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589 results about "Guaiacol" patented technology

Guaiacol (/ˈɡwaɪəkɒl/) is a naturally-occurring organic compound with the formula C₆H₄(OH)(OCH₃), first isolated by Otto Unverdorben in 1826. Although it is biosynthesized by a variety of organisms, this yellowish aromatic oil is usually derived from guaiacum or wood creosote. Samples darken upon exposure to air and light. Guaiacol is present in wood smoke, resulting from the pyrolysis of lignin. The compound contributes to the flavor of many substances such as whisky and roasted coffee.

Method for preparing aromatic compound by catalytic hydrocracking of lignin

The invention relates to a method for preparing an aromatic compound by direct catalytic cracking of lignin. Under the combined action of a catalyst and a solvent, industrial lignins such as natural lignin, sodium lignin sulfonate, alkali lignin and dealkalized lignin are used as raw materials and are selectively cracked into the aromatic compound derived from C6-C9 phenolic group, guaiacol group or syringyl through the catalytic process. The conversion rate of the raw materials reaches over 50%, and the guaiacol group and the syringyl aromatic compound account for over 90% in a cracking product. The method has the characteristics of cheap raw materials, high conversion rate, high yield of the aromatic compound and mild reaction condition.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for preparing aromatic hydrocarbon by carrying out catalytic hydrodeoxygenation on lignin

The invention relates to a method for preparing aromatic hydrocarbon by carrying out catalytic hydrodeoxygenation on lignin. A catalyst used in the method provided by the invention comprises two active components, namely an acid site being one or combination of more than one of transition metal elements niobium, tantalum, zirconium, molybdenum, tungsten and rhenium and a hydrogenation or hydrogen transfer active site being one or more than one of ruthenium, platinum, palladium, iridium, iron, cobalt, nickel and copper. According to the method provided by the invention, a phenol group, a guaiacol group, a syringa phenolic group compound, natural lignin and industrial lignin are taken as raw materials, water is taken as a solvent, high selectivity catalytic hydrodeoxygenation is carried out at the temperature of 180-350 DEG C and hydrogen pressure of 0.1-5MPa or with methyl alcohol, isopropyl alcohol and formic acid as hydrogen sources, so that C6-C9 aromatic hydrocarbon is obtained, the highest mass yield of aromatic hydrocarbon is 10%, and content of aromatic hydrocarbon in product oil can be up to more than 75%. The method provided by the invention has the advantages that reproducible natural biomass can be used as a raw material, and the raw material is cheap and available; the water is taken as the solvent, so that a reaction process is environment-friendly; and content of aromatic hydrocarbon in the product is high, and reaction conditions are mild.
Owner:EAST CHINA UNIV OF SCI & TECH

Microbiological process for producing vanillin

A microbiological process for producing vanillin and other useful products from ferulic acid is disclosed. Vanillin and guaiacol are recovered from the process using a differential pH extraction where the guaiacol is recovered at a pH of greater than 9 and the vanillin is recovered at a pH of from about 5 to about 8.
Owner:GIVAUDAN SA

Method for depolymerizing lignin into aromatic compounds under conditions of no additional hydrogen

The invention relates to a method for depolymerizing lignin into aromatic compounds under the conditions of no additional hydrogen. According to the method, biomass-base alcohol compound reformation hydrogen production reaction and lignin hydrodepolymerization reaction are combined; and in the reaction process, the biomass-base alcohol compounds are subjected to in-situ reformation hydrogen production, and meanwhile, the hydrogen is used for hydroepolymerizing lignin. The lignin is selectively cracked into C6-C9 aromatic compounds of phenolic group, guaiacol group, lilac group or other derivatives under the action of the catalyst. The conversion rate of the raw materials is up to higher than 50%, and the content of the guaiacol-group and lilac-group aromatic compounds in the cracked product can reach higher than 90%. The method uses the hydrogen prepared by biomass-base alcohol in-situ reformation as the hydrogen source, and avoids using high-pressure hydrogen in the preparation route; and thus, the method has the characteristics of high safety coefficient in the reaction process, high yield of the aromatic compounds, and mild reaction conditions.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Barbecue essence and production process thereof

InactiveCN101991084AEnhance characteristic flavorIncrease aromaFood preparationGuaiacolMethyl group
The invention discloses a barbecue essence, which is prepared from the following raw materials: ethyl maltol, 4-hydroxyl-2,5-dimethyl-3(2H)-furanone, 2,5-dimethyl-2,5-diyhydroxyl-1,4-dithiacyclo-hexane, methyl cyclopentenotone, 2-acetylpyrazine, 2-methyl tetrahydrofuran-3-mercaptan, bis(2-methyl-3-furyl) disulfide, propyl2-methyl-3-furyl disulfide, 4-methyl-5- hydroxyethyl-thiazole, 2-methylpyrazine, 2,3,5-trimethylpyrazine, 3-methylmercaptopropionaldehyde, difurfuryl disulfide, 4-methyl-4-furfurylthio-2-pentanone, 2,4,5-trimethylthiazole, 2,4,6-triisobutyl-1,3,5-dithiazine, beta-phenylethylmercaptan, 1,6-ethanthiol, 2-pentylthiophene, furfuryl mercaptan, 4,5-dimethyl-2-isobutyl-3-thiazoline, guaiacol, delta-dodecalactone, 2,4-decadienealdehyde, trans,trans-2,4- nonadienal, anisic aldehyde, butanoic acid, acetic acid, black pepper essential oil ginger essential oil, clove oil, cassia oil and an oil-soluble solvent. The invention also discloses a barbecue essence production process.
Owner:厦门市顶味兴业香料发展有限公司

Tobacco essence and preparation method thereof

The invention belongs to the technical field of tobacco additive, and relates to tobacco essence and a preparation method thereof. The tobacco essence comprises the following components in percentage by weight: 4.5 to 7.5 percent of tamarind extract, 1.5 to 4.5 percent of mimetic citronella extract, 0.5 to 1.5 percent of dried plum extract, 16.5 to 19.5 percent of liquid glucose, 4.5 to 7.5 percent of Maillard reactant, 8.5 to 11.5 percent of xylitol (liquid), 5.5 to 8.5 percent of natural juice acid, 2.5 to 5.5 percent of hops tincture, 2.5 to 5.5 percent of tobacco B-type extract, 0.1 to 0.5 percent of vanilline, 16 to 20 percent of propanediol, 5 to 8 percent of alcohol, 4.5 to 7.5 percent of drug flavor aromatic base and the balance of water. The drug flavor aromatic base comprises 20 to 30 percent of sage clary oil, 20 to 30 percent of pineapple ketone, 20 to 30 percent of guaiacol and 10 to 40 percent of alcohol.
Owner:飞嘉创业化工(深圳)有限公司

Catalyst for catalyzing hydrogenation deoxidation reaction of guaiacol and preparation method thereof

The invention provides a cheap catalyst for catalyzing the hydrogenation deoxidization reaction of guaiacol and a preparation method thereof. The catalyst comprises the following components: a carrier ZrO2-SiO2 binary composite oxide, and two active metals of Ni and Cu, wherein the two active metals of Ni and Cu are loaded on the binary composite oxide through an immersion process. The ZrO2-SiO2 binary composite oxide which is prepared through a chemical precipitation process is used as the carrier of the catalyst, wherein the Zr / Si molar ratio is 1:1-5; and the two active metals of Ni and Cu are loaded on the carrier through the immersion process, wherein the Ni load capacity is 7-15wt%, and the Ni / Cu molar ratio is 1:0.5-1.5. The catalyst prepared in the invention has the advantages of cheap raw material, simple preparation method, high reaction activity, good carbon deposition resistance, and long service life. According to the catalytic activity evaluation of the catalyst, the conversion rate of guaiacol is near to 100%, and the top alkane yield can reach 62.7%.
Owner:GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI

Kiwi growth cold resistant agent

The invention provides a kiwifruit growth cold-resistant agent, which is composed of the following materials based on weight percentage: 0.05-5% of 5-nitro guaiacol sodium, 0.3-5% of humic acid, 0.1-2% of paclobutrazol, 0.5-5% of chitosan, 0.1-5% of rosin, 0.1-5% of castor wax, 0-3% of paraffin, 0-5% of citric acid, 0-6% of calcium chloride, 0.5-10% of propylene glycol, 0.1-15% of salicylic acid, 0.5-8% of vegetable oil, 0.05-10% of dimethyl sulfoxide, 0.1-2% of sodium dodecyl benzene sulfonate, and 35-85% of water. The cold-resistant agent can effectively increase the membrane stability of kiwi branches, remove harmful free radicals, regulate in-vivo water balance of the plants, improve anti-freezing performance and ensure kiwifruit yield.
Owner:SICHUAN UNIV

Productive technology of vanlillin by glyoxylic acid method

The invention discloses a productive technology of vanlillin by a glyoxylic acid method. The productive technology comprises a synthesis process, a fractionation process and a purification process, wherein the synthesis process comprises condensation treatment, oxidation treatment and decarboxylation treatment of methyl catechol and glyoxylic acid. The productive technology particularly comprisesthe following steps: respectively converting the methyl catechol and the glyoxylic acid into guaiacol sodium and sodium glyoxylate in a sodium hydroxide system; carrying out condensation treatment onthe guaiacol sodium and the sodium glyoxylate; after recovering the unreacted methyl catechol in a condensation liquid, carrying out oxidation treatment, namely carrying out catalytic oxidation on anethanol group in 4-hydroxy-3-methoxybenzene sodium glycolate by using copper oxide in the sodium hydroxide system to form a ketone group, thereby generating a corresponding ketonic acid compound; after separating red copper oxide particles from an oxidation liquid, carrying out decarboxylation treatment, namely using sulfuric acid to acidize the oxidation liquid, and simultaneously converting an acid group in the ketonic acid compound into carbon dioxide so as to generate 4-hydroxy-3- methoxybenzaldehyde; and carrying out the fractionation process and the purification process to obtain the vanlillin.
Owner:喜孚狮王龙香料(宁波)有限公司

Spicy spice for kretek blasting bead, preparation method and application thereof

InactiveCN104804871ACover up bad breathMake up for distortionTobacco treatmentEssential-oils/perfumesIsoeugenolGuaiacol
The invention provides a spicy spice for a kretek blasting bead, a preparation method and application thereof. The spicy spice is characterized in that the spicy spice is prepared by mixing the following raw materials in percentage by weight: 2 to 5 percent of clove bud oil, 0.5 to 1 percent of eugenol, 0.01 to 0.03 percent of thymol, 0.2 to 0.8 percent of beta-caryophyllene, 0.2 to 0.8 percent of guaiacol, 0.5 to 1 percent of isoeugenol, 0.1 to 0.5 percent of vanillin, 0.5 to 1 percent of benzyl alcohol, and the balance of a solvent. The spicy spice can not only cover adverse chemical smell produced by wall materials and package solvent when the kretek blasting bead is pinched, but also provide spicy fragrance coordinated with the fragrance of the tobacco, makes up the situation of distortion of clove fragrance during burning of the kretek, and enables the kretek to have conformable and natural spicy fragrance.
Owner:HUBEI CHINA TOBACCO IND +2

Method for preparing aromatic compounds employing catalytic cracking of industrial lignins

The invention relates to a method for preparing aromatic compounds employing direct catalytic cracking of industrial lignins under a catalytic system with reducing capacity. The method comprises the following steps: selectively cracking industrial lignins such as sodium lignin sulfonate, alkali lignin, dealkalized lignin and the like as raw materials under combined action of a catalyst and a solvent with reducing capacity to obtain derived C6-C9 phenolic, guaiacol or syringyl aromatic compounds and the like by the effect of in-situ reduction of a poisoned catalyst, wherein the conversion rate of the raw materials can be over 50%; the content of guaiacol and syringyl aromatic compounds in a split product can be over 70%. By adopting the method related to the invention, the problem that the catalyst is poisoned by reaction materials is overcome, so that the catalytic process can be smoothly carried out; the method has the characteristics of low price and high conversion efficiency of raw materials, high yield of the aromatic compounds and mild reaction condition.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Se-enriched plant growth regulator

A Se-enriched plant growth regulator is an aqueous product prepared from the sodium (potassium or ammonium) selenate (or selenite) and the sodium (potassium or ammonium) salt of 5-nitroguaiacol, 2,4-dinitrophenol, or catechol. Its advantages are high chemical and physical stability, and high effect on increasing the content of organoselenium in grains of crops and promoting photosynthesis.
Owner:天津市农业技术推广站

Benzoxazine resin based on furfuryl amine, composition and preparation method thereof

The invention relates to a benzoxazine resin based on furfuryl amine, a composition and a preparation method thereof. The benzoxazine resin is prepared from phenol derived from renewable resources (for example, eugenol, guaiacol and cardanol), primary amine derived from renewable resources (for example, furfuryl amine) and an aqueous solution of paraformaldehyde or trioxymethylene or formaldehyde; and the relevant composition is a composition prepared from the benzoxazine resin and other thermosetting resins. The benzoxazine resin based on furfuryl amine and the composition thereof can be used in multiple fields of matrix resins of paint, thermosetting plastic, composite material and the like.
Owner:BEIJING UNIV OF CHEM TECH

Method for synthesizing guaiacol

The present invention is process of synthesizing guaiacol, and relates to the application of catalyst in catalytically synthesizing guaiacol through the solid phase alkylation reaction between catechol and methanol. The catalyst has active component of ammonium metatungstate, sodium tungstate, ammonium molybdenum, ammonium biphosphate, ammonium phosphate, ammonium chromate or silicotungstic acid; and carrier of active carbon, SiO2, ZnO, Al2O3, TiO2, BaCO3 or CaO; and active component supporting amount of 2.1-30.2 wt%. The process of synthesizing guaiacol has catalytic reaction temperature of 533-573 K, the molar ratio between catechol and methanol of 1 / 7-1 / 2.5, and catalyst amount of 0.84-2.8 weight portions. During the process, the mixed solution of catechol and methanol is fed to the pre-heater in the flow rate of 0.3-2ml / hr, gasified and reacted catalytically at the temperature of 533-573 K.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1

Root promoting agent suitable for camellia chrysantha burgeon cuttage

The rooting agent suitable for stem planting of golden camellia branch consists of 2, 4-dichloro phenoxyacetic acid, 3-indole butyric acid, sodium guaiacol mononitrate, sodium hydroxide and borax. It is diluted before being used for stem planting of golden camellia branch in spring, summer and autumn, and survival rate of golden camellia seedling may reach 93-98 %. The present invention makes it possible to protect, develop and utilize golden camellia via fast industrial seedling growing.
Owner:GUANGXI FUXIN SCI & TECH

Tobacco essence capable of improving cigarette taste and aroma

The invention relates to tobacco essence capable of improving cigarette taste and aroma and also relates to application of the tobacco essence in cigarettes. The tobacco essence mainly comprises propylene glycol, loquat leaf extract, salvia miltiorrhiza extract, labdanum oil, rhodinol, mint lactone, guaiacol, carvone, acetic acid isobutyl ester and alpha-pinene. The tobacco essence has the effects of softening and smoothening the smoke, cleaning the residual odor, reducing the irritation and moisturizing, and the tobacco quality is improved.
Owner:HUABAO FLAVOURS & FRAGRANCES CO LTD

Hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst, and method for preparing bio-oil through catalytic pyrolysis of lignin by catalyst

The invention discloses a hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst, and a method for preparing bio-oil through catalytic pyrolysis of lignin by the catalyst. The metal modified hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst is obtained through modifying an H-type hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst with metal, and the metal can be one or more of Ga, Ce,Ni, Fe, Zn and Co. The catalyst disclosed in the invention is obtained through isopyknic impregnation process modification of the one or more transition metals. The non-in situ analysis method for thecatalytic pyrolysis of lignin by the modified catalyst comprises the following steps: introducing the modified hierarchical pore molecular sieve catalyst into a fixed bed reactor, performing ectopiccatalytic pyrolysis on the lignin to prepare a functional chemical substance, and performing catalytic conversion separation and purification to obtain a liqid product mainly comprising phenol, guaiacol, hydroquinone and catechol.
Owner:HUIZHOU RES INST OF SUN YAT SEN UNIV

Anticorrosive paint

The invention discloses anticorrosive paint which comprises, by mass, the following materials of 2-5 parts of allyl heptanoate, 1-4 parts of 4-methyl guaiacol, 1-2 parts of acetylacetone selenium, 4-9 parts of polyamide resin, 2-4 parts of 3-chlorine-2-carboxyl propyl trimethyl ammonium chloride, 1-3 parts of chromic chloride, 3-7 parts of vinylidene chloride, 2-5 parts of tetrabutylammonium bromide, 0.2-0.5 part of yttrium oxide, and 3-6 parts of decabromodiphenyl ether. The anticorrosive paint is not only convenient to use, easy to produce and manufacture, good in painting performance, and good in wettability for object surfaces, but also rapid in paint film drying, good in mechanical capacity, and good in acid-base resistance property, water resisting property, and resistance to organic solvent.
Owner:NANTONG HAISHUN CHEM

Process for continuous hydrolysis synthesis of guaiacol

The invention provides a process for continuous hydrolysis synthesis of guaiacol consisting of, converting copper salt discharged from the hydrolysis kettle into alkali type copper, reacting the alkali type copper returning the hydrolysis kettle with sulphuric acid carried in by the diazonium liquid, so as to convert into copper sulfate again, thus realizing the circulation of copper sulfate catalyst.
Owner:浙江纵华香料股份有限公司

Method for producing guaiacol with methanol method

The invention provides a method for preparing guaiacol by a methanol method. Methanol used as a methylating agent and pyrocatechol are used for synthesizing the guaiacol under the action of a solid acid catalyst in a high pressure vessel. The solid acid catalyst used for synthesizing the guaiacol has low price, is friendly to environment and can be recycled. The invention is characterized in that the invention has simple preparation technology, mild reaction condition and short reaction time; the obtained guaiacol is light yellow clear transparent liquid and has good product quality, high yield, high selectivity and few side products; and all side products in reaction liquid can be recycled.
Owner:BEIJING UNIV OF CHEM TECH

Fertilizer and pesticide combination reagent for preventing and killing bacterial canker of kivifruit and preparation method of fertilizer and pesticide combination

The invention discloses a fertilizer and pesticide combination reagent for preventing and killing bacterial canker of kiwifruit and a preparation method of the fertilizer and pesticide combination reagent. The fertilizer and pesticide combination reagent is prepared from the following raw materials in parts by weight: 200 parts of agricultural amino acid water soluble powder, 40-50 parts of anhydrous zinc chloride, 45-65 parts of anhydrous manganese chloride, 40-80 parts of a ciprofloxacin active compound or a ciprofloxacin active compound, 100-200 parts of a 14% methyl chloride isothiazolinone water agent, 100-150 parts of oxine-copper active compound, 0.1 part of forchlorfenuron, 0.1 part of a 5-nitroguaiacol sodium salt active compound, 0.5 part of an alpha-pimacol active compound, 350-450 parts of molasses microbial fermentation concentrate solution, 0.2-0.25 part of sodium polyacrylate powder, 50-60 parts of No.YD-7 liquid fertilizer synergia solution, 0.1-0.2 part of industrial alcohol and the balance of deionized water to supplement the raw materials to 1200-1250 parts. The fertilizer and pesticide combination reagent disclosed by the invention is a high-density liquid product with fertilizer and pesticide effects, has good affinity for both water and plants, mainly contact and kill plant bacteria, and can also kill pathogenic fungus. A layer of compact organic pesticide and fertilizer film is formed outside a tree body when the fertilizer and pesticide combination reagent is applied in large concentration, and a sustained release function is exerted.
Owner:贵州三福生物科技有限公司 +1

High performance liquid chromatography determination method for volatile phenolic compounds in white spirits

The invention discloses a high performance liquid chromatography determination method for volatile phenolic compounds in white spirits. By adopting a high performance liquid chromatogram-fluorescence detector (HPLC-FLD) technique to analyze volatile phenolic compounds in white spirits, the method of the invention, when used in analysis of volatile phenolic materials, can determine the following 10 volatile phenolic compounds of phenol, guaiacol, p-cresol, m-cresol, o-cresol, 4-methylguaiacol, 4-vinylphenol, 4-ethylphenol, 4-vinylguaiacol and 4-ethylguaiacol in white spirits. And the method has the advantages of no need for derivatization, high accuracy, high sensitivity and good repeatability.
Owner:GUIZHOU PROVINCIAL PRODUCT QUALITY SUPERVISION AND INSPECTION INSTITUTE +1

Eugenol synthesis method

The invention belongs to the field of organic synthesis and particularly relates to a eugenol synthesis method. According to the eugenol synthesis method, guaiacol and allyl chloride are used as raw materials and are subjected to a catalytic reaction through a catalyst THLD to generate eugenol; an experiment testifies that the eugenol is synthesized by using the novel composite catalyst THLD, so that the conversion rate of the guaiacol and the yield of the eugenol are greatly improved, the conversion rate of the guaiacol reaches to 98 percent and the yield of the eugenol reaches to 88 percent.
Owner:CHONGQING THRIVE CHEM

Maple flavor formula

ActiveCN103704659AReduce harsh chemical smellIncrease burnt aromaFood ingredient as flavour affecting agentFood preparationFood additiveAdditive ingredient
The invention discloses a maple flavor formula, which is mainly composed of ethyl maltol, methyl cyclopentenolone, ethyl butyrate, furanone, vanillin, a caramel reactant, maltol, 2,3,5-trimethyl pyrazine, isoamyl alcohol and guaiacol. The maple flavor prepared by the formula has obvious advantages that caramel reactant obtained from natural reaction is used as the key material, and a matching formula is employed for preparation of the maple flavor. As a food additive used in food, the maple flavor can endow a product with a maple aroma and taste with natural sense; at the same time, the added natural caramel reactant reduces stiff chemical smell of a single aroma material for the product and increases maple taste, so that the quality and quantity of aroma are higher than those of the similar domestic essence; and the heat resistance and stability of the product are also higher than those of the similar domestic product.
Owner:HANGZHOU EVER MAPLE FLAVOR & FRAGRANCE

Method for synthesizing veratraldehyde

The invention discloses a method for synthesizing veratraldehyde. In the method, a methylation reaction and a formylation reaction are performed on catechol serving as a raw material in turn to generate the veratraldehyde, wherein the methylation reaction comprises two reaction stages: guaiacol is mainly generated at the first reaction stage, and the veratrole is generated at the second reaction stage; and during the formylation reaction, the veratrole and a formylation reagent are synthesized to obtain the veratraldehyde. Through comprehensive utilization of a methylation reagent and the formylation reagent, the current situation that the veratraldehyde can only be synthesized by expensive vanillin in the conventional industrial production is changed, the process route for synthesizing the veratraldehyde is effectively shortened, and the cost of a product is reduced; both the yield and the content of the veratraldehyde prepared by the method are greater than those of the veratraldehyde prepared in the prior art; due to the increase of the yield and the content of the veratraldehyde, the process for synthesizing the veratraldehyde is effectively controlled; thus the entire process flow is smooth and stable.
Owner:四川鸿康药物化学有限公司

Selenium-rich and disease and pest resistant compound fertilizer and manufacturing process thereof

The invention discloses selenium-rich and disease and pest resistant compound fertilizer. The selenium-rich and disease and pest resistant compound fertilizer is prepared from the following raw materials: monopotassium phosphate, controlled release nitrogen, grass carbon, soybean meal, sasanqua cake, rapeseed meal, amino acid, zinc sulphate, copper sulphate, ferrous sulphate, borax, 5- nitroguaiacol sodium salt, SiO2 soil improvement agent, selenium-rich yeast, alpha-pimacol, VB, VC and citric acid. The selenium-rich and disease and pest resistant compound fertilizer is scientific and rigorous in formula and is biofertilizer with selenium nutrition, resistance to disease and pest and pollution-free (low residue) effect, and a technology is scientific, organic, non-toxic, green and environmental-friendly. The selenium-rich and disease and pest resistant compound fertilizer can enhance a plant immunological function system, promote mobility of immune cells, regulate specific and non-specific immunological functions of a plant, effectively increase organic selenium content of the plant, realize a wound healing effect, rapidly promote new root development, cause the plant to be thick and strong, leaves to be thick, green, open and flat and root tubers and bulbs to be thickened and expanded, prevent premature senility and resist to cold, waterlogging, continuous cropping, salt and alkali.
Owner:辽宁丰园肥业有限公司

Monochamus alternatus attractant and application method thereof

The invention discloses a Monochamus alternatus attractant which comprises a plant-derived pheromone and an aggregation pheromone. The plant-derived pheromone comprises the following components in parts by volume: 60-75 parts of turpentine or alpha-pinene, 18-30 parts of ethanol, 0-5 parts of guaiacol and 0-5 parts of 4-methyl guaiacol. The aggregation pheromone is 10-100 mu L / lure 2-undecyloxy-1-ethanol. The application method comprises the following steps: the plant-derived pheromone is released by a slow-release bottle, wherein radial bulge lines are arranged in the cap of the slow-release bottle, the slow-release bottle is prepared from low-density polyethylene (LDPE) with the density of 0.910-0.925 g / cm<3>, and the wall thickness of the slow-release bottle is 0.20-0.70mm; the aggregation pheromone is released by a slow-release bag, wherein the slow-release bag is prepared from LDPE with the density of 0.910-0.925 g / cm<3>, and the wall thickness of the slow-release bag is 0.04-0.15mm. The average trap quantity per week of each Monochamus alternatus attractant is up to 59.01; the drug effect can stably stay for 2 months or so; and thus, the Monochamus alternatus attractant can greatly lower the population density of Monochamus alternatus and inhibit the pine wood nematode disease from propagation and transmission.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Bacteria ferulic acid decarboxylase gene and crystal structure thereof

The invention relates to an expression, purification, crystallization method, structure elucidation and the field of application technology of forulic acid decarboxylase, which belongs to the field of molecular biology and microbiology application. The invention obtains the coding area sequence of the key enzyme forulic acid decarboxylase which decomposes forulic acid to generate 4-vinyl guaiacol in Enterobacter sp.Px6-4 by the gene clone method, expresses the enzyme in a large amount in the colon bacillus ( E.coli BL21 ) by the heterogenous expressing technology, obtains the forulic acid decarboxylase pure products by the purification method and the forulic acid decarboxylase protein crystallization by the hanging-drop crystallization process, and grasps the structure and active centre of the enzyme. The invention can obtain a large amount of highly active forulic acid decarboxylase, and provide theoretical guidance for reconstructing engineering bacteria and improving the output of 4-vinyl guaiacol by analyzing the active centre of the enzyme, thus having good potential application.
Owner:YUNNAN UNIV

Method for fermentative production of laccase using fungoid co-cultivated

This invention relates to a epiphyte co-culture ferment Laccase production method, which is to inoculate the bacterium detergent of the Trametes AH28-2(Trametes sp. AH28-2) in the ferment culture medium under 22-37deg C for 1-4 days, then adding the bacterium detergent of Trichoderma ZH1(Trichoderma sp. ZH1) to culture for 5-10 days, finally separating to obtain the Laccase preparation, wherein the enzyme activity is up to 6, 000U / L(guaiacol method). The culture medium is the liquid culture medium, which mainly contains a certain quantity of carbon, azotes, phosphate of kalium and natrium, sulfate of copper and ferrum, adenine and vitamin B and so on. This invention doesn't' use the aromatic compound and heavy metal iron induce, which is safe and clean, and the enzyme activity is high and stable. '
Owner:ANHUI UNIVERSITY
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