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Trichoderma asperellum with antagonistic action on banana wilt and application of Trichoderma asperellum

A technology for banana fusarium wilt and Trichoderma aculeatus, applied in the field of microorganisms, can solve the problems of no control measures, isolation and waste, and no control of banana wilt, etc., achieve good control effect, easy preservation, and good development and application prospects Effect

Pending Publication Date: 2016-11-09
广西壮族自治区农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2002, Fusarium wilt race 4 was found in alpine banana orchards in the banana export base of the Philippines. Except for isolation and abandonment, there is still no other good control method
So far, no effective method for the prevention and treatment of banana fusarium wilt has been found at home and abroad. Once discovered abroad, the diseased area is declared as an epidemic area

Method used

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  • Trichoderma asperellum with antagonistic action on banana wilt and application of Trichoderma asperellum
  • Trichoderma asperellum with antagonistic action on banana wilt and application of Trichoderma asperellum
  • Trichoderma asperellum with antagonistic action on banana wilt and application of Trichoderma asperellum

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Effect test

Embodiment 1

[0037] Trichoderma aculeatus PZ6 in the present embodiment is a strain of Trichoderma screened and isolated from the banana garden soil in Dacheng Town, Pubei County, Qinzhou City, Guangxi. This strain has a strong effect on Fusarium oxysporum f.sp.cubense. It was deposited in China Center for Type Culture Collection on January 18, 2016, with the preservation number CCTCC No: M2016043, and was identified as Trichoderma asperellum PZ6 (Trichoderma asperellum PZ6).

[0038] The isolation and screening process of strain PZ6 is as follows:

[0039]Weigh 10g of the collected rhizosphere soil, dilute it with sterile water to an appropriate multiple, spread it on the Bengal red medium, and cultivate it at 28°C for about 5 days. Insert one or more indicator bacteria into the fungus cake of Fusarium wilt. When a certain fungus has an antagonistic effect, the indicator bacteria cannot grow beyond the fungus and present an obvious antagonistic zone. Dilution method" for isolation and pu...

Embodiment 2

[0046] Solid microbial agent and its preparation method.

[0047] The solid bacterial agent containing the above-mentioned Trichoderma aculeatus PZ6 provided by the present embodiment is prepared by the following method:

[0048] (1) Strain activation: Trichoderma aculeatus PZ6 was inoculated on PDA solid medium and cultured for 3-4 days.

[0049] (2) Seed culture: the activated bacterial strain is inoculated into the liquid medium, and the formula is: glucose 1%, potato juice 2%, by mass percentage, the rest is water; the pH value is in a natural state; 120rpm shake flask fermentation culture for 72 hours.

[0050] (3) Solid culture: mix the seed culture liquid with the solid medium, the formula of the solid medium is: 90% of wheat bran, 8-9% of corn flour, 1-2% of rice bran, calculated by mass percentage. The medium used in this example is the preferred medium, and other mediums are also acceptable, and there is no special limitation; the culture time is about 5 days at 28...

Embodiment 3

[0053] Field control effect of solid bacterial agent on banana fusarium wilt:

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Abstract

The invention provides Trichoderma asperellum with the antagonistic action on banana wilt and application of the Trichoderma asperellum. The Trichoderma asperellum with the antagonistic action on banana wilt is Trichoderma asperellum PZ6 which is preserved in the China Center for Type Culture Collection on January 18th, 2016 with the preservation serial number of CCTCC No: M 2016043 at the address of Wuhan University in Wuhan, China. As separated from plant rhizosphere soil, the Trichoderma asperellum PZ6 is a soil inhabitant and is high in rhizosphere colonization capability, good in control effect and especially high in antagonistic action on banana wilt pathogenic bacteria; besides, the culture conditions are simple, preservation and industrial production are easy, and good development and application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Trichoderma aculeatus antagonizing banana wilt and its application. Background technique [0002] Banana, a large herbaceous plant of the genus Musa of the Musaceae (Musaceae), also refers to its fruit. It is widely cultivated and eaten in tropical areas. It is native to southeastern Asia and is cultivated in Taiwan, Hainan, Guangdong, Guangxi and other regions. Bananas are fragrant and nutritious. [0003] Banana Fusarium wilt, also known as Panama disease and yellow leaf disease, is a devastating banana fungal disease caused by Fusarium oxysporum f.sp.cubense (Foc). Typical soil borne disease. The disease has a wide transmission route, strong pathogenicity, fast spread, high infection rate, difficult control, and high mortality of plants after infection. Once the banana wilt occurs, it will be extremely destructive and will seriously harm the banana in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 覃柳燕李朝生韦绍龙黄素梅周维田丹丹孙嘉曼龙盛风韦弟张进忠杨柳韦莉萍
Owner 广西壮族自治区农业科学院生物技术研究所
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