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Preparation method for microbial garbage deodorizer

A technology for microorganisms and deodorants is applied in the field of preparation of garbage microbial deodorants, which can solve the problems of ecological environment impact, air pollution, harm to sanitation workers, etc., and achieve the effects of inhibiting physiological activity, good environmental protection performance and environmental improvement.

Active Publication Date: 2015-04-29
HUNAN KEMEIJIE ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The composition of garbage is complex, and a large amount of toxic and harmful odorous gases are produced during the process of collection, transfer, and stacking, which not only pollutes the air, but also affects the ecological environment, and also causes the spread of human and animal diseases, affecting the normal life and health of residents, and causing serious damage to the environment. Sanitation workers pose great hazards

Method used

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  • Preparation method for microbial garbage deodorizer
  • Preparation method for microbial garbage deodorizer
  • Preparation method for microbial garbage deodorizer

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preparation example Construction

[0029] see figure 1 , figure 2 , image 3 The preparation method of shown rubbish microorganism deodorant, its step is as follows:

[0030] (1) Prepare Lactobacillus plantarum fermentation liquid;

[0031] (2) preparing Candida utilis fermentation broth;

[0032] (3) Fully mix the Lactobacillus plantarum fermentation broth prepared in step (1) and step (2) and the Candida utilis fermentation broth in a volume ratio of 1.5-2.0:1.

[0033] The preparation steps of the plantarum lactobacillus fermented liquid are as follows:

[0034] a1, strain activation, is to place Lactobacillus plantarum in the MRS medium and obtain activated fresh strain 1 through the activation of the slant strain; the MRS medium is the lactic acid bacteria fermentation medium;

[0035] a2. Seed bottle cultivation is to inoculate the seed liquid culture medium in the seed bottle with the fresh strains activated in step a1, and after cultivating at a constant temperature of 37°C for 28 ...

Embodiment 1

[0045] 1. Preparation of Lactobacillus plantarum fermentation product

[0046]Activation of slant strains: take the refrigerator to store the slant strains of plant lactic acid bacteria, inoculate them into fresh test tube MRS medium by aseptic operation, cultivate them on the slant, and cultivate them at a constant temperature of 37°C overnight to make fresh test tube slant strains. The MRS medium: by volume, peptone, 1%; beef extract, 1%; yeast extract, 0.5%; diammonium hydrogen citrate, 0.2%; glucose, 2%; Tween 80, 0.1%; sodium acetate , 0.5%; dipotassium hydrogen phosphate, 0.2%; magnesium sulfate, 0.058%; manganese sulfate, 0.025%; agar, 1.5% to 2.0%; the rest is water; pH 6.2 to 6.6.

[0047] Seed bottle culture: Take one ring of activated fresh test tube slant bacteria, inoculate it into the seed liquid medium in the seed bottle, the filling volume is 250 mL / 500 mL, after 28 hours of constant temperature cultivation at 37°C, stop the cultivation, and make the inoculum ...

Embodiment 2

[0057] 1. Preparation of Lactobacillus plantarum fermentation product

[0058] Activation of slant strains: take the refrigerator to store the slant strains of plant lactic acid bacteria, inoculate them into fresh test tube MRS medium by aseptic operation, cultivate them on the slant, and cultivate them at a constant temperature of 37°C overnight to make fresh test tube slant strains. The MRS medium: peptone by volume, 1%; beef extract, 1%; yeast extract, 0.5%; diammonium hydrogen citrate, 0.2%; glucose, 2%; Tween 80, 0.1%; sodium acetate, 0.5%; dipotassium hydrogen phosphate, 0.2%; magnesium sulfate, 0.058%; manganese sulfate, 0.025%; agar, 1.5% to 2.0%; the rest is water; pH 6.2 to 6.6.

[0059] Seed bottle culture: take one ring of activated fresh test tube slant bacteria, inoculate it into the liquid medium in the seed bottle, the filling volume is 250 mL / 500 mL, after 28 hours of constant temperature cultivation at 37°C, stop the cultivation, and make the inoculum; The l...

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Abstract

The invention provides a preparation method for a microbial garbage deodorizer. The preparation method comprises the following steps: 1, preparing lactobacillus plantarum fermentation liquor; 2, preparing a candida utilis fermentation liquor; 3, fully mixing the lactobacillus plantarum fermentation liquor and the candida utilis fermentation liquor, which are prepared in step (1) and step (2), according to a proportion of (1.5-2.0):1. According to the preparation method, odor of garbage can be effectively degraded, the production of other septic matters can be inhibited, and a function of remediating a garbage landfill in situ is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method of a garbage microbial deodorant. Background technique [0002] According to incomplete statistics, the growth rate of garbage in my country has reached more than 10%, and nearly 150 million tons are produced every year. At present, domestic municipal solid waste has accumulated as much as 7 billion tons, covering an area of ​​more than 800,000 mu, of which 80% to 90% come from large and medium-sized cities. Only about 50%. The composition of garbage is complex, and a large amount of toxic and harmful odorous gases are produced during the process of collection, transfer, and stacking, which not only pollutes the air, but also affects the ecological environment, and also causes the spread of human and animal diseases, affecting the normal life and health of residents, and causing serious damage to the environment. Sanitation work...

Claims

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Application Information

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IPC IPC(8): A61L9/01A61L101/52
Inventor 李荣
Owner HUNAN KEMEIJIE ENVIRONMENTAL PROTECTION TECH CO LTD
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