50 results about "Laodelphax striatella" patented technology

The invention provides a method for preserving rice black-streaked dwarf virus (RBSDV) indoor living bodies. According to the principle of the method, wheat serves as a virus source host, small brown planthopper imagoes carrying the RBSDV spread the virus on wheat seedlings, a descendant small brown planthopper nymphs obtain the virus from the wheat, and accordingly a large of small brown planthoppers with the virus are obtained at a short period under indoor feeding conditions. The method effectively overcomes the technical bottleneck in preserving a large number of RBSDV indoor living bodies in a long term, and a series of technical problems that an RBSDV virus source is difficult to preserve continuously through the living bodies, the conventional cycle of obtaining the virus source is long, virus feeding plants cannot be provided for a long term, and minimum of the instar of the virus feeding nymphs is difficult to operate, and the like are solved. The obtained small brown planthoppers with the virus can support rice and corn variety resistance identification at any time and can also serve as RBSDV purification and serum preparation materials, and the problems that materials are difficult to obtain and the virus content is low and the like in conventional purification are solved. By application of the method, research, development and utilization of the RBSDV are greatly accelerated.

The invention belongs to the field of agricultural and biological technology and relates to a Laodelphax striatellus lethal gene fragment thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence represented by SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the maximum adjusted mortality of the Laodelphax striatellus may reach 67.4 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

The invention belongs to the technical field of agricultural biology, and in particular relates to gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered;the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the death rate of the laodelphax striatellus reaches 70%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using an RNA interference technology.

The invention belongs to the field of agricultural and biological technology and relates to a gene silencing technique based Laodelphax striatellus lethal gene fragment Alpha1-tubulin and a dsRNA thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Alpha1-tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence representedby SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the adjusted mortalityof the Laodelphax striatellus may reach 70 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

The invention discloses a rice suspension seed coating agent containing imidaclothiz and chlorantraniliprole. The rice suspension seed coating agent is characterized by mainly comprising main components, auxiliary agents and distilled water; the main components comprise the imidaclothiz and the chlorantraniliprole, wherein the imidaclothiz accounts for 10 to 40 mass percent of the rice suspension seed coating agent, and the chlorantraniliprole accounts for 2 to 10 mass percent of the rice suspension seed coating agent; the invention also discloses application of the rice suspension seed coating agent containing the imidaclothiz and the chlorantraniliprole; the rice suspension seed coating agent disclosed by the invention can effectively control the outbreak of laodelphax striatellus through one-time seed treatment in a seedling stage, and has a good prevention and control effect on stenchaetothrips biformis; through one-time seed treatment, cnaphalocrocis medinalis guenee in the early and medium stages of rice can be controlled basically, and the cnaphalocrocis medinalis guenee is basically not required to be prevented and controlled until the rupturing stage of the rice; the using amount and the using frequency of pesticides are obviously reduced, so that the prevention and control cost is reduced, much labour is saved, meanwhile, the environmental pollution is reduced, and pesticide residues of agricultural products are reduced; therefore, the rice suspension seed coating agent containing the imidaclothiz and the chlorantraniliprole can be widely applied to the field of rice seed treatment.

The invention provides a method for preserving rice black-streaked dwarf virus (RBSDV) indoor living bodies. According to the principle of the method, wheat serves as a virus source host, small brown planthopper imagoes carrying the RBSDV spread the virus on wheat seedlings, a descendant small brown planthopper nymphs obtain the virus from the wheat, and accordingly a large of small brown planthoppers with the virus are continuously obtained at a short period under indoor feeding conditions. The method effectively overcomes the technical bottleneck in preserving a large number of RBSDV indoor living bodies in a long term, and a series of technical problems that an RBSDV virus source is difficult to preserve continuously through the living bodies, the conventional cycle of obtaining the virus source is long, virus feeding plants cannot be provided for a long term, and minimum of the instar of the virus feeding nymphs is difficult to operate, and the like are solved. The obtained small brown planthoppers with the virus can support rice and corn variety resistance identification at any time and can also serve as RBSDV purification and serum preparation materials, and the problems that materials are difficult to obtain and the virus content is low and the like in conventional purification are solved. By application of the method, research, development and utilization of the RBSDV are greatly accelerated.

The invention discloses a lethal gene segment Ribosomal protein L9e of small brown planthopper and an application of the lethal gene segment. The lethal gene segment Ribosomal protein L9e, capable ofresulting in small brown planthopper death after interference and has the sequence represented as SEQ ID NO.1, of small brown planthopper is screened out, when dsRNA of the gene segment is utilized for feeding small brown planthopper and brown planthopper, the small brown planthopper and the brown planthopper can be effectively killed, the lethal gene segment is safe to ecological environment andfood, and a new way is provided for pest control with the RNA interference technology.

The invention discloses a trifluoropyrim-containing synergistic insecticide composition and an application thereof, belonging to the technical field of pesticides. The insecticide composition of the present invention comprises two active components A and B, wherein the active component A is trifluoropyrim, and the active component B is one of the botanical pesticides matrine, azadirachtin and pyrethrin. In the insecticide composition of the present invention, the action mechanism of the active ingredient A and the active ingredient B are completely different, and the combination can synergize the pests, improve the control effect, reduce the amount of pesticide use, delay the generation of drug resistance, and reduce the risk of drug resistance. It can be used for the control of piercing-sucking mouthparts such as wheat aphid, cotton aphid, corn aphid, bean aphid, potato aphid, apple cotton aphid, tomato whitefly, brown planthopper, brown planthopper, and green leafhopper.

The invention discloses an application of xanthoxylin as a rice planthopper repellent. The field use concentration of the xanthoxylin in prevention and treatment of rice planthoppers is 10-1000 ppm ofeffective components. Under a use concentration of 1000 ppm, the xanthoxylin can reach repelling rates of 81.21%, 77.50% and 65.23% to three kinds of female adult rice planthoppers, i.e., brown planthoppers, sogatella furcifera and laodelphax striatellus respectively after the xanthoxylin is sprayed for 24 hours; and the xanthoxylin has a strong repelling effect. The rice planthopper repellent compound, namely the xanthoxylin provided by the invention is an extraction component of natural plants; and a rice planthopper repellent prepared from the xanthoxylin has the advantages of good prevention and treatment effects, simple preparation, greenness, safety, effectiveness, environmental friendliness and the like.

The invention provides a method for detecting rice stripe virus in single-headed brown planthopper by using dot-ELISA, which belongs to the field of plant protection. In the invention, the monoclonal antibody of rice stripe virus is used to establish a dot-ELISA system for detecting the rice stripe virus carried by the single-headed medium, SBPH, after collecting and grinding the brown planthopper. The detection method has high sensitivity and strong specificity, is suitable for the detection of large quantities of single-headed SBPH samples, and does not require special equipment. It has the characteristics of simple result judgment and long-term storage of test results. Large-scale investigation and detection of the rate of rice stripe virus carried by lice, to provide technical support for the prediction of the outbreak of rice stripe disease in the field and the adoption of corresponding prevention and control measures.