183 results about "Trichoderma asperellum" patented technology

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses a biological prevention and control bacterial strain for preventing and controlling rice sheath blight, a biological organic fertilizer, and a preparation method of the biological organic fertilizer, which relate to biological prevention and control bacterial strains of rice and biological organic fertilizers thereof, and belong to the technology of intensive agricultural production. According to the invention, Trichodermaasperellum12, which plays an obvious role in biological prevention and control on the rice sheath blight, is obtained by separation, the biological organic fertilizer is prepared by composting the biological prevention and control bacterial strain, rice hulls, straws and poultry excrement and contains bacterial strain hypnospore of more than 0.5*10<8>cfug<-1>, the water content of the fertilizer is smaller than 15% and the organic content of the fertilizer is more than 35%. Experiments show that after the biological organic fertilizer is applied into rice fields, most of matter in the organic fertilizer can keep afloat, the hypnospore of the biological prevention and control bacterial strain, which is adsorbed on the organic fertilizer, can rapidly breed on water, aerial mycelium is formed at a water-gas junction zone, and a hypha belt is formed at the contact part of rice stalks and the water surface, so that rhizoctonia solani of the rice sheath blight on the water surface is effectively prevented from contacting the rice stalks, and the prevention and control rate of the rice sheath blight is more than 70%.

The invention discloses a screening method and use of trichoderma asperellum. The method comprises: collecting an arsenic-contaminated soil sample, separating fungi from the soil sample in a laboratory, culturing the fungi at different arsenic concentrations, observing the growing conditions and arsenic resistances of the fungus colonies, and thus, screening trichoderma asperellum. The trichoderma asperellum screened by the method can be used to prepare corresponding microbial agents; when the prepared microbial agent is used in contaminated soil or environment, through the enrichment and volatilization functions of the trichoderma asperellum, the arsenic content in soil is lowered to a certain extent, and particularly the effective arsenic content in the soil is lowered; and thus, the normal growth of the crops is ensured, and the arsenic content in the crops and the agricultural products reaches the requirements for pollution-free agricultural products.

The invention provides Trichoderma asperellum with the antagonistic action on banana wilt and application of the Trichoderma asperellum. The Trichoderma asperellum with the antagonistic action on banana wilt is Trichoderma asperellum PZ6 which is preserved in the China Center for Type Culture Collection on January 18th, 2016 with the preservation serial number of CCTCC No: M 2016043 at the address of Wuhan University in Wuhan, China. As separated from plant rhizosphere soil, the Trichoderma asperellum PZ6 is a soil inhabitant and is high in rhizosphere colonization capability, good in control effect and especially high in antagonistic action on banana wilt pathogenic bacteria; besides, the culture conditions are simple, preservation and industrial production are easy, and good development and application prospects are achieved.

The invention belongs to the technical field of microbial control, and particularly relates to trichoderma asperellum and application thereof. Research results show that the trichoderma asperellum T0206 strain in the invention has good effects on inhibition of a variety of pathogenic bacteria such as fusarium wilt pathogenic bacteria, inhibition of diseases such as cucumber fusarium wilt, and degradation of phenolic acid substances; and the multifunctional biocontrol microorganism with disease prevention capability and phenolic acid substance degradation capability and the microbial preparation of the multifunctional biocontrol microorganism have important significance on relieving crop continuous cropping obstacles caused by fusarium wilt and phenolic acid substance stress and improving the crop yield.

The invention belongs to the field of microbe fertilizers, and provides a micro-ecological composite inoculant special for overcoming watermelon continuous cropping obstacle and an application method so as to solve the problems that various method for overcoming watermelon continuous cropping obstacle possess responding defects. The inoculant is prepared by mixing bacillus laterosporus, bacillus amyloliquefaciens, bacillus circulans, propionibacterium shermanil, tree microbacterium sp., bacillus laevolacticus, trichoderma harzianum, trichoderma asperellum, trichoderma fasciculatum Bissett, paecilomyces lilacinus, streptomyces syringini, gordonia sp., tsukamurella sp. and conexibacter sp. according to a certain ratio. The composite inoculant guarantees watermelon quality safety and environment zero pollution, and helps to reduce peasant production cost, guarantee agricultural product quality safety and protect ecological environment. The composite inoculant effectively overcomes watermelon continuous cropping obstacle, and watermelon which is truly nuisance-free, pure in mouthfeel and high in sweetness is produced. The composite inoculant possesses significant meaning on watermelon production, watermelon peasant income increase, consumer health guarantee, environment pollution reduction and maintenance of agricultural sustainable development.

The invention relates to a pasty biocontrol preparation for strawberry greensickness, and a preparation method, application and a special strain thereof. The classification designation of the special strain is Trichoderma asperellum GY20, and the collection number is CGMCC NO:4899. The pasty biocontrol preparation provided by the invention takes hyphae and chlamydospores of the Trichoderma asperellum GY20 as active components of the preparation. According to the invention, the pasty biocontrol preparation has the biggest advantage of stable product quality, and can be stored for 18 months at room temperature and 3 years under refrigeration conditions; the pasty biocontrol preparation has favorable control effect on strawberry greensickness; and waste water, waste gas and waste residues are never generated, thereby being beneficial to environmental protection.

The invention discloses trichoderma and application. The trichoderma is Trichoderma asperellum; the strain number is HTTA-Z0002; the preservation number is CGMCC No.15677. The trichoderma can be applied to plant disease prevention and treatment, bacteriostat and pesticide. The strain has stable biological features and pesticide activity; the culture is easy; the culture period is short; the operation method is simple, convenient and feasible; great improvement is realized in aspects of characteristics of bacteriostatic activity, spore generation through liquid culture and the like through being compared with that of wild strains; the defect of small spore production quantity of the trichoderma strain liquid culture is overcome; no pollution is caused on the farm products and environment; wide and strong inhibition effects are realized on various pathogenic bacteria of plants.

The present invention discloses new trichoderma asperellum and use thereof. The trichoderma asperellum is trichoderma asperellum LB-N2 preserved in China General Microbiological Culture Collection Center, and the accession number is CGMCC No. 3.17461. Metabolites of the trichoderma asperellum have activity for inhibition of escherichia coli, salmonella staphylococcus aureus and other bacteria, can be used for preparing anti-bacterial drugs or disinfectants, and have good prospects for clinical application.