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151results about How to "Efficient secretion" patented technology

Method and Apparatus for Electrical Stimulation of the Pancreatico-Biliary System

The present invention is directed to a method and apparatus for electrical stimulation of the pancreatico-biliary system. Electrode sets are placed in the pancreatico-biliary system in an arrangement that induce contractions or relaxation of the portion or whole of the pancreatico-biliary system by electrical stimulation of the surrounding tissue, muscles and nerves. The electrical stimulus is applied for periods of varying duration and varying frequency so as to produce the desired therapeutic effect, including inhibiting fat digestion or fat absorption by a patient and inducing satiety in the patient.
Owner:SHARMA VIRENDER K

Secretion signal vectors

InactiveUS7071172B2Minimize potential deleterious side effectAttenuate seizure activityBiocideAnimal repellantsHeterologousAdeno associate virus
The present invention provides delivery vectors for transferring a nucleic acid sequence to a cell in vitro, ex vivo or in vivo. The delivery vector comprises a segment encoding a secretory signal peptide. In embodiments of the invention, the delivery vector is an adeno-associated virus (AAV) vector. In other embodiments, the secretory signal peptide is a fibronectin secretory signal peptide (including variations and modifications, thereof). The delivery vectors of the invention may further comprise a heterologous nucleic acid sequence encoding a polypeptide of interest for transfer to a target cell, where the polypeptide of interest is operably associated with the secretory signal. Also disclosed are methods of transferring a nucleic acid of interest to a cell using the delivery vectors of the invention.
Owner:THE UNIV OF NORTH CAROLINA AT CHAPEL HILL

Method for secretory production of human growth hormone

A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.
Owner:MITSUI CHEM INC

Recombinant engineering bacteria for efficiently expressing human growth hormone, construction method and application

The invention discloses recombinant engineering bacteria for efficiently expressing human growth hormone (hGH), a construction method and an application and provides an escherichia coli operon for expressing recombinant hGH, an expression plasmid containing an operon sequence and engineering bacteria QJSW-SZ01 (CGMCC No:7258) used for secretory expression of the recombinant hGH and obtained by transformation of the expression plasmid containing the operon. The recombinant engineering bacteria are characterized in that a coding sequence of a signal peptide of an escherichia coli heat-stable enterotoxin is changed, and rare codons of escherichia coli in the coding sequence are mutated so as to avoid formation of a secondary structure; meanwhile, an amino acid is altered on the terminal of the coding sequence so that the coding sequence is more beneficial to guidance of secretory expression of hGH; the signal peptide, an escherichia coli alkaline phosphatsae promoter (phoA promoter) and an escherichia coli T7 terminator are combined to be used as an expression control element so that the recombinant hGH is efficiently secretory-expressed in the escherichia coli in a soluble form. The recombinant engineering bacteria lay the foundation of finally developing a low-cost hGH pharmaceutical product.
Owner:吉林省奇健生物技术有限公司

Skin cream containing camellia seed oil and preparation method thereof

InactiveCN108309831AGeneral spreadMoisturizes and regulates oil secretionCosmetic preparationsToilet preparationsWaxChemistry
The invention discloses skin cream containing camellia seed oil. The skin cream containing camellia seed oil comprises 100 parts of water, 0.5 to 5 parts of p-hydroxyacetophenone, 1 to 8 parts of betaine, 0.1 to 0.8 parts of carbomer, 5 to 15 parts of glycerin, 5 to 15 parts of butanediol, 0.05 to 0.3 parts of sodium hyaluronate, 1 to 10 parts of nicotinamide, 0.05 to 0.5 parts of dipotassium glycyrrhizinate, 10 to 25 parts of camellia seed oil, 5 to 15 parts of mink oil, 1 to 5 parts of cetearyl alcohol and / or cocoyl glucoside, 2 to 10 parts of jojoba seed oil, 2 to 5 parts of stearate, 2 to10 parts of avocado fruit fat, 2 to 10 parts of cyclopentadimethylsiloxane, 2 to 10 parts of squalane, 2 to 10 parts of polydimethylsiloxane, 2 to 10 parts of isodecyl isononanoate, 3 to 10 parts of triethylhexanoin, 1 to 10 parts of tocopherol, 0.1 to 0.8 parts of triethanolamine, and 0.5 to 5 parts of 1, 2-hexanediol. The invention discloses a preparation method of the skin cream containing camellia seed oil. The preparation method utilizes scientific combination of the raw materials and realizes moisturizing, wrinkle resistance, aging resistance, sunscreen and skin repair. The product is refreshing and non-greasy and is suitable for different types of skin.
Owner:珠海美高美健康科技有限公司

High efficiency preparation method of high temperature alpha-amylase and mutant thereof

A high temperature alpha-amylase and a high efficiency preparation method of the mutant thereof belongs to the fermentation engineering field. The invention provides a method for high efficiently secreting expressing a high temperature alpha-amylase and mutant thereof with alpha-amylase activity through a bacillus, especially a bacillus licheniformis mutant strain as a host cell. The synthesis and secretion level of the high temperature alpha-amylase or mutant thereof of the recombinant strain of the invention reaches 18-25mg / mL during fed-batch fermentation and is 3-4.5 times of the original strain high temperature alpha-amylase synthesis level. The high temperature alpha-amylase of the invention is useful for reducing the fermentation manufacture cost of the industrial enzyme, simplifying the fermentation manufacture process and reducing fermentation industrial environment pressure.
Owner:福建福大百特生物科技有限公司

PelB signal peptide mutant capable of improving protein secretion efficiency and application of pelB signal peptide mutant

The invention discloses a pelB signal peptide mutant capable of improving the protein secretion efficiency and an application of the pelB signal peptide mutant and belongs to the technical field of gene engineering. A sequence of a pelB mutant secretive expression signal peptide is as shown in SEQ ID NO.1. The signal peptide is capable of improving the extracellular enzyme activity of objective protein cyclodextrin glycosyltransferase by 1.6 times. The extracellular protein production capability of recombinant escherichia coli transformed by using the signal peptide is strengthened, and industrialized production is facilitated.
Owner:JIANGNAN UNIV

Antibacterial maca spray

The invention discloses antibacterial maca spray. The antibacterial maca spray comprises, by weight, 0.5-1.0% of maca extract liquid, 1.0-1.5% of cinnamon extracts, 0.1-0.15% of clove extract liquid, 0.1-0.15% of capsicum annuum extract liquid, 0.1-0.5% of cistanche deserticola extracts, 0.3-0.5% of chlorhexidine acetate and the balance water. The antibacterial maca spray has the advantages that inhibition effects can be realized for hemolytic streptococcus, streptococcus pneumoniae, Escherichia coli bacteria, proteus vulgaris, salmonella paratyphi A, shigella dysenteriae, Escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, candida albicans, pseudomonas aeruginosa and the like, the antibacterial maca spray can be used for inhibiting the Escherichia coli, the staphylococcus aureus, the candida albicans and the like and has little skin irritation, and good wetting effects can be realized.
Owner:WEIFANG HONGYANG PHARMACY CO LTD

Photosynthetic production of 3-hydroxybutyrate from carbon dioxide

Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg / l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.
Owner:ARIZONA STATE UNIVERSITY

Construction and application of TRAIL (Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand) recombinant bacille calmette guerin (rBCG)

The invention provides construction of TRAIL (Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand) recombinant bacille calmette guerin (rBCG), and relates to a shuttle expression vector comprising a signal peptide fragment of a major secretory antigen Ag85B of BCG and a gene fragment of a TRAIL and a construction method thereof. The obtained shuttle expression vector pMV261-Ag85B-TRAIL is used for constructing rBCGTRAIL, and can be applied to preparation of TRAIL rBCG for treating superficial bladder tumors, preventing postoperative recurrence thereof and preventing tuberculosis. The rBCG has dual functions of TRAIL and BCG, so that cooperative and synergistic actions of the TRAIL and BCG can be better brought into play; rBCG-TRAIL can secrete TRAIL, and the using amount of the rBCG-TRAIL can be lower than that of the BCG under the condition that the same or better immune effect is achieved, so that the toxic or side effect is reduced; and the rBCG-TRAIL can directly secrete TRAIL efficiently on a certain part, so that tumor cells can be killed in cooperation with the rBCG-TRAIL, and high cost caused by the use of a foreign cell factor is avoided.
Owner:沈周俊
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