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Method for producing laccase by fermenting Shiraia bambusicola

A technology of bamboo yellow fungus and laccase, applied in the field of bioengineering, can solve problems such as no research report on bamboo yellow fungus, can not meet the enzyme amount, long fermentation period, etc., and achieves high medicinal value, short fermentation period and high health care. effect of efficacy

Inactive Publication Date: 2011-01-12
江苏竹红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although laccase is widely distributed in nature, there are not many strains with high laccase production, and the laccase production cannot meet the enzyme amount required for industrial production, and the fermentation period is long (7-14 days).
The strains currently used for laccase production mainly include: Phanerochaete chrysosporium (Phanerochaete chrysosporium ), Trametes versicolor ( Trametes versicolor ), Coriolus versicolor ( Coriolus hisutus ), versicolor versicolor ( Coriolus versicolor ), Microporosa hemorrhoids ( Pycnoporus sanguineus ) and Pleurotus ( Pleurotus otreatus ), etc., but no bamboo yellow fungus ( Shiraia bambusicola ) research report on the production of laccase

Method used

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  • Method for producing laccase by fermenting Shiraia bambusicola
  • Method for producing laccase by fermenting Shiraia bambusicola

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Experimental program
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Effect test

Embodiment 1

[0035] The composition of the medium is: carbon source 20g / L, potato 200g / L, peptone 20g / L, NH 4 NO 3 10g / L, corn syrup powder 12g / L, K 2 HPO 4 2 g / L, KH 2 PO 4 2 g / L, natural pH, 10% inoculum size, 24 hours seed age, 30 ℃ fermentation temperature, 300L airlift fermenter, 70% liquid volume, 1:1.5 v / v / m ventilation, The fermentation time is 3 days. Under these conditions, 8 batches of fermentation were carried out, and the carbon sources used in each batch of fermentation were different, which were glucose, fructose, soluble starch, maltose, xylose, sucrose, lactose, and galactose in sequence, and the concentration of each batch of carbon sources was 20g / L. The yields of laccase, SOD and perylenequinone pigments were as follows: figure 1 shown.

Embodiment 2

[0037] The composition of the medium is: glucose 20g / L, nitrogen source 30g / L, K 2 HPO 4 4 g / L, KH 2 PO 4 4 g / L, natural pH, 5% inoculum volume, 48 hours of seed age, 24 ℃ fermentation temperature, 300L airlift fermenter, 60% liquid volume, 1:1.5 v / v / m ventilation volume, The fermentation time is 3 days. Under these conditions, 9 batches of fermentation were carried out. The nitrogen sources used in each batch of fermentation were different, followed by ammonium chloride, ammonium nitrate, ammonium sulfate, urea, peptone, yeast extract, beef extract, corn steep liquor, soybean cake powder, and each batch of nitrogen source The concentration is 30g / L. The yields of laccase, SOD and perylenequinone pigments were as follows: figure 2 shown.

Embodiment 3

[0039] The composition of the medium is: glucose 20g / L, NH 4 NO 3 10g / L, KH 2 PO 4 4 g / L, peptone 10g / L, surfactant 0.08g / L, pH natural. The inoculum size was 10%, the seed age was 15 hours, the culture temperature was 28 °C, the 50L mechanically stirred fermenter, the liquid volume was 60%, the ventilation rate was 1:0.5 v / v / m, and the stirring speed was 150 r / min. The time is 3 days. When the fermentation starts, the surfactant is added to the fermentation broth immediately. Under this condition, 18 batches of fermentation were carried out, and the surfactants used in each batch of fermentation were different, which were Tween 20, Tween 40, Tween 60, Tween 80, Triton X-100, TritonX-114, TritonX-116, Triton X-405 , TX-4, TX-6, TX-9, TX-10, AEO-3, AEO-7, AEO-9, monoethanolamine, diethanolamine, triethanolamine, each batch of surfactant concentration is 0.08g / L. The yields of laccase, SOD and perylenequinone pigments were as follows: image 3 shown.

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Abstract

The invention relates to a method for producing laccase by fermenting Shiraia bambusicola, belonging to the technical field of bioengineering. The invention uses Shiraia bambusicola strain Shiraiasp.SUPER-H168 to obtain the laccase by liquid state fermentation, wherein the preservation number of the Shiraia bambusicola strain is CCTCCNO:M207104 which is published in ZL200710132510.4. The study on the laccase produced by Shiraia bambusicola is not reported. The Shiraia bambusicola is a medical fungus, which has high medicinal value and health efficacy. The method for producing the laccase by fermenting the Shiraia bambusicola has the advantages of short fermentation period and high enzyme activity, meanwhile, SOD with high activity and perylenequinonoid pigment can be produced in the fermentation process, and the development prospects are broad.

Description

technical field [0001] The invention discloses a method for fermenting and producing laccase from bamboo yellow fungus, which belongs to the technical field of bioengineering. The present invention relates to utilizing bamboo yellow bacterial strain ( Shiraia sp.) The SUPER-H168 preservation number is CCTCC NO: M 207104, which has been disclosed in ZL200710132510.4, and laccase is obtained through liquid fermentation. Background technique [0002] Laccase is a copper-containing polyphenol oxidase with a wide range of substrates. It can not only catalyze the oxidation of various aromatic compounds, but also degrade lignin and remove many toxic phenolic substances such as phenoxy herbicides. Toxicity, it can also decolorize a variety of dyes and remove the toxicity of petroleum industry wastewater; therefore, it has great research and application value in the fields of papermaking, environmental protection, food, medicine and hygiene, and biological detection. In recent years...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12R1/645
Inventor 蔡宇杰廖祥儒胡艳马文寅胡明明丁彦蕊李枝玲张大兵
Owner 江苏竹红生物科技有限公司
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