 13931. Viable ones of the accompanying bacteria having different concentrations are added into Shiraia bambusicola fermentation liquid cultured for 5-7 days, and culturing is continued to obtain hypocrellin. The yield of the hypocrellin can be increased effectively, and the accompanying bacterium is nontoxic and harmless to human and livestock and is free of environmental pollution.

The invention belongs to the technical field of microbial fermentation, and particularly relates to Shiraia bambusicola with high yield of hypocrellin, and a cultivation method and application of theShiraia bambusicola. The Shiraia bambusicola with high yield of hypocrellin is preserved in the China General Microbiological Culture Collection Center with the classification name of Shiraia bambusicola, and the preservation number is CGMCC No.18808. The Shiraia bambusicola can be fermented to obtain high-yield hypocrellin, the total amount of hypocrellin can reach 4762.9mg / 100g, and the obtainedhypocrellin includes hypocrellin A, hypocrellin B, hypocrellin C and hypocrellin D at the same time.

The invention discloses a stomach nourishing traditional Chinese medicine composition. The stomach nourishing traditional Chinese medicine composition comprises 2-3 parts of eleocharis dulcis, 1-3 parts of white honey, 1-2 parts of rhizoma corydalis, 1-2 parts of shiraia bambusicola henn, 3-5 parts of herba pogostemonis, 1-3 parts of trapa incisa, 2-4 parts of capsella bursa-pastoris roots, 3-5 parts of physalis alkekengi, 1-2 parts of peanut coats, 1-2 parts of santonin, 3-5 parts of royal jelly freeze-dried powder, 1-2 parts of herba gendarussae, 1-3 parts of folium pyrrosiae, 2-3 parts of rumex patientia roots, 3-5 parts of poria and 1-2 parts of liquidambar formosana hance resin. Owing to the mode, the stomach nourishing traditional Chinese medicine composition has the advantages that excellent stomach nourishing effects can be realized by the stomach nourishing traditional Chinese medicine composition, the stomach nourishing traditional Chinese medicine composition is free of side effects, and appetite can be improved.

The invention discloses a donkey-hide gelatin spring roll capable of activating blood circulation. The spring roll comprises the following raw materials in parts by weight: flour 30-40, nutritional additives 4-8, donkey-hide gelatin 1-2, black peanut 4-6, sugarcane leaves 2-3, rice milk 3-4, whole hulless oat 1-2, crab roe 3-4, sweet almond 2-3, soya-bean milk 3-4, black locust honey 1-2, pea shoots 2-3, Shiraia bambusicola 0.7-2.5, Codonopsis pilosula root 1-2.1, Ficus pumila 0.2-1.4, kelp 0.5-1.4, sweet broomwort 1-2.3, and a proper amount of peanut oil and refined salt. The raw materials such as donkey-hide gelatin, black peanuts and soya-bean milk are added into the filling of the spring roll, so as to increase the nutritional value of the spring roll. The spring roll also contains various health care components such as Shiraia bambusicola, Codonopsis pilosula root, Ficus pumila, kelp and sweet broomwort, so as to endow the spring roll with good functions of clearing heat and removing toxicity, diminishing swelling and preventing perspiration, promoting blood circulation and dredging collaterals.