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Method for improving hypocrellin yield in shiraia bambusicola

A kind of technology of rhodopsin and rhododendron, applied in directions such as fungi, fermentation, etc., can solve the problems such as increasing the output of rhodopsin, and achieve the effect of increasing the output

Active Publication Date: 2012-05-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the use of product in situ transfer technology to increase the production of hypocrellin and facilitate the separation of products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Hypocretin produced by bamboo yellow fungus fermentation

[0032]1. Activation of strains: take out the preserved bamboo yellow bacteria strain S8 and insert it into the newly prepared PDA medium. The composition of the PDA medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 potatoes (boiled juice) , glucose 20, agar 15, the pH value is the natural pH, the culture temperature is 25-28°C, and the culture time is 9-10 days;

[0033] 2. Insert the activated bacterial classification into a triangular flask equipped with a seed medium after being punched with a puncher. The composition of the seed medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 grams of potatoes (boiled juice), glucose 20, KH 2 PO 4 3. MgSO 4 1.5, vitamin B 1 0.01, yeast extract 5, the liquid volume is 200mL seed medium per 500mL Erlenmeyer flask, the inoculation volume is 4 holes / 200mL cultur...

Embodiment 2

[0041] 1. Activation of strains: take out the preserved bamboo yellow bacteria strain S8 and insert it into the newly prepared PDA medium. The composition of the PDA medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 potatoes (boiled juice) , glucose 20, agar 15, the pH value is the natural pH, the culture temperature is 25-28°C, and the culture time is 9-10 days;

[0042] 2. Insert the activated bacterial classification into a triangular flask equipped with a seed medium after being punched with a puncher. The composition of the seed medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 grams of potatoes (boiled juice), glucose 20, KH 2 PO 4 3. MgSO 4 1.5, vitamin B 1 0.01, yeast extract 5, the liquid volume is 200mL seed medium per 500mL Erlenmeyer flask, the inoculation volume is 4 holes / 200mL culture medium, the culture temperature is 25-28°C, the shaker speed is 150-160rpm, an...

Embodiment 3

[0051] 1. Activation of strains: take out the preserved bamboo yellow bacteria strain S8 and insert it into the newly prepared PDA medium. The composition of the PDA medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 potatoes (boiled juice) , glucose 20, agar 15, the pH value is the natural pH, the culture temperature is 25-28°C, and the culture time is 9-10 days;

[0052] 2. Insert the activated bacterial classification into a triangular flask equipped with a seed medium after being punched with a puncher. The composition of the seed medium is that each liter of distilled water contains the following ingredients (unit: gram): 200 grams of potatoes (boiled juice), glucose 20, KH 2 PO 4 3. MgSO 4 1.5, vitamin B 1 0.01, yeast extract 5, the liquid volume is 200mL seed medium per 500mL Erlenmeyer flask, the inoculation volume is 4 holes / 200mL culture medium, the culture temperature is 25-28°C, the shaker speed is 150-160rpm, an...

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Abstract

The invention discloses a technology for improving hypocrellin yield in shiraia bambusicola by using a polystyrene type nonpolar adsorption resin, wherein the technology comprises: carrying out activating culture for a strain, carrying out liquid seed culture, and carrying out co-culture fermentation for the strain and the adsorption resin. The method is characterized in that: with the co-culture fermentation, the resin can absorb the hypocrellin secreted by the shiraia bambusicola, and the hypocrellin content in the shiraia bambusicola can be improved so as to improve the hypocrellin yield. Therefore, with the present invention, the synthesis of the hypocrellin in the shiraia bambusicola can be effectively stimulated, the hypocrellin production in the culture system can be improved, the yield is high, the cost is low, the steps are simple, and the method can be used for the industrial production, and has a good industrial application prospect.

Description

technical field [0001] The invention belongs to microbial fermentation engineering, and in particular relates to a method for improving the output of hypocrellin in bamboo yellow bacteria by using a two-phase culture technology. Background technique [0002] Bamboo yellow fungus (Shiraia bambusicola P.Hennigs), also known as bamboo notoginseng, bamboo cocoon, bamboo red dumpling, etc., is a fungus that specifically parasitizes on some bamboo twigs. Hypocreaceae (Hypocreaceae) Bamboo yellow genus (Shiraia). Bamboo yellow contains a variety of physiologically active ingredients, the most eye-catching is hypocrellin (Hypocrellin). [0003] Hypocrellin belongs to the perylene quinone compound, which mainly contains two components: Hypocrellin A (Hypocrellin A, referred to as HA) and Hypocrellin B (Hypocrellin B, referred to as HB), of which more than 95% are A, HA It can be converted into HB by dehydration. Hypocretin is a new type of photosensitizer first discovered in my co...

Claims

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Application Information

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IPC IPC(8): C12P7/26C12N1/14
Inventor 王剑文赵一璐潘魏松雷晓珩陈可嘉
Owner SUZHOU UNIV
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