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Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof

A biological control agent, Verticillium wilt technology, applied in the field of microorganisms, to achieve good control effect, large profit margins, and stable quality

Active Publication Date: 2013-04-03
JIANGSU GENGYUN CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no relevant report on the control of strawberry Verticillium wilt with Trichoderma asperellum and its ointment biocontrol agents

Method used

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  • Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof
  • Pasty biocontrol preparation for strawberry greensickness, and preparation method, application and special strain thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] Specifically, the preparation method of the pasty biocontrol preparation prepared by preventing and treating strawberry Verticillium dahliae strain GY20 of the present invention comprises the following steps:

[0044] (A) Obtaining the bacterial solution

[0045] Use a sterile inoculation knife to pick out the GY20 spores that have been cultivated on the potato dextrose agar medium (PDA) in advance, and wash them with sterile water to adjust the spore content to 10. 5 The spore / mL inoculum was inoculated into a 750 ml Erlenmeyer flask containing 200 ml potato grape liquid medium (PD) at 5% (V / V) inoculation volume, at 26 °C and 150 rpm / min Shake culture for 48 h, inoculate 200 ml aliquots of the culture solution obtained in the previous step into 4 Erlenmeyer flasks with a volume of 2000 ml filled with 500 ml PD culture solution, shake and culture at 150 rpm and 26°C for 48 h; 2000ml of culture medium was inoculated into a 30L fermenter (Zhenjiang Dongfang GUS-30) cont...

Embodiment 1

[0051] Embodiment 1 Paste biocontrol preparation and preparation method thereof

[0052] The components of the pasty biocontrol preparation are:

[0053] Active ingredient [Trichoderma aculeatus ( Trichoderma asperellum ) GY20 mycelium and chlamydospore mixture] 94.999%

[0054] Copper ion inhibitor MDA-5 0.001%

[0055] Potato starch 5%

[0056] pH 3

[0057] The percentages mentioned above are percentages by weight.

[0058] The preparation method of above-mentioned pasty biocontrol preparation comprises the following steps:

[0059] (A) Obtaining the bacterial solution

[0060] Use a sterile inoculation knife to pick out the GY20 spores that have been cultivated on the potato dextrose agar medium (PDA) in advance, and wash them with sterile water to adjust the spore content to 10. 5 The spore / mL inoculum was inoculated into a 750 ml Erlenmeyer flask containing 200 ml potato grape liquid medium (PD) at 5% (V / V) inoculation volume, at 26 °C and 150 rpm / min Shake cultu...

Embodiment 2-3

[0064] Examples 2-3 were prepared according to the method of Example 1, and the differences are shown in Table 1.

[0065]

[0066] Components / Examples of Paste Biocontrol Preparations Example 2 Example 3 active ingredient 89.95% 91.99% Copper ion inhibitor MDA-5 0.05% 0.01% potato starch 10% 8% pH value 4.0 3.5

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PUM

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Abstract

The invention relates to a pasty biocontrol preparation for strawberry greensickness, and a preparation method, application and a special strain thereof. The classification designation of the special strain is Trichoderma asperellum GY20, and the collection number is CGMCC NO:4899. The pasty biocontrol preparation provided by the invention takes hyphae and chlamydospores of the Trichoderma asperellum GY20 as active components of the preparation. According to the invention, the pasty biocontrol preparation has the biggest advantage of stable product quality, and can be stored for 18 months at room temperature and 3 years under refrigeration conditions; the pasty biocontrol preparation has favorable control effect on strawberry greensickness; and waste water, waste gas and waste residues are never generated, thereby being beneficial to environmental protection.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a paste-like biocontrol preparation for strawberry verticillium wilt, [0002] Preparation method, application and special bacterial strain thereof. Background technique [0003] Strawberry (Fragaria ananassa) belongs to the Rosaceae (Rosaceae) strawberry genus perennial herb. At present, the annual output of strawberries in the world has exceeded 5 million tons, and its output ranks first in the production of small berries in the world. In recent years, strawberry cultivation in greenhouses has gradually emerged. , the planting area is gradually expanding, but there are contradictions between crop rotation and fixed facilities. Continuous cropping obstacles are becoming more and more obvious. Strawberry continuous cropping diseases are the main factors restricting strawberry production at present, especially strawberry Verticillium wilt, which is widespread in strawberry producing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/00A01P3/00C12R1/885A01N63/38
CPCA01N25/00C12N1/14A01N63/04C07K14/37A01N63/38A01N63/30A01N25/04
Inventor 李楠张海军罗志会刘慕兰李惠
Owner JIANGSU GENGYUN CHEM CO LTD
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