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Method for producing composite microorganism phosphate using low grade ground phosphate rock

A technology of compounding microorganisms and phosphate rock powder is applied in fertilizer mixtures, applications, organic fertilizers, etc., which can solve the problems of increasing production costs, harshness, and requirements for microbial culture conditions, and achieve the effects of reducing production costs and improving the utilization rate of phosphate fertilizers.

Inactive Publication Date: 2008-12-24
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are a small number of related invention patents at home and abroad on the use of microbial leaching technology to process phosphate rock to produce microbial phosphate fertilizers. increased production costs
In addition, currently there are basically no invention patents specifically aimed at the development and utilization of low-grade phosphate rock resources. If chemoheterotrophic microorganisms and chemoautotrophic microorganisms can be used comprehensively to process low-grade phosphate rock to produce compound microbial phosphate fertilizers, both Make up for the lack of related invention patents before, improve the efficiency of phosphate fertilizers, and at the same time enable my country's rich low-grade phosphate rock resources to be fully developed and utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A kind of method utilizing low-grade phosphate rock powder to produce compound microbial phosphate fertilizer, it comprises the steps:

[0064] 1), ① Inoculate 10 mL of Candida krissii (Candida krissii) into 90 mL of Candida krissii primary culture medium (with a volume concentration of 20% sulfuric acid in advance) through a pipette in an ultra-clean workbench. Adjust the pH of the Candida crescendo primary medium to 2.5), place it in a constant temperature shaker for shaking culture, the rotation speed is 180 rpm, and the culture temperature is 30°C. After cultivating for 6 days, filter and collect the filtrate, which is cress Candida primary bacterial liquid;

[0065] Described Candida crissa primary culture medium (NBRIP medium) component:

[0066] Glucose 10g,

[0067] (NH 4 ) 2 SO 4 0.15g,

[0068] KCl 0.2g,

[0069] MgCl 2 ·6H 2 O 0.5g,

[0070] MgSO 4 ·7H 2 O 0.25g,

[0071] Ca 3 (PO 4 ) 2 5g,

[0072] Distilled water 1000mL; ...

Embodiment 2

[0112]A kind of method utilizing low-grade phosphate rock powder to produce compound microbial phosphate fertilizer, it comprises the steps:

[0113] 1), ① inoculate 15mL of Candida krissii (Candida krissii) into 100mL of Candida krissii primary culture medium through a pipette in the ultra-clean workbench (first use 20% sulfuric acid with a volume concentration Adjust the pH of the Candida crescendo primary medium to 2.8), place it in a constant temperature shaker for shaking culture, the rotation speed is 160 rpm, and the culture temperature is 32°C. After cultivating for 5 days, filter and collect the filtrate, which is cress Candida primary bacterial liquid;

[0114] Described Candida crissa primary culture medium (NBRIP medium) component:

[0115] Glucose 10g,

[0116] (NH 4 ) 2 SO 4 0.15g,

[0117] KCl 0.2g,

[0118] MgCl 2 ·6H 2 O 0.5g,

[0119] MgSO 4 ·7H 2 O 0.25g,

[0120] Ca 3 (PO 4 ) 2 5g,

[0121] Distilled water 1000mL;

[0122] ② Inoc...

Embodiment 3

[0161] A kind of method utilizing low-grade phosphate rock powder to produce compound microbial phosphate fertilizer, it comprises the steps:

[0162] 1) Inoculate 20mL of Candida krissii (Candida krissii) into 150mL of Candida krissii primary culture medium through a pipette in an ultra-clean workbench (first adjust the creatinine with a volume concentration of 10% sulfuric acid The pH of Candida stuniscens primary medium is 3.0), placed in a constant temperature shaker for shaking culture, the rotation speed is 170 rpm, and the culture temperature is 28°C. After 4 days of culture, filter and collect the filtrate, which is Candida crescendo The primary bacterial liquid;

[0163] Described Candida crissa primary culture medium (NBRIP medium) component:

[0164] Glucose 10g,

[0165] (NH 4 ) 2 SO 4 0.15g,

[0166] KCl 0.2g,

[0167] MgCl 2 ·6H 2 O 0.5g,

[0168] MgSO 4 ·7H 2 O 0.25g,

[0169] Ca 3 (PO 4 ) 2 5g,

[0170] Distilled water 1000mL;

...

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PUM

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Abstract

The invention relates to a method for producing a compound microorganism phosphate fertilizer using a low grade rock phosphate powder which comprises the following steps: 1) preparing a first bacteria solution of candidakrissii and a first bacteria solution of acidithiobacillus ferrooxidans; 2) preparing a secondary bacteria solution of candidakrissii and a secondary bacteria solution of acidithiobacillus ferrooxidans; 3) preparing the compound microorganism phosphate fertilizer accoring to the following weight percentage: (1) rice, wheat or corn stalk 20-30%, (2) ammonium sulphate 5-10%, (3) pyrite powder(200-300 mesh) 10-20%, (4) lowgrade phosphate rock(200-300 mesh) 10-20%, (5) the secondary bacteria solution of candidakrissii 15-20%, (6) the secondary bacteria solution of acidithiobacillus ferrooxidans 15-20%. The method has advantage of low cost and can improve the phosphate fertilizer utilization efficiency.

Description

technical field [0001] The invention discloses a method for producing compound microbial phosphate fertilizer by using low-grade phosphate rock powder. Background technique [0002] Phosphorus is one of the most important nutrients for plants, and phosphorus fertilizers have long played an important role in increasing crop yields. The production of traditional phosphate fertilizers is generally made by dissolving phosphate rock powder with inorganic acids such as sulfuric acid, and chemically fermenting the phosphate rock powder with sulfuric acid. However, the utilization rate of phosphorus fertilizer produced by this method is very low, and more than 80% of the phosphorus element is not absorbed by the crops, but is fixed by the soil, thus causing a great waste of resources and an increase in the production of wood, and also caused huge losses. environmental pollution. [0003] my country is a country with a large amount of phosphate rock resources, but most of them are ...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F11/08C05B17/00C12N1/16
Inventor 池汝安肖春桥
Owner WUHAN INSTITUTE OF TECHNOLOGY
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