113 results about "Schizophyllum commune" patented technology

Disclosed herein is a method for high-yield production of Schizophyllum commune-derived beta-glucan having a homogeneous composition, comprising subjecting mycelia of Schizophyllum commune to liquid culture with an addition of a synthetic adsorbent, and a composition for external application comprising the beta-glucan produced therefrom, which is capable of relieving dry skin conditions, atopic diseases and itching.

Disclosed herein is a process for liquid cultivating Schizophyllum commune Fr. for isolation of beta-1,6-branched-beta-1,3-glucan and a composition for external application containing beta-1,6-branched-beta-1,3-glucan as an active ingredient, which can defer skin aging, impart skin whitening effect and cure skin damage effectively.

The invention discloses a production method of beta-glucan. The production method comprises the following steps of (1) schizophyllum commune activation; (2) seed activation; (3) fermentation culture; (4) coarse extraction; (5) purification. A colorless liquid culture medium prepared from 25 to 30g / L of glucose, 10 to 15g / L of sorbitol, 0.2 to 0.8g / L of monopotassium phosphate, 3 to 5g / L of epsom salt, 0.5 to 1g / L of ammonium sulphate, 0.1 to 0.5g / L of triethylamine hydrochloride, 1 to 2.2ml / L of trace element mixed solution and water is used as a fermentation culture medium for performing deep layer liquid fermentation culture on strains to obtain the colorless fermentation liquid. No pigment exists in the whole process; a decoloring step is not needed; loss due to adsorption is reduced; the problem of beta-glucan separation purification is overcome; high-purity white beta-glucan is obtained; the production method is suitable for mass industrial production.

The artificially cultivation and propagation technology of Schizophyllum commune Fr. includes tissue culture to purify and obtain wild strain of Schizophyllum commune Fr., artificially cultivation in combined culture material, domesticating, propagation, tissue separation and other steps. During the cultivation, the temperature, humidity, air flow and scattered sunlight are artificially controlled so as to produce large, high quality and high yield Schizophyllum commune Fr.

The invention discloses a lobster feed capable of enhancing the immunity. The feed is prepared from the following raw materials in parts by weight: 15 to 25 parts of germinated brown rice, 20 to 30 parts of oat bran, 45 to 85 parts of potato powder, 4 to 10 parts of carrot top, 8 to 16 parts of duck oil, 30 to 50 parts of chrysanthemum meal, 5 to 10 parts of peanut meal, 3 to 6 parts of earthworm powder, 8 to 12 parts of sunflower seed meal, 4 to 10 parts of extruded soya bean meal, 3 to 9 parts of meat and bone meal, 6 to 12 parts of defatted fish meal, 6 to 10 parts of concentrated albumen-powder of soybeans, 10 to 20 parts of potato protein powder, 0.06 to 0.12 part of tryptophan, 0.3 to 0.7 part of methionine, 0.5 to 0.7 part of cellulase, 1.5 to 2.5 parts of selenium-enriched yeast, 0.1 to 0.2 part of Bacillus, 0.06 to 0.1 part of monascus, 0.03 to 0.07 part of Schizophyllum commune, 4 to 8 parts of Chinese herbal medicine additive, 3 to 5 parts of corn oil and 3 to 6 parts of modified montmorillonite.

The invention belongs to the technical field of biological fermentation, and particularly relates to an active fermentation object, a preparation method of the active fermentation object, and application of the active fermentation object to cosmetics. The preparation method comprises the steps: S1 oat bran and water are taken to be mixed, then enzyme treatment is conducted, after enzyme treatment,sterilization is conducted, and an oat bran culture solution is prepared; S2 the oat bran culture solution is taken to be transferred into a fermentation tank, schizophyllum commune strains subjectedto three-stage culture are inoculated for fermentation, and a schizophyllum commune polysaccharide fermentation solution is obtained; and S3 the schizophyllum commune polysaccharide fermentation solution is taken to be filtered through a plate-and-frame filter press, a schizophyllum commune fermentation extracellular product is obtained, and then the schizophyllum commune fermentation extracellular product is micro-filtered and washed with water through a ceramic membrane till the clear and bright active fermentation object is obtained. The method is low in production cost, and simple and stable in process, and the obtained fermentation object containing active components such as oat beta-glucan and schizophyllum commune polysaccharide has the remarkable repairing and moisturizing effectson the skin, is high in stability, and has good application prospects in the field of the cosmetics.

The invention discloses a high-efficient cultivation method of schizophyllum commune. The method comprises the following steps of: inoculating a cultivar cultivated by domesticating wild schizophyllum commune into a cultivar culture medium which is prepared by components such as barley seeds or buckwheat seeds, broad leaf saw dust and the like and is subjected to high temperature and high pressure treatment; directly sheltering, shelving and arranging; exhausting air through a pricked hole; unsealing; cutting, and the like. Mushroom fruiting is managed at a proper temperature and high humidity; the grown schizophyllum commune has high quality; and the method is high in economic benefit, is easy to operate and practicable, and is an ideal schizophyllum commune manual planting technology.

The invention discloses an agarwood promoter and method for producing agarwood in Aquilaria sinensis. It is characterized in that the agarwood promoter is composed of following components in volume percentage: 5-10% of liquid cultivating Schizophyllum commune, 1.5-2% glucose or granulated sugar, 0.5-1% of acetate or malic acid, 85-95% of water. The method comprises the following steps: perforating Aquilaria sinensis trunk at a place not higher than 40cm through the bark, cambium, then perforating the secondary xylem, but hot hurting the primary xylem; introducing the agarwood promoter into the hole using a conduit and agarwood can be formed in half a year. The agarwood promoter of the invention has simple components and is easily acquired and prepared; the operation of the agarwood promoter to promote agarwood production is simple; the method of the invention has many advantages: obvious effect for effectively increasing agarwood productivity, shortening agarwood production period, better utilization and protection for Aquilaria sinensis resources.

The invention relates to biologic polysaccharide having effects of promoting hair generation, developing hair and preventing hair loss and an application of the biologic polysaccharide. Particularly,beta-glucan, particularly beta-glucan having natural solubility, high molecular weight and high viscosity (such as schizophyllum commune beta-glucan) can effectively promote hair development and / or prevent hair loss. Experiment indicates that the beta-glucan can obviously promote hair follicle in interruptio to enter the growth period, so that the hair follicle can be in the growth period for a long time, hair can be promoted to be regenerate and grow, the hair loss can be prevented, the hair can be developed, and the biologic polysaccharide has the efficacy of removing and preventing dandruffand relieving itching.

The invention discloses an efficient nutrient supplementation feed for dairy cattle, belongs to the field of feed additives, and in particular relates to a feed additive product for the dairy cattle. The efficient nutrient supplementation feed for the dairy cattle is prepared by adopting the following steps of: crushing calcium carbonate components in a slow release preparation, and mixing the crushed components with alpha-hydroxyl-4-methylthiobutyric acid evenly; mixing the mixture with other components in the slow release preparation evenly; crushing urea, soybean peptide powder, schizophyllum commune and lucid ganoderma to obtain materials with length or grain diameter of being less than 3 mm; mixing the preprocessed mixture with the crushed urea, soybean peptide powder, schizophyllum commune and lucid ganoderma; extruding the mixture by using a feed extruding unit; and mixing the extruded materials with extruded soybean. According to the efficient nutrient supplementation feed for the dairy cattle in the invention, the added edible-medical edible mushroom components not only have nutrient substances such as proteins, polysaccharide and the like, but also have a good lactation promotion effect; and the milk yield of the dairy cattle is effectively increased after the dairy cattle eat the efficient nutrient supplementation feed for the dairy cattle.

The invention discloses a method for regulating the molecular weight of schizophyllan as a fermentation product of schizophyllum commune franch. The method comprises the following steps: taking slopestrains of schizophyllum commune franch, and carrying out slope inoculation and culture by adopting a PDA culture medium test tube, thereby preparing fermentation seed liquid; inoculating the seed culture liquid into a fermentation tank for fermentation, wherein the initial stage of fermentation is 0-36 hours, and the fermentation conditions are controlled as follows: the stirring rotation speed is 100-400r / min, the ventilation volume is 0.20-0.8vvm, and the temperature is 26-28 DEG C; the middle stage of fermentation is 37-84 hours, and the later stage of fermentation is 85-96 hours; and respectively controlling the fermentation conditions, discharging after the fermentation is ended, and removing mycelia; concentrating, precipitating, filtering to obtain refined schizophyllan. Accordingto the method disclosed by the invention, polysaccharides are degraded by utilizing enzyme self-secreted by the thalli, so that not only is the procedure of degrading after separating and purifying crude polysaccharides with larger molecular weight and poorer solubility and obtained by fermentation in the traditional method greatly simplified, but also the product yield is increased and the production cost is reduced.

The invention discloses a Schizophyllum commune Fr high-yield fruiting body strain and a three-dimensional ecology-returning cultivation method, which comprises the following steps of: 1. strain collection and domestication; 2. strain screening; 3. optimum cultivation season; 4. bacteria package production; 5. inoculation; 6. bacteria package culture; 7. maturation culture; and 8. three-dimensional ecology-returning cultivation.

A method for reducing the toxicity of strychnos nux-vomica seeds by solid fermentation with medicinal fungi comprises the following steps of: activating and culturing schizophyllum commune, shiraia bambusicola, coriolus unicolor, cantharellus cibarius, beauveria bassiana and cordyceps cicadae for fermentation; oven-drying strychnos nux-vomica seeds, pulverizing, soaking in water, and autoclaving; respectively inoculating the above fungi on the treated strychnos nux-vomica seeds, performing solid fermentation under certain conditions, collecting respective fermentation product, and drying at low temperature to obtain medicinal fungal substances. The inventive method is simple and reasonable, and can obtain six medicinal fungal substances with reduced toxicity of strychnos nux-vomica seeds suitable for treatment purpose. The medicinal fungal substances have toxicity remarkably lower than that of strychnos nux-vomica seeds, have definite analgesic and antiphlogistic effects, and can keep the original main drug effect of Strychnos nux-vomica seed.

The invention discloses a schizophyllum commune strain for generating a volatile bacteriostatic and nematocidal active component and application thereof, and belongs to the field of separation and application of the schizophyllum commune strain. The schizophyllum commune strain for generating the volatile bacteriostatic and nematocidal active component is separated from poplar leaves; the microbial preservation number of the strain is CGMCC No.12772. A GC-MS (Gas Chromatography-Mass Spectrometer) analysis demonstrates that volatile gas generated by the strain includes 16 effective components, wherein beta-bisabolol is the main component. Bacteriostatic and nematocidal test results show that the schizophyllum commune strain separated by the invention can effectively prevent and control various bacterial or fungal diseases which are harmful to plant growth, and the generated volatile product has remarkable inhibitory effects on worms and spawns of nematodes. The invention further discloses a biological fumigant prepared by using the schizophyllum commune strain and application thereof for preventing and controlling various diseases or parastic nematodes which are harmful to plant growth.

The invention provides a macro fungus, namely white ginseng, derived from coastal mudflat; the macro marine fungus is identified to be schizophyllum commune which is capable of growing in a high-salinity culture medium and is capable of generating schizophyllan. The marine schizophyllum commune disclosed by the invention is screened by virtue of a natural seawater prepared restrictive medium by taking rotten woods from coastal and seaside wetlands as samples; and the schizophyllum commune is subjected to liquid fermentation, so that the marine schizophyllan is obtained. The chemical component of the schizophyllan produced by the invention is beta-1,3 / 1,6-glucan, which is derived from the marine schizophyllum commune and is applicable to the fields of food, cosmetics and medicines; and the schizophyllan is suitable for industrial production and is quite high in application and popularization values.

The invention discloses a production method of a fungal fermentation feed. The method comprises slant strain activation culture, liquid culture, liquid secondary seed culture and solid fermentation culture. The method provided by the invention is characterized in that the Schizophyllum commune is cultured at the temperature of 20-27 DEG C in the solid fermentation phase; after the Schizophyllum commune is cultured for 5-8 days, the temperature is reduced to 6-10 DEG C; after the Schizophyllum commune is cultured for 1-3 days, the temperature is increased to 20-27 DEG C; and the total culture time is 15-40 days. The product obtained by the method has stable performance, can be used safely, and can be matched with other feed without taboos. The additive amount of the product provided by theinvention in the feed is 20-100%.After feeding, the immunity and milking capacity of the animal organism can be obviously enhanced, and the daily gain and the feed conversion rate can be improved. The method provided by the invention meets the green production requirements of animal foods and has the advantages of obvious social benefit and economic benefit.

The invention discloses a feed for enhancing the immunity of lobsters. The feed is prepared from the following raw materials in parts by weight: 40-80 parts of corns, 15-25 parts of oat bran, 10-20 parts of radish leaves, 2-10 parts of fish oil, 2-8 parts of waxgourd peel, 5-15 parts of soybean protein, 1-8 parts of puffed meat and bone meal, 2-6 parts of potato protein powder, 2-6 parts of earthworm powder, 20-40 parts of sunflower seed meal, 2-8 parts of yeast protein powder, 1-4 parts of yellow mealworm, 1-6 parts of peanut meal, 5-10 parts of rice bran meal, 1-4 parts of modified montmorillonoid, 12 parts of corn oil, 1-2 parts of selenium-enriched yeast, 0.1-0.4 part of phytase, 0.01-0.06 part of Schizophyllum commune, 0.04-0.08 part of Armillaria mellea, 0.02-0.05 part of monascus, 0.04-0.08 part of lysine, 0.1-0.4 part of methionine and 1-4 parts of a Chinese herbal medicine additive.

The invention discloses a Schizophyllum commune protein extract and a preparation method and application thereof. The preparation method comprises the steps of leaching Schizophyllum commune mycelium powder in a phosphate buffer, and centrifuging to obtain a crude Schizophyllum commune mycelium extractive solution; and adding ammonium sulfate to the crude Schizophyllum commune mycelium extractive solution, standing for 6-12 h, and centrifuging to obtain the Schizophyllum commune protein extract. The invention also discloses a culture method of Schizophyllum commune and a fermentation culture medium for Schizophyllum commune. The Schizophyllum commune protein extract has good prevention and control effects on tobacco mosaic virus (TMV), and inhibition rate to TMV is over 70%. The Schizophyllum commune protein extract belongs to natural extracts, is harmless to human and environment, and belongs to environment-friendly pesticides. The preparation method has the advantages of simple process, low cost, and applicability to large-scale production. In addition, the yield of Schizophyllum commune is high by using the fermentation culture medium.

Disclosed herein is a process for liquid cultivating Schizophyllum commune Fr. for isolation of beta-1,6-branched-beta-1,3-glucan and a composition for external application containing beta-1,6-branched-beta-1,3-glucan as an active ingredient, which can defer skin aging, impart skin whitening effect and cure skin damage effectively.

The invention provides a nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, essence lotion, a preparation method of the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, and an application of the essence lotion. The nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin comprises the symbiotic bacterium combined fermented products, decarboxy carnosine HCl, palmitoyl pentapeptide-4, tocopheiyl acetate, a pale butterflybush flower extract, resveratrol, a pomegranate fruit extract and a narcissus bulb extract, wherein symbiotic bacteria of the symbiotic bacterium combined fermented productscomprise combination of schizophyllum commune, thermus thermophilus, microzymes and lactobacilli, and fermentation raw materials of the symbiotic bacterium combined fermented products comprise combination of rice, coix lacryma-jobi ma-yuen seeds, ginseng, kelp and raspberries. Through synergistic compounding of effective active components of the symbiotic bacterium combined fermented products andthe like, the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin can condition and repair skin ageing resistant routes of oxidation resistance, saccharification resistance, blue light resistance, collagen synthesis promotion and the like, so that cell vitality is protected, and the skin has the capacity for resisting exogenous environmental factors.

The invention provides a culture medium for cultivation of schizophyllum commune fr. The culture medium is composed of the following components by the mass percentage: 70-90% of radix astragali residues, 1% of a gypsum powder, 1% of calcium superphosphate, and 8%-28% of wheat bran, wherein the sum of the percentages of the components is 100%. The radix astragali residues are used as the main basematerial and are used for cultivating fruiting bodies of the schizophyllum commune fr., fruiting can be successfully realized, and the fruiting bodies of the schizophyllum commune fr. are harvested; the fruiting bodies of the schizophyllum commune fr. are valuable food-medicine fungi and have high economic value. The waste radix astragali residues can be reused.

The invention discloses a method for preparing rare ginsenoside by using schizophyllum commune for biotransformation of ginsenoside. A novel method is used for biotransformation of ginsenoside, duringa fermentation process, schizophyllum commune is capable of degrading ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan, polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. For biotransformation ofginsenoside, during the fermentation process, schizophyllum commune can degrade ginsenoside to generate a lot of rare saponins, a lot of various biologically-active substances such as schizophyllan,polypeptide, alkaloid, enzyme, amino acid, and vitamin can be simultaneously generated; and health care and medicinal efficacy of a fermentation product can be increased with a maximum. The method canbe used for preparing a monomer rare saponin, and can be directly extract a crude extract product for product exploitation, a simple processing purpose with high efficiency, safety, environment protection, and low cost can be achieved, and the method provides guarantee for industrial production, and food and new drugs preparation.

The invention discloses a ginseng endophytic fungus. The ginseng endophytic fungus is separated from a living plant body of Araliaceae Panax species Panax ginseng C.A. Mey. with an endophytic fungus separation and purification technology and is identified as Schizophyllum commune according to microbial taxonomy. The strain is collected in the CGMCC (China General Microbiological Culture Collection Center) (Institute of Microbiology, Chinese Academy of Sciences), and the collection number is CGMCC 11009. The invention further discloses an application of the ginseng endophytic fungus to increase of the biomass of tissue culture ginseng hairy roots of host ginseng and the content of ginsenosides in the host ginseng.

The invention relates to a method for utilizing schizophyllum commune Fr. mycelia for preparing a degradable packaging material. The method comprises the steps of S101, preparation of a culture material, wherein corn straw, wheat straw, apple wood dust, wheat hulls, cottonseed hulls, lime and gypsum are uniformly mixed; water is added into the culture material for uniform mixing and sterilizing; S102, preliminary culturing of a culture, wherein a schizophyllum commune Fr. liquid culture is inoculated to a fungus bag which is cooled to the room temperature, and the fungus bag is covered with alid for culturing; S103, mold filling and culturing, wherein a disinfected packaging mold is filled with the material which is grown with the schizophyllum commune Fr. mycelia under aseptic conditions, and continuous culturing is conducted after compacting and sealing are conducted; S104, sample drying, wherein after the schizophyllum commune Fr. mycelia in the material of the mold are reunited, samples are taken out of the mold and dried to obtain the product. According to the method, the environment-friendly packaging material is prepared with the crop straw as main raw materials, the applewood dust and the wheat hulls as auxiliary raw materials and the schizophyllum commune Fr. mycelia as the binder, the problem that existing packages are non-degradable can be solved, and crop straw and fruit tree branches can also be better treated.

The invention discloses a nutrient feed suitable for all-age pigs. The nutrient feed is prepared from the following raw materials in parts by weight: 20-55 parts of corn, 20-38 parts of rye, 20-40 parts of rice bran, 1-4 parts of lard oil, 2-7 parts of chrysanthemum cake, 5-10 parts of corn bran, 2-7 parts of feather meal, 10-20 parts of corn germ meal, 8-14 parts of sunflower cake, 2-6 parts of silkworm powder, 5-10 parts of seaweed meal, 2-10 parts of echinopsis tubiflora powder, 1-3 parts of alfalfa, 2-4 parts of aloe powder, 2-5 parts of zeolite powder, 1-3 parts of sodium selenate, 1-2 parts of cerium citrate, 1-2 parts of ferrous glycine chelate, 2-10 parts of a Chinese herbal medicine additive, 0.2-0.6 part of protease, 0.2-0.45 part of amylase, 0.2-0.6 part of pectinase, 0.2-0.9 part of phytase, 0.1-0.3 part of schizophyllum commune, 0.1-0.4 part of armillaria mellea and 2-5 parts of a phagostimulant. The nutrient feed is suitable for all-age pigs, green, healthy and balanced in nutrient.

The invention discloses a method for degrading and modifying schizophyllan by using self-produced endonuclease of a schizophyllum commune Fr. fermentation system. The method comprises the steps of firstly, carrying out revaccination, activation and culture on a slant strain of schizophyllum commune Fr. by using a PDA culture medium to obtain fermented seed liquid; inoculating in the fermented seedliquid, continuously culturing and fermenting under the conditions that the stirring speed is 100-250rpm, the ventilation rate is 0.2-0.7vvm and the culture temperature is 26-29 DEG C; then, carryingout ceramic microfiltration membrane separation, modifying polysaccharide in filtrate by means of enzymolysis, and separating the modified polysaccharide to obtain the modified polysaccharide. Afterthe method is adopted, the molecular weight of the modified polysaccharide is reduced to 1500kDa or less from 4000kDa or more by means of degradation, and the modified polysaccharide can be completelydissolved in distilled water within 24h. The method provided by the invention utilizes enzyme secreted by the strain to degrade the polysaccharide, thus not only solving the problem that specific enzyme is difficult to obtain, but also greatly simplifying the technology and lowering the production cost; therefore, the method for obtaining the soluble polysaccharide has industrial potential.

The invention relates to a hair-growing and hair-consolidating caring product. In particular, a composition of the present invention comprises schizophyllum commune beta-glucan, a surfactant, and a cosmetically acceptable carrier. The composition of the invention not only enhances the hair cleansing and caring effect, but also enhances the hair hair-growing, hair loss-prevention, anti-dandruff andanti-itch effect, reduces the irritation to the scalp, thus achieving the excellent balance of cleansing care, hair growth and hair loss prevention, anti-dandruff and itching relieving, and scalp irritation reduction. In addition, the composition of the present invention also increases hair softness, improves dry and wet combability, and reduces hair tangling. The composition is especially for solving the disadvantages of dryness and difficult combing of the hair after using a silicone oil-free shampoo, and has the excellent improvement effect.

The invention discloses a method for extracting active polysaccharide of wheat bran. The method comprises seed liquid preparation, fermentation medium preparation, fermentation and active polysaccharide extraction and concretely comprises inoculating nutrient broth with revived schizophyllum commune, bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae according to a weight ratio,carrying out culture to obtain a seed liquid, mixing wheat bran, soybean meal, corn core powder, distilled water and a fermentation synergist to obtain a fermentation medium, inoculating a fermentation medium with the seed liquid, carrying out fermentation culture, and extracting the fermented wheat bran through distilled water and an ethanol solution to obtain an active polysaccharide solution. The fermentation synergist can greatly increase the yield of the active polysaccharide. The method can significantly reduce the probability that the wheat bran active polysaccharide is separated alongwith the protein denatured gel and increase the yield and content of the wheat bran active polysaccharide. The wheat bran active polysaccharide can be used as an antioxidant active ingredient to remove free radicals in the body.

The invention discloses a Schizophyllum commune efficient breeding-planting method, and belongs to the artificial edible fungus cultivation. The method comprises the following steps: inoculating a stock seed cultivated by use of wild Schizophyllum commune into a potato fluid culture medium to breed cultivar fungus ball fluid, propagating by use of a fluid strain cultivator, and then inoculating into a cultivation fungus bag by use of an inoculation gun, and directly planting in a greenhouse to cultivate the fungus. Therefore, the fungus bag inoculation efficiency is greatly improved, the cost is lowered since the wood flour tree species of the burdening in the fungus bag are unlimited; the Schizophyllum commune efficient breeding-planting method is a Schizophyllum commune cultivation technology which is good in economic benefit and practical.

The invention relates to the technical field of microorganisms, specifically to Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, a method for production of huperzine A from the same, and application. The invention provides a Phlegmariurus phlegmaria mingchegensis mycorrhizal fungus named as Schizophyllum commune MT39, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC M2013642. According to another technical scheme, the invention provides a Phlegmariurus phlegmaria mingchegensis mycorrhizal fungus named as Chaunopycnis alba MT78, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC M2013643. According to the invention, the problem of difficult separation and purification of endogenous mycorrhizal fungi due to a high bacterium content in the soil can be solved, and the mycorrhizal fungi for producing huperzine A can be separated from rhizosphere soil.