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New trichoderma asperellum and use thereof

A technology of Trichoderma aculeatus and LB-N2, which can be applied in application, microbial-based method, and resistance to vector-borne diseases, etc., can solve the problems such as the inhibition of bacteria by Trichoderma aculeatus, and achieve the effect of good clinical application prospect.

Active Publication Date: 2016-06-22
GANZI TIBETAN AUTONOMOUS PREFECTURE INST OF ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no reports of Trichoderma aculeatus and its metabolites having antibacterial activity

Method used

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  • New trichoderma asperellum and use thereof
  • New trichoderma asperellum and use thereof
  • New trichoderma asperellum and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Isolation and identification of Trichoderma aculeatus of the present invention

[0027] 1. Separation

[0028] 1.1 Conventional endophytic fungi isolation method

[0029] Cut the middle part of the leaves of healthy Ligula spp. leaves into 2mm small pieces, soak the small piece with tweezers in 75% alcohol for 30S, rinse with sterile water for 2-3 times, then immerse in 75% alcohol for 30S, rinse with sterile water for 2-3 minutes 2 to 3 times, use the last sterile water as a control, take out the small leaves with sterile tweezers and stick them flat on the PDA plate, place 6 small pieces in each plate, seal with parafilm and place it at room temperature for 5 days, then continuously observe and count. The colony shape and quantity of the control group, if no bacteria grow in the control group, it means that the surface disinfection is complete.

[0030] 1.2 Purification of endophytic fungi

[0031] Pick three typical strains and plant them on double-an...

Embodiment 2

[0047] Embodiment 2 adopts bacterial strain fermentation of the present invention to prepare antibacterial metabolite

[0048] 1. Experimental materials

[0049]PDA solid medium (1L): 200 grams of potatoes, 20 grams of glucose, 15 to 20 grams of agar, and the rest is water; preparation method: weigh 200 grams of potatoes and cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, Just puncture the glass rod), filter with eight layers of gauze, add agar, continue to heat and mix well, after the agar is dissolved, add glucose, stir evenly, and then add water to 1000 ml after cooling down slightly.

[0050] PD liquid medium (1L): 200 grams of potatoes, 20 grams of glucose, and the rest is water; preparation method: weigh 200 grams of potatoes, cut them into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod) ), filter with eight layers of gauze, add glucose, stir evenly, cool down slightly, and then add...

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Abstract

The present invention discloses new trichoderma asperellum and use thereof. The trichoderma asperellum is trichoderma asperellum LB-N2 preserved in China General Microbiological Culture Collection Center, and the accession number is CGMCC No. 3.17461. Metabolites of the trichoderma asperellum have activity for inhibition of escherichia coli, salmonella staphylococcus aureus and other bacteria, can be used for preparing anti-bacterial drugs or disinfectants, and have good prospects for clinical application.

Description

technical field [0001] The invention relates to a new Trichoderma aculeatus and its application. Background technique [0002] Trichoderma (Trichoderma) fungi belong to the subphylum Deuteromycota, Hyphospora, Hyphospora, and Myxospora. It is a kind of fungus that is ubiquitous in nature. It is often found on plant residues and animal feces, and can often be isolated from the root circumference of plants and the surface of seeds and bulbs. Trichoderma has a wide range of control effects on plant pathogenic fungi, and Trichoderma has antagonistic effects on at least 29 important plant pathogenic fungi such as Pythium, Fusarium, Rhizoctonia, Sclerotinia, and Sclerotinia. At present, it has been widely used to prevent and control a variety of plant diseases caused by fungi, especially against seedling blight, shatter, etc. caused by Rhizoctonia solani, Fusarium spp. Soil-borne diseases such as infestation, canker sores, and phytophthora have good control effects. [0003] Tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A61K36/06A01N63/04A61P31/04A01P1/00C12R1/885
CPCY02A50/30
Inventor 刘曦胡延春罗彪蒋忠荣洛绒志玛龙兴发
Owner GANZI TIBETAN AUTONOMOUS PREFECTURE INST OF ANIMAL HUSBANDRY SCI
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