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A bamboo yellow fungus with high hypocretin production and its cultivation method and application

A technology of hypocrellin and bamboo yellow fungus, applied in the field of microbial fermentation, can solve the problem of low yield, achieve the effect of increasing the total amount of hypocrellin and efficiently producing hypocrellin

Active Publication Date: 2020-12-08
JINAN GUOLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although people have screened out some strains producing anthocyanin pigments and optimized their fermentation process, the yield is still generally low: Chinese invention patent CN101875905B discloses a strain of endophytic fungus from moso bamboo seeds, which is in a liquid state at 26°C. Yield after 144 hours of fermentation is about 0.17%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Seed cultivation

[0055] The bamboo yellow strain (Shiraiabambusicola) was subjected to seed culture: potato dipping powder 200g / L, sodium chloride 10g / L, fermentation temperature 26°C, rotation speed 200 rpm, cultured for 2 days.

[0056] (2) Fermentation culture

[0057] Adopting solid-state fermentation, the solid fermentation medium used consists of: rice 45%, bran 15%, NaCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, add water and mix to moisten, put into a jar, sterilize at 0.1MPa for 30-45min, add liquid seeds after cooling and mix well. The fermentation temperature was 26°C, and after 15 days of fermentation, the contents of hypocrellin A, hypocrellin B, hypocrellin C, and hypocrellin D in the fermentation medium were 543.mg / 100g, 710.2mg / 100g, 54.2mg / 100g, 9.2mg / 100g.

Embodiment 2

[0059] (1) Seed cultivation:

[0060] The bamboo yellow strain (Shiraiabambusicola) was subjected to seed culture: potato dipping powder 200g / L, sodium chloride 10g / L, fermentation temperature 26°C, rotation speed 200 rpm, cultured for 2 days.

[0061] (2) Fermentation culture

[0062] Solid fermentation is adopted, and the solid fermentation medium used is composed of: rice 45%, bran 15%, NaCl 0.5g / L, K 2 HPO 4 1%, MgSO4.7H 2 O 0.3%, add water and mix to moisten, put into a jar, sterilize at 0.1MPa for 30-45min, add liquid seeds after cooling and mix well. The fermentation temperature was 26°C, and after 15 days of fermentation, the contents of hypocrellin A, hypocrellin B, hypocrellin C, and hypocrellin D in the fermentation medium were 366mg / 100g, 554mg / 100g, and 103mg respectively / 100g, 7.7mg / 100g.

Embodiment 3

[0064] (1) Seed cultivation

[0065] The bamboo yellow strain (Shiraiabambusicola) was subjected to seed culture: potato dipping powder 200g / L, sodium chloride 10g / L, fermentation temperature 26°C, rotation speed 200 rpm, cultured for 2 days.

[0066] (2) Fermentation culture

[0067] Adopting solid-state fermentation, the solid fermentation medium used consists of: rice 45%, bran 15%, NaCl 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, 3% Pachyphyllum stem extract, add water and spices to moisten, put into a jar, sterilize at 0.1MPa for 30-45min, add liquid seeds after cooling and mix well. The fermentation temperature was 26°C, and after 15 days of fermentation, the contents of hypocrellin A, hypocrellin B, hypocrellin C, and hypocrellin D in the fermentation medium were 1351.2mg / 100g, 2006.7mg / 100g, respectively , 9.2mg / 100g, 5.7mg / 100g.

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Abstract

The invention belongs to the technical field of microbial fermentation, and in particular relates to a bamboo yellow fungus with high hypocretin production and its cultivation method and application. The bamboo yellow fungus of described hyperhypocretin is classified as bamboo yellow strain ( Shiraia Bambusicola ), has been preserved in China General Microorganism Culture Collection Center, and the preservation number is CGMCC No.18808. Hypocretin of the present invention can not only be fermented to obtain high-yield hypocrellin, the total amount of hypocrellin can reach 4762.9mg / 100g, and the obtained hypocrellin also includes hypocrellin A, hypocrellin B, Hypocretin C, Hypocretin D.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a bamboo yellow fungus with high hypocretin production and its cultivation method and application. Background technique [0002] Bamboo yellow (Shiraiabambusicola Henn), also known as bamboo flower, bamboo cocoon, bamboo red spot fungus, etc., belongs to Ascomycotina, Sclerotinia, Hypocreales, Hypocreataceae, and Bambusicola genus fungi. As a traditional parasitic medicinal fungus in my country, Phyllostachys praecox grows mostly in decaying or about to decay bamboo forests, and its main hosts are the bamboo plants of the genus Fargesia sp. and Brachystachyum sp. Folks use bamboo yellow soaked in wine to treat rheumatoid arthritis, sciatica, lumbar muscle strain, bruises, stomach pain due to deficiency and cold, convulsions in children, and acute hepatitis. Modern medical research shows that the medicinal value of bamboo yellow is mainly reflected in a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P15/00C12R1/645
CPCC12N1/14C12P15/00C12N1/145C12R2001/645
Inventor 袁建国孙博通高先岭
Owner JINAN GUOLI BIOLOGICAL SCI & TECH
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