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Shiraia bambusicola with high yield of Hypocrellin A and application of Shiraia bambusicola

A technology of Bamboo Rhododendron A and bacterial strains, applied in the field of bioengineering, can solve problems such as comprehensive utilization of secondary pollution of glycerol, and achieve the effects of environmental protection and high efficiency of comprehensive utilization, cost reduction and high conversion rate.

Active Publication Date: 2019-08-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the production of biodiesel increases year by year, a large amount of by-products of glycerin will be produced every year, and these glycerols have secondary pollution and comprehensive utilization problems

Method used

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  • Shiraia bambusicola with high yield of Hypocrellin A and application of Shiraia bambusicola
  • Shiraia bambusicola with high yield of Hypocrellin A and application of Shiraia bambusicola
  • Shiraia bambusicola with high yield of Hypocrellin A and application of Shiraia bambusicola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A wild-type strain isolated from the fruiting body of Zhuhuang from Zhejiang, China.

[0055] The bamboo yellow strain is inserted into the potato dextrose agar medium (PDA) for cultivation.

[0056] Mycelium morphology: At the initial stage of mycelium culture, the colonies are white, sparse, loose, and fluffy; after 6 days of culture, the pigment begins to secrete to the outside of the cell, and the back of the plate begins to appear yellowish brown; The pigments secreted by mycelia in contact with the base phase were distributed in concentric brown circles. The morphological characteristics of the hyphae are: the diameter of the aerial hyphae is 23-50 μm, with lock joints, and no conidia are produced. Wherein, the appearance colony form of bacterial strain when growing on PDA plate for 120h is as follows: figure 1 shown.

[0057] Physiological and biochemical characteristics of the strain: glucose, sucrose, lactose, glycerol, mannitol, galactose, soluble starch, c...

Embodiment 2

[0066] Take the strains cultured on the PDA plate for 5 days, punch holes and insert them into a Erlenmeyer flask containing 50ml of PDB medium (potato dextrose medium), place them in a shaker controlled at 150rpm, and cultivate them at 28°C for 48h to prepare a seed solution.

[0067] Take 2ml of seed liquid and insert into 50mL fermentation medium (containing 0.9g of maltose, 0.05g of beef extract, MgCl 2 0.001g) in an Erlenmeyer flask, placed in a shaker, at a temperature of 28°C and a rotation speed of 150rpm, the pigment fermentation culture was carried out for 72h.

[0068] After the shake flask culture was completed, the mycelium was centrifuged and washed with ultrapure water, and then vacuum freeze-dried for 12 hours. Dichloromethane was added according to the ratio of 1 g of mycelium to 30 mL, and ultrasonic extraction was carried out at 30 ° C for 30 min, and the ultrasonic power was 300 W. Extracts were separated and identified by HPLC. Chromatographic conditions...

Embodiment 3

[0071] Take the strains cultured on the PDA plate for 3 days, punch holes and insert them into a Erlenmeyer flask containing 50ml of potato dextrose agar medium PDB medium, place them in a shaker at 150rpm, and cultivate them at 28°C for 48h to prepare a seed solution.

[0072] Take 2ml of seed solution and put it into a conical flask containing 50mL of fermentation medium (containing 1.0g of glucose, 0.5g of peptone, and 0.001g of MgCl2), place it in a shaker, and carry out pigment fermentation at a temperature of 28°C and a speed of 150rpm for 72 hours. .

[0073] After the shake flask culture was completed, the mycelium was centrifuged and washed with ultrapure water, and then vacuum freeze-dried for 12 hours. Dichloromethane was added according to the ratio of 1 g of mycelium to 30 mL, and ultrasonic extraction was carried out at 30 ° C for 30 min, and the ultrasonic power was 300 W. Extracts were separated and identified by HPLC. Chromatographic conditions: chromatograp...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a strain of Shiraia bambusicola MH-02 with high yield of Hypocrellin A. The classified nomenclature of the strain is Shiraia bambusicola, and the preservation number is CDMCC No.60438. The strain is used for converting glycerinum into the high value-added Hypocrellin A, the conversion rate is high, and an environment-friendly and efficient pathway is provided for comprehensive utilization of the glycerinum.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bamboo yellow strain with high hypocretin A production and application thereof. Background technique [0002] Bamboo yellow, also known as bamboo flower, bamboo red dumpling, bamboo notoginseng, etc., has the functions of relieving cough and eliminating phlegm, dissipating blood stasis and dredging menstrual flow, promoting blood circulation and relieving pain, restoring tissue function, strengthening physical fitness, nourishing the middle and nourishing qi and calming down. It is a rare medicine in my country. fungal herbal medicine. Bamboo yellow is a sub-seat of Shiraia bambusicola. Bamboo yellow fungus belongs to the genus Phalaenopsis of the family Hypocrenaceae, and it specifically parasitizes on the shoots of the genus Brachycarpa species, and its sporophytes are often found in decaying bamboo forests. [0003] The core medicinal ingredients in Hypocrellin are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P5/00C12R1/645
CPCC12P5/005C12N1/145C12R2001/645
Inventor 田霄飞胡梦花张媛
Owner SOUTH CHINA UNIV OF TECH
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