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Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, method for production of huperzine A from the same, and application

A technology of mycorrhizal fungi and huperzine A, applied in the field of microorganisms, can solve the problems of large workload of separation and purification, different types of microorganisms, etc., and achieve the effect of great application value.

Active Publication Date: 2014-06-04
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of the above indicate that it is possible to produce huperzine A by fermentation of endophytic fungi. According to the internal mechanism of the symbiont, the mycorrhizal fungi producing huperzine A can be isolated from the rhizosphere soil of plants, but the current research on this mechanism Research points of view are not the same and there are many kinds of microorganisms in the soil, and the workload of isolation and purification is heavy. Therefore, there have been no reports on the isolation of huperzine-producing mycorrhizal fungi from plant rhizosphere soil.

Method used

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  • Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, method for production of huperzine A from the same, and application
  • Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, method for production of huperzine A from the same, and application
  • Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, method for production of huperzine A from the same, and application

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Embodiment 1

[0034] 1. Observation of bacterial strain micromorphology: inoculate two kinds of huperzine A-producing mycorrhizal fungal joints of the present invention in the center of the flat plate, then insert the sterilized cover glass obliquely at 45 ° into the flat plate of the inoculated bacteria (2 Slices / plates) were cultivated in a fungal incubator at 28°C. After the strains had grown to a certain extent (6 days), inserts (in a clean bench) were taken and observed under a microscope.

[0035] see figure 1 It is a micromorphological diagram of Schizophyllum MT39 in the present invention, and the microscopic morphology of the Schizophyllum MT39 is: mycelium is cotton-like, has septa, branches, and does not produce spores.

[0036] see figure 2 It is a micromorphological diagram of MT78 of the present invention, and the microscopic morphology of MT78 is: branched hyphae without septa, pear-shaped spores, and single cells.

[0037] 2. Observation of strain plate morphology: the my...

Embodiment 2

[0053] One, the collection of the mycorrhizal fungus of Sargassum cuspidatum in Fujian and Zhejiang provinces of the present invention

[0054] 1. The mycorrhizal fungi were isolated and purified by the soil dilution separation method from the rhizosphere soil samples of Sargassum chinensis collected from Guanzhai Mountain, Liancheng County, Longyan City, Fujian Province. Weigh 0.5g of the rhizosphere soil of Chinese fir from Fujian and Zhejiang provinces collected at the sampling point, pour it into a triangular flask filled with 4.5ml of sterile water and glass beads, vibrate for 5min, let it stand for 20min, and filter the supernatant to obtain 10 -1 diluent. Take 4 test tubes filled with 4.5ml sterile water. Press 10 -2 -10 -5 No., use a sterilized 1ml pipette to draw 0.5mL10 -1 diluent to mark 10 -2 In the test tube, use a pipette gun to repeatedly blow and suck three times and mix well, which is 10 -2 diluent. legally made 10 -3 -10 -5 diluent. Use a sterile pi...

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Abstract

The invention relates to the technical field of microorganisms, specifically to Phlegmariurus phlegmaria mingchegensis mycorrhizal fungi, a method for production of huperzine A from the same, and application. The invention provides a Phlegmariurus phlegmaria mingchegensis mycorrhizal fungus named as Schizophyllum commune MT39, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC M2013642. According to another technical scheme, the invention provides a Phlegmariurus phlegmaria mingchegensis mycorrhizal fungus named as Chaunopycnis alba MT78, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC M2013643. According to the invention, the problem of difficult separation and purification of endogenous mycorrhizal fungi due to a high bacterium content in the soil can be solved, and the mycorrhizal fungi for producing huperzine A can be separated from rhizosphere soil.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a mycorrhizal fungus of Sargassum spruce from Fujian and Zhejiang and a method and application thereof for producing huperzine A. Background technique [0002] Alzheimer's disease is a serious, degenerative brain disease. Due to its high incidence and high disability rate, it has caused serious harm and huge economic burden to society and families. With the aging of society, the incidence of Alzheimer's disease According to statistics, there are more than 50 million elderly people in the world suffering from different degrees of Alzheimer's disease, so the treatment of Alzheimer's disease is a major problem in the current society. [0003] Huperzine A [(-) Huperzine A, Hup A] is a new effective monomer of lycopodium alkaloids isolated from Huperzine serrata in 1986 by Chinese scientist Liu Jiasen. Pharmacological experiments show that it can inhibit the activity of acetyl...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/12C12R1/645
Inventor 吴水生刘海元张方方郑雅媗
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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