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Method and artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses

A technology of rice streak virus and artificial feed, which is applied in the directions of viruses, animal feed, animal feed, etc., can solve the problems of the low rate of drug acquisition and restrict the work of drug acquisition, and achieves the goal of promoting the transmission mechanism and improving the rate of drug acquisition. Effect

Active Publication Date: 2016-07-13
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the traditional method of feeding SBPH with virus-infected strains has a relatively low acquisition rate of SBPH. In addition, the collection of virus-infected strains is restricted by seasonal factors, which restricts the research on the work of SBPH.

Method used

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  • Method and artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses
  • Method and artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses
  • Method and artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the preparation of the artificial feed that contains rice stripe virus

[0036] 1. Crude extraction of rice stripe virus

[0037] 1. Take 1 g of rice leaves carrying rice stripe virus collected in the field and collected at -80 ° C. Grind it into a fine powder with liquid nitrogen, add 3 ml of PBS buffer solution (the solvent is water, the solute and its concentration are as follows: dihydrogen phosphate Potassium (KH 2 PO 4 )0.2g / L, disodium hydrogen phosphate dodecahydrate (Na2HPO 4 12H 2 O) 2.9g / L, sodium chloride (NaCl) 8.0g / L, potassium chloride (KCl) 0.2g / L; pH 7.4), 4°C, shake with a rotator-mixer for 15min.

[0038] 2. Centrifuge at 12,000 rpm (equivalent to 15,294 g) at 4°C for 10 min, and take the supernatant.

[0039] 3. Sterilization by filtration: filter the supernatant obtained in step 2 with a syringe filter (Acrodisc Syringe filter 0.22 μm supermembrane low protein binding Non-pyrogenic) with a pore size of 0.22 μm, and take the filtra...

Embodiment 2

[0074] Embodiment 2, make the small planthopper obtain rice stripe virus by film feeding method

[0075] 1. To attack poison:

[0076] Get three parts of two-year-old non-toxic SBPH, each of which is dozens, and place them in three identical glass tubes (12 cm long and 2.8 cm in diameter) with open ends, and seal one end of the glass tube with a double layer of air-permeable air. membrane (air-permeable parafilm film), the other end is first sealed with a layer of air-permeable sealing film, with the sterile side facing outward, and the artificial feed is added to the film, and then the sterile side of another layer of air-permeable sealing film is facing inward to seal the feed , put 70 μ l of the artificial feed containing RSV prepared in Example 1 in the middle (corresponding to the three glass tubes respectively adopting three groups of artificial feeds of A, B, and C in Table 1), the glass tubes are placed horizontally so that the two layers The surface of the artificial...

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Abstract

The invention discloses a method and an artificial feed for enabling laodelphax striatellus to obtain rice stripe viruses. The method for enabling rice stripe viruses to obtain laodelphax striatellus comprises the following step: feeding the laodelphax striatellus with the artificial feed containing rice stripe viruses, thereby enabling the laodelphax striatellus to obtain rice stripe viruses. The composition of the artificial feed is that every 1 muL of the artificial feed contains the rice stripe viruses greater than or equal to 300ng, the balance being a sucrose aqueous solution with concentration being 50g / L. According to the method, the rice stripe virus acquiring rate is higher than 90% which is greatly increased in comparison with that of a conventional method of feeding non-toxic ash-free plant hoppers by toxic rice plants. According to the method, an experimental platform for enabling the laodelphax striatellus to obtain toxin efficiently is established, and the research on the transmission mechanism of rice stripe viruses in the laodelphax striatellus is strongly promoted, and the effective progress of the novel disease-resistant strategy of removing virus in insects is promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for obtaining rice stripe virus and artificial feed for the planthopper. Background technique [0002] Rice stripe blight is one of the major rice diseases in my country. Diseased plants often withered and booted or the ears were small and deformed. After jointing, yellow-green stripes appear on the lower part of the flag leaf. All types of rice are not dry, but the heading is deformed and the fruit is few. The pathogen is rice stripe virus (Ricestripevirus, RSV), which belongs to the virus of the genus Levovirus. Virus particles are filamentous, with a size of 400×8nm, dispersed in the cytoplasm, vacuoles, and nuclei, or form amorphous aggregates such as granules and sand, that is, inclusion bodies, which seem to be entangled with many filaments. The dilution limit of diseased leaf juice is 1,000 to 10,000 times, the passivation temperature is 55°C for 3 minutes, minus 20°...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K10/30A23K20/163A23K10/18A23K50/90C12N7/00
CPCA01K67/033C12N7/00C12N2720/00051
Inventor 陈丽莹霍岩张莉莉
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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