279 results about "Candida candida" patented technology

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu / g; lactobacillus acidophilus is not less than 1.0*10cfu / g; lactobacillus plantarum is not less than 1.0-10cfu / g; bacillus natto is not less than 1.0*10cfu / g; aspergillus niger is not less than 1.0*10cfu / g; and candida utili is not less than 1.0*10cfu / g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention discloses a microbial deodorizing inoculant which is used in refuse sites, public toilets and livestock / poultry cultivation farms. The microbial deodorizing inoculant contains Candida utilis of which the collection number is ACCC 20060, Lactobacillus plantarum of which the collection number is ACCC 11016, lactic streptococci of which the collection number is ACCC 10653, cerevisiae fermentum of which the collection number is ACCC 21135, Streptococcus thermophilus of which the collection number is ACCC 10651, Bacillus licheniformis of which the collection number is ACCC 01870, and Bacillus natto of which the collection number is ACCC 19833. The microbial deodorizing inoculant absorbs and degrades generated malodorous gas hydrogen sulfide and ammonia gas, eliminates the malodor of the garbage and garbage leachate, inhibits the putrefaction bacteria from growth, basically prevents the malodorous gas from generation, and forms a disease-spread-free refuse piling environment capable of eliminating the malodor.

The invention discloses a preparation method for a sludge aerobic composting composite inoculums. According to the method, the Bacillus subtilis (No. 10066), the Bacillus licheniformis (No. 10103), the Lactobacillus acidophilus (No. 6075), the Candida utilis (No. 31272), and the Phanerochaete chrysosporium (No. 40719) that are preserved in China Center of Industrial Culture Collection (CICC) are subjected to individual culture, and are then mixed in proportion and inoculated into a fermentation culture to perform expanded culture, thus obtaining the composite inoculum. At the initial stage of inoculating the composite inoculums to sludge aerobic composting, the time for the compost body to reach a high temperature period is advanced by 4-6 days, the compositing period is shortened by 11-14 days, the stack moisture content is decreased by 18%-25%, and the total nitrogen content of the compost body is increased by 15%-20%, thus improving the composting effect.

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention relates to a complex microorganism additive as well as a preparation method and application thereof, belonging to the biological material technical field of application of a microecological modulator in cultivating domestic animals. The preparation method thereof comprises the following steps: taking 30-60% of Saccharomyces cervisiae, 5-30% of Candida utilis, 5-30% of Rhodopseudom onas Palustris, 5-30% of Lactobacillus plantarum, 5-40% of Leatobacillus acidophilus and 5-25% of Bacillus subtilis; and sequentially carrying out primary bacteria strain culture, secondary fermentation and tertiary fermentation on the bacteria strains so that the bacterial number in fermentation liquor reaches 8-10*108 / mL, thus obtaining the complex microorganism additive 808. Long-term on-site experiments prove that the product has reasonable formula, stable efficacy, disease prevention and resistance, improved quality and capability of promoting growth of animals and improving yield of animal products, survival rate and feed conversion rate.

The invention provides a plant bacteriostatic composition and application thereof in cosmetics. The plant bacteriostatic composition is obtained by compounding a licorice extract, a honeysuckle extract and a Mangnolia officinalis extract. The plant bacteriostatic composition has good bacteriostatic effect and particularly has a synergistic effect of inhibiting escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger. The plant bacteriostatic composition can be applied to skin-care and washing cosmetics, reduces the adding amount of original preservatives and enables a product to have both antibacterial and anti-inflammatory effects.

The invention relates to a multi-strain microorganism pig feed additive and a production method thereof. According to the additive, bacillus subtilis, lactobacillus delbrueckii, saccharomyces cerevisiae, candida and photosynthetic bacteria are inoculated into a fermentation culture medium according to a ratio of 2:1:1:1:2, and are prepared into a finished product through solid fermentation. The microorganism preparation is reasonably matched, various microorganisms in the preparation can bring respective effects into play and depend on one another, the overall advantage of the microorganism preparation can be brought into play, the growth development and the improvement of the anti-disease capability of a host are facilitated, and the feed additive is safe to use and can improve the immunity of animals, prevent and resist diseases, prompt animal growth, improve the feed utilization rate and improve the feed quality.

The invention discloses a crop straw decomposition agent. The crop straw decomposition agent is composed of the following components by mass percent: 20%-30% of bacillus subtilis, 20%-30% of aspergillus niger, 10%-15% of candida utilis, 15%-20% of trichoderma viride, 10%-15% of aspergillus oryzae and 1%-2% of cellulase. In order to solve the problems such that current straws are directly returned to fields, and the temperature rise is slow, the decomposition is slow and the decomposition effect is poor when the straws are used for composting an organic fertilizer, crop straw decomposition is tested for a plurality of years, microorganism strains and biological enzyme preparations are continuously screened, and finally, the bacillus subtilis, the aspergillus niger, the candida utilis, the trichoderma viride, the aspergillus oryzae and the cellulase are prepared into the crop straw decomposition agent according to the scheme. The four fungi are not antagonistic and combined with the cellulase to have a synergistic interaction effect and the straws can be rapidly decomposed.

The invention discloses a strain of yeast with strong capacities of tolerating, enriching and converting organic selenium, which is named Candida utilis CUM and has been preserved in China center for type culturecollection on April 19, 2010 with the preservation number of CCTCC M 2010090. The Candida utilis CUM has the bacterial characteristics that: cells are round, oval or sausage-shaped, and performs vegetative propagation in a mode of multi-edge germination to form pseudohypha; wort solid culture is carried out, and the bacterial colony is milk white, has smooth and moist surface, gloss or no gloss, and neat or hypha-shaped edges; the Candida utilis CUM is chemoheterotrophic, and can ferment glucose, cane sugar and raffinose; and the Candida utilis CUM is facultatively anaerobic, performs aerobic respiration under the aerobic conditions and performs alcoholic fermentation under the anaerobic conditions. The Candida utilis has strong selenium enriching capacity and good stability, can be used for producing selenious yeast, and is a strain with high research and development value.

The invention discloses composite bacteria for removing oil from kitchen waste and a preparation method for the composite bacteria. The preparation method comprises the following steps of: performing high-density culture on strains of trichoderma viride, bacillus subtilis, black rhizopus, photosynthetic bacteria, candida lipolytica, bacillus licheniformis, nitrosomonas europaea and nitrobacter winogradskyi respectively to obtain microbial dry hypopus powder; and mixing 15 to 20 weight parts of trichoderma viride, 10 to 15 weight parts of bacillus subtilis, 25 to 40 weight parts of black rhizopus, 5 to 10 weight parts of photosynthetic bacteria, 5 to 10 weight parts of candida lipolytica, 10 to 15 weight parts of bacillus licheniformis, 5 to 15 weight parts of nitrosomonas europaea and 5 to 15 weight parts of nitrobacter winogradskyi to obtain the composite bacteria for removing the oil from the kitchen waste. The composite bacteria can quickly decompose lipid ingredients in the kitchen waste when used for treating the kitchen waste; and compared with the prior art, the method has the advantages that: the production investment and the production cost are low, and the method is convenient to use.

The invention discloses an environmentally-friendly composite microorganism preparation for eliminating house-fitment pollution. The composite microorganism preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus acidophilus, 15 to 35 percent of candida utilis, 5 to 25 percent of bacillus licheniformis, 10 to 25 percent of streptomyces albulus and 15 to 30 percent of rhodopseudomonas palustris and is obtained by compositely culturing the bacterial components by a fermentation process which combines anaerobic fermentation with aerobic fermentation.

The present invention relates to a single-cell protein biological feed and a preparation method thereof. Particularly, the single-cell protein biological feed is prepared from rhizoma atractylodis medicinal residues and prepared by a two-step fermentation method using shiraia bambusicola, and trichoderma asperellum and candida utilis. The feed has effects of improving an egg laying rate of layingchickens, enhancing immunity, etc.

The invention provides a microbial organic fertilizer and a preparation method thereof. The preparation method comprises the steps of strain culture, compounding and liquid submerged fermentation, and belongs to an agricultural integrated production technique. 15-20% of Bacillus mucilaginosus, 15-18% of Bacillus megaterium, 15-18% of Azotobacter chroococcum, 10-13% of Saccharomyces cerevisiae, 10-13% of Candida utilis, 15-18% of Lactobacillus plantarum and 10-13% of actinomycete are respectively subjected to primary and secondary strain culture and tertiary liquid submerged fermentation until the bacterial count in the fermentation liquid reaches 10-15*10<8> / mL, thereby obtaining the microbial organic fertilizer (707(Y)). By adopting the advanced liquid submerged fermentation technique and using the strains with high stability and functionality, which are screened from the natural world over the years and have the advantages of many varieties and convenient application method, the invention can promote the plant growth, improve the fruit quality, promote prematurity, increase the yield, enhance the utilization ratio of the fertilizer, improve the ecological environment of soil, and have certain effects of disease prevention and disease resistance.

The invention relates to an apple pomace microbial culture starter, its preparation method and a method for producing biological feed by the apple pomace microbial culture starter. The apple pomace microbial culture starter comprises 1-15% of candida, 1-15% of geotrichum candidum, 1-15% of Aspergillus niger, 1-15% of Aspergillus oryzae and 1-15% of bacillus subtilis. The method utilizes a pomace multi-bacterium synergistic biological fermentation technology. The feed culture starter contains acid-forming bacteria, cellulose decomposition bacteria, protein production bacteria and disease-resistant health care bacteria and through synergism of the bacteria, a pomace conversion utilization rate is improved, a digestion rate is improved, crude fiber content is reduced and crude protein content is improved. The biological feed prepared by the apple pomace microbial culture starter contains comprehensive nutrients, is green and safe, has a low cost and high benefits, substantially reduces hoof diseases of female animals bred in the feeding farm, improves a reproductive rate, is suitable for large scale and industical production and has a wide application prospect.

The invention relates to biological feed, in particular to a biological fermented feed and a preparation method thereof. The method consists of: subjecting Aspergillus niger, Bacillus licheniformis, Bacillus coagulans, Bacillus subtilis, Lactobacillus plantarum, and Candida utilis respectively to two-stage liquid fermentation culture, and then conducting three-stage solid fermentation culture to obtain fermented culture, i.e. biological feed. The fermented culture provided by the invention utilizes neural network, pattern recognition, genetic algorithm and other optimization methods to optimize the fermentation conditions, a biosynthesis network is constructed according to the principle of metabolic engineering, and pathway analysis and metabolic flux control are carried out on the synthetic network on the basis, and enzymolysis, anaerobism, aerobiosis, bacterium cultivation and other processes are employed, thus increasing the protein content, reducing the content of crude fiber, improving the palatability and fragrance, increasing probiotics, and removing toxins from feed raw materials.

The invention discloses a microbial preparation for saline-alkali soil improvement and an application method thereof. The microbial preparation for saline-alkali soil improvement is prepared from a composite microbial inoculant composed of Bacillus licheniformis, Candida utilis and Lactobacillus plantarum, sewage treatment plant sludge and straw stalk. The application method of the preparation is simple: the composite microbial inoculant, sludge and straw stalk are mixed and applied into the trench of the saline-alkali soil, so that the microbial preparation can perform metabolism and reproduction in the saline-alkali soil to balance the pH value of the soil and mineralize the salt content in the soil, thereby improving the tillability of the saline-alkali soil and greatly enhancing the crop yield and seedling survival percent.

The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises a composition of at least six or seven strains from bacillus subtilis, bacillus megatherium, pseudomonad, sphingobacterium thalpophilum, candida tropicalis, bacillus firmus and lactobacillus plantarum. The application includes sludge treatment.

The invention relates to the field of bioremediation of rivers, and in particular relates to a composite microbial preparation for watershed bioremediation as well as a preparation method and application thereof. The microbial preparation comprises active ingredients including bacillus natto, candida utilis and rhodopseudomonas palustris which are mixed together in a volume fraction ratio of 1:1:1; the microbes for water treatment are combined with zeolite to prepare a comprehensive preparation for water treatment; the specific gravity of the obtained comprehensive preparation for water treatment is greater than that of water, so that the obtained comprehensive preparation for water treatment quickly sinks to the bottom of water and can not be washed away by water flow easily; the zeolite is used as a carrier for the microbes to grow, and can provide certain nutrition substances required by growth for the microbes while adsorbing pollutants, and the microbes attached to the zeolite perform decomposition and anabolism on the pollutants to ensure that the zeolite restores the adsorptive property, so that a virtuous cyclic process is formed. The microbial agent provided by the invention can adapt to fresh water and seawater environments at the same time, and has an effective bioremediation effect on watercourses of certain coastal cities, wherein the water quality of the watercourses has fresh water and seawater alternating conditions along with seasonal changes.

The invention discloses a universal feed starter composed of the following raw materials by weight: 20-30 parts of soybean meal, 20-30 parts of corn, 20-30 parts of straw, 10-20 parts of fish meal, 10-20 parts of bran, 5-10 parts of sugar, 5-10 parts of Omega-3 fatty acid, 100 parts of multidimensional composite enzyme bacteria and 50-100 parts of water; the multidimensional composite enzyme bacteria contains 5-10 parts of bcillus subtilis, 5-1 parts of bacillus licheniformis, 10-20 parts of acetobacter aceti, 10-20 parts of lactobacillus amylovorus, 5-10 parts of candida, 10-20 parts of protease, 5-10 parts of geotrichum candidum, and balance of enterococcus faecalis. The efficient feed starter prepared by fermentation of various raw materials by the multidimensional composite enzyme bacteria can be widely used in breeding of ruminant animals, swine, poultry, aquatics and the like. The efficient feed starter fermentation agent belongs to a micro ecological preparation, does not contain any chemical agents and hormones, and is used in the production of green livestock products and aquatic products beneficial to human health.

The invention relates to a method for producing a compound microorganism phosphate fertilizer using a low grade rock phosphate powder which comprises the following steps: 1) preparing a first bacteria solution of candidakrissii and a first bacteria solution of acidithiobacillus ferrooxidans; 2) preparing a secondary bacteria solution of candidakrissii and a secondary bacteria solution of acidithiobacillus ferrooxidans; 3) preparing the compound microorganism phosphate fertilizer accoring to the following weight percentage: (1) rice, wheat or corn stalk 20-30%, (2) ammonium sulphate 5-10%, (3) pyrite powder(200-300 mesh) 10-20%, (4) lowgrade phosphate rock(200-300 mesh) 10-20%, (5) the secondary bacteria solution of candidakrissii 15-20%, (6) the secondary bacteria solution of acidithiobacillus ferrooxidans 15-20%. The method has advantage of low cost and can improve the phosphate fertilizer utilization efficiency.

The invention relates to a biological agent for glutamate wastewater treatment. The biological agent is formed by mixing aerobic denitrifier, bacillus, aspergillus niger, rhodococci, alcaligenes faecalis, streptomyces lateritius, Candida albicans and an adsorbent carrier. The biological agent contains various microorganisms with the good degradation ability to non-biodegradable pollutants, all the bacteria are reasonably matched, the good degradation effect is achieved, and the wide application prospect is achieved.

Food containing γ-glutamylcysteine or cysteine is produced by culturing Candida utilis containing 1% by weight or more of γ-glutamylcysteine per dry cells in logarithmic growth phase when cultured in a minimal medium, for example, Candida utilis in which a gene encoding glutathione synthetase is modified so that intracellular glutathione synthetase activity is reduced, under a suitable condition and mixing the obtained culture or a fraction thereof or the culture or a fraction thereof subjected to heat treatment with a raw material of food or drink to process food or drink.

The invention provides a nucleotide sequence suitable for secretory expression of recombinant candida utilis uricase (uricase, EC1.7.3.3) by virtue of escherichia coli, a carrier containing the nucleotide sequence, an engineering cell containing the carrier, and a method for purifying the recombinant candida utilis uricase from the engineering cell. On the basis of a natural sequence of a uricase gene, a codon of the uricase gene is optimized, the transformed uricase gene is cloned to a prokaryotic expression vector pET42a(+); an escherichia coli Rossetta strain is transformed; the expression level can reach 50% of soluble protein of the escherichia coli by IPTG induction; the expressed protein is soluble protein; and the biological activity of the expressed protein can be up to that of 37.89U / mg recombinant protein. The recombinant candida utilis uricase product can be efficiently and simply obtained at low cost. The invention also comprises an application of the recombinant candida utilis uricase prepared by the method in preparation of medicines, foods, health care products and cosmetics for treating hyperuricemia-related diseases or pathological symptoms of a human body.

There is disclosed a yeast strain of Candida utilis, wherein the yeast strain has been transformed with at least one copy of a gene that is operatively linked to a promoter sequence enabling expression of the gene and encodes a polypeptide having activity of lactate dehydrogenase. The yeast strain is useful for producing lactic acid with high efficiency.

The invention relates to a fungus agent for reducing mildew of fermentation feed and mycotoxin harm and application. The microbial agent for reducing mildew of fermentation feed and mycotoxin harm comprises the following effective components: Bacillus subtilis, Candida utilis, lactic acid bacteria, compound enzyme preparation, glucose and peptone. According to the present invention, Bacillus subtilis Bsbc01 and Candida utilis are used together for the first time to prepare the fungus agent, Candida utilis has the advantages of simple required nutrients, fast growth and reproduction, etc., canquickly consume the oxygen present in the feed gap and limit the growth and reproduction of mold, and at the same time, Candida utilis has the characteristics of high protein and vitamin B content, etc., which can increase the nutritional value of feed.

The invention relates to an inoculating culture and detecting method for gathering copper ions through saccharomycete. According to the inoculating culture method, at first, inoculating culture media with different copper ion concentrations and a basal culture medium are prepared; the inoculating culture media with the different copper ion concentrations are inoculated with a cultured candida utilis strain solution, and shaking culture is carried out; candida utilis with a high OD600 value and the low content of the copper ions in supernatant liquor is selected from the cultured strain solution to serve as starting strains; a starting strain solution is taken out, the inoculating culture medium with the smallest copper ion concentration is inoculated with the starting strain solution, after shake-flask culture is carried out, the strain solution is absorbed again, the inoculating culture medium with the second smallest concentration is inoculated with the strain solution, and inoculating culture is carried out according to the method; finally, copper ion conversion rates of different generations of the candida utilis are detected, and when the conversion rates of the copper ions are unchanged or reduced, the strains are strains for producing copper gathering yeast. The cultured saccharomycete can grow in the culture medium with the large copper ion concentration, the conversion efficiency of the copper ions is high, and the basis is provided for development of the new function of the yeast.

The invention provides a preparation method for a microbial garbage deodorizer. The preparation method comprises the following steps: 1, preparing lactobacillus plantarum fermentation liquor; 2, preparing a candida utilis fermentation liquor; 3, fully mixing the lactobacillus plantarum fermentation liquor and the candida utilis fermentation liquor, which are prepared in step (1) and step (2), according to a proportion of (1.5-2.0):1. According to the preparation method, odor of garbage can be effectively degraded, the production of other septic matters can be inhibited, and a function of remediating a garbage landfill in situ is achieved.

The invention relates to a method for preparing a novel biological microcapsule capable of being applied to treatment of ammonia nitrogen and soluble organic substances in water. According to the method for preparing the microcapsule, no chemical crosslinking agent or external material is added as a carrier, but candida is spontaneously combined to an extracellular polymer excreted by fungal hyphae cell aggregation in a natural mode under a special condition, and an appropriate condition is created to induce the intergrowth of hyphomycete and the candida so as to form the biological microcapsule in the shape of a solid microsphere. The novel biological microcapsule can efficiently degrade the ammonia nitrogen and the soluble organic substances in water, can keep the activity of thalli, separates the thalli from the outside poor environment, slows down the influence caused by temperature and pressure, and is favorable for uniform distribution in water, and also favorable for stability in storage and use. The preparation method is simple and feasible, has less influence factors, and has great potential and application value for treating the ammonia nitrogen and the soluble organic substances in the water.