Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lignin biological resource utilization method

A biological resource and lignin technology, applied in the field of biochemical industry, can solve the problems of resource waste, environmental pollution, unreasonable utilization of resources, etc., and achieve the effects of improving enzyme activity, improving biodegradation rate and utilization efficiency

Active Publication Date: 2019-07-09
XI AN JIAOTONG UNIV
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current problems of low lignin degradation efficiency and unreasonable utilization of resources, such as environmental pollution and waste of resources, the invention provides a method for cultivating composite fungal flora to degrade and utilize lignin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lignin biological resource utilization method
  • Lignin biological resource utilization method
  • Lignin biological resource utilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Prepare medium 1 and 3 (the components are shown in Table 1), add the corresponding prepared medium solution into the bioreactor, supplement ultrapure water to a certain volume, and add 1g / L woody For plain straw, the filtered glucose solution was added after the culture medium was sterilized and cooled to room temperature.

[0053] Steam sterilize the prepared bioreactor (maintain at 121°C for 20 minutes). When the temperature drops to 80°C, take it out of the sterilizer and place it in a clean bench to cool. After cooling to room temperature, add the corresponding amount of glucose liquid.

[0054] 10% of the volume of the liquid was inserted into the seed liquid of the chaga to start continuous fermentation, and the fermentation process was maintained at 30°C, 200 rpm, pH 7.0 and ventilation 0.7vvm for continuous cultivation for 20 days.

[0055] During the fermentation process, continuous sampling was performed to determine Lac and MnP enzyme activities (see figur...

Embodiment 2

[0057] Prepare culture medium 2 and 3 (see Table 1 for its components), add the corresponding culture medium and trace element solution 2 (see Table 2) into the bioreactor, add ultrapure water to a certain volume, and 1g / L lignin straw was added to some experimental groups, and the filtered glucose solution was added after the culture medium was sterilized and cooled to room temperature.

[0058] Steam sterilize the prepared bioreactor (maintain at 121°C for 20 minutes). When the temperature drops to 80°C, take it out of the sterilizer and place it in a clean bench to cool. After cooling to room temperature, add the corresponding amount of glucose liquid.

[0059] Add Trametes versicolor seed solution according to the volume of 10% of the liquid to start continuous fermentation, keep 35°C during the fermentation process, and continuously cultivate for 20 days under the conditions of 100rpm, pH5.0 and ventilation 0.2vvm.

[0060] During the fermentation process, continuous sam...

Embodiment 3

[0062] Prepare culture medium 3 (see Table 1 for its components), add the corresponding culture medium solution and trace element solution 2 (see Table 2) into the bioreactor, supplement ultrapure water to a certain volume, and in some experiments 1g / L lignin wheat was added to the group, and the filtered glucose solution was added after the culture medium was sterilized and cooled to room temperature.

[0063] Steam sterilize the prepared bioreactor (maintain at 121°C for 20 minutes). When the temperature drops to 80°C, take it out of the sterilizer and place it in a clean bench to cool. After cooling to room temperature, add the corresponding amount of glucose liquid.

[0064] Insert 10% of the volume of the liquid into the mixed seed liquid of chaga and Trametes versicolor (volume ratio 1:1) to start continuous fermentation, and maintain the conditions of 25 ° C, 300 rpm, pH 4.0 and ventilation rate 0.5 vvm during the fermentation process continuous culture for 20 days.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lignin biological resource utilization method. According to the method, lignin can serve as a fermentation auxiliary substrate, continuous high-density culture is implemented, and the lignin can be degraded into substances such as acetic acid, vanillin, guaiacol, veratryl alcohol and phenolic compounds by fungal complex floras. The lignin can be applied to the fungal complex floras and mass efficient production of active substances of the fungal complex floras. Culture conditions and culture medium components of the fungal complex floras are optimized, the biodegradation rate and the conversion efficiency of the lignin are improved, so that the degradation rate of the lignin is not lower than 40%, and the concentration of a degradation product is 10-100mg / L. An efficient resource utilization method is provided for degradation and conversion of the lignin.

Description

technical field [0001] The invention relates to a method for realizing resource utilization of lignin through high-density cultivation of microbial compound flora, belonging to the field of biochemical industry. Background technique [0002] Due to the high development and modernization of human society, the demand for limited fossil energy is gradually increasing, and the burden on the environment is increasing. In order to achieve sustainable development and protect the environment, a sustainable renewable energy is needed Replace the fossil energy that the society is highly dependent on. [0003] Lignocellulose can be derived from plants such as rice straw, straw, and wheat. It is the most abundant renewable biomass on earth and has always been regarded as a substitute for the production of fuel oil and high value-added products. Lignocellulose is composed of cellulose, hemicellulose and lignin, among which cellulose has the highest content, followed by lignin, accountin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/40C12P7/24C12P7/22C12P7/02C12R1/645
CPCC12P7/02C12P7/22C12P7/24C12P7/40C12P39/00C12P2203/00
Inventor 费强崔堂武袁波傅容湛
Owner XI AN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com