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Efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof

A technology of Trichoderma aculeatus, root rot, applied in the application, tobacco cultivation, biocide and other directions

Active Publication Date: 2021-05-28
TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the systematic biological control and biocontrol mechanism of tobacco Fusarium root rot

Method used

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  • Efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof
  • Efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof
  • Efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Isolation and screening of biocontrol fungi

[0039] Materials: In 2020, mainly in central Henan tobacco-growing areas, tobacco and surrounding plant rhizosphere soil microorganisms were collected from 7 cities and 14 counties in Xuchang, Luohe, Pingdingshan, Luoyang, Sanmenxia, ​​Zhumadian and Nanyang in 2020. Fusarium oxysporum, the main pathogen of tobacco Fusarium root rot, the pathogen of common tobacco diseases, and China Tobacco 100 seeds were all provided by the germplasm source bank of Tobacco Research Institute, Henan Academy of Agricultural Sciences.

[0040] Soil microorganisms were isolated and purified using potato dextrose agar (PDA).

[0041] Culture medium composition and use:

[0042]PDA: Dilute 39 g of potato dextrose agar to 1000 mL of distilled water, and sterilize at 121 °C for 15 min. (Or conventional medium formula: Weigh 200g of potatoes, wash, peel and cut into small pieces, add 1000ml of water and boil for 25 minutes until the sma...

Embodiment 2

[0052] Embodiment two: identification of biocontrol fungus

[0053] Refer to "Fungi Identification Handbook" and other relevant monographs and literature materials, and according to the screening results of bio-control fungi, select the purified strains to be tested, make water slides at different growth stages, and observe the mycelium morphology and sporulation mode under an optical microscope and the shape, size, and color of the fruiting body, and then preliminarily determine the taxonomic status of the strain according to the observed morphological characteristics of the plate.

[0054] Combined with the identification means of molecular biology, the genomic DNA of the strain to be tested was extracted using the Ezup Column Fungal Genomic DNA Extraction Kit (Shanghai Sangong), and the general primers for fungal identification ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4( 5′-TCCTCCGCTTATTGATATGC-3′) for PCR amplification. PCR amplification system (25 μL): DNA template 1 μL, ...

Embodiment 3

[0060] Example 3: Determination of Bacteriostatic Spectrum of Biocontrol Strains on Common Tobacco Diseases

[0061] Select the bio-control strain to be tested as the main target, and also adopt the plate confrontation culture method and the mycelial growth rate method to determine the effects on tobacco solani Fusarium, Phytophthora, root black rot, root rot, Phomopsis Inhibitory effect of 8 common tobacco diseases including genus, anthracnose, blight, and grape canker sores, the colony radius was measured, and the inhibition rate was calculated.

[0062] As shown in Table 2, except for Fusarium oxysporum, the Tr-0111 strain has different degrees of inhibitory effects on the pathogens of common tobacco diseases. Among them, the inhibitory effect on tobacco root black rot is the most significant, the inhibition rate is 90.11%, and the antagonism coefficient is grade I; the inhibitory effect on R. blight is relatively weak, the inhibition rate is 42.67%, and the antagonism coef...

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Abstract

The invention provides efficient biocontrol trichoderma asperellum for tobacco fusarium root rot and application thereof, the preservation name of the trichoderma asperellum is trichoderma asperellum Tr-0111, the trichoderma asperellum is preserved in the China Center for Type Culture Collection, the preservation date is August 21, 2020, and the preservation number is CCTCC NO: M 2020441; according to the research, soil microorganisms are separated and screened to obtain an efficient biocontrol fungus strain with a relatively good inhibiting effect on tobacco fusarium root rot, the variety of the strain is determined by utilizing a method of combining traditional morphology and modern molecular biology, and the growth promoting effect of the strain on tobacco is determined; a foundation is laid for further development of research on the control effect and bacteriostatic active substances of efficient biocontrol bacteria potted plants and productization and industrial production of biocontrol bacteria, and the efficient biocontrol trichoderma asperellum has certain scientific basis and application value for biological control of soil-borne diseases in main tobacco-growing areas in Henan province.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a high-efficiency biocontrol of Trichoderma aculeatus against Fusarium tobacco root rot and an application thereof. Background technique [0002] The National Academy of Sciences defined biological control in 1987 as: the use of natural or modified organisms, genes or gene products to reduce the action of pests to the benefit of beneficial organisms. Biocontrol fungi are defined as: natural or modified fungi whose cells, genes or gene products can be used to reduce harmful organisms and benefit beneficial organisms. According to the different objects of biocontrol fungi, they can be divided into insecticidal fungi, herbicidal fungi, and bactericidal fungi. [0003] Tobacco fusarium root rot has gradually increased in the range and degree of incidence in my country's tobacco-growing areas. It has occurred seriously in some counties (districts) in Yunnan, Guiz...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P21/00A01P3/00A01G13/00A01G22/45C12R1/885
CPCC12N1/14A01N63/38A01G13/00A01G22/45Y02A40/10
Inventor 李小杰邱睿白静科姚晨虓赵钧李成军刘畅陈玉国李静静李淑君李娟
Owner TOBACCO RES INST HENAN ACADEMY OF AGRI SCI
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