Application of Zanthoxylum oil as rice planthopper repellant
A technology of Zanthoxylum bungeanum oil and rice planthopper, which is applied in the direction of animal repellent, application, pest repellent, etc., can solve the problems that the application has not been reported, and achieve the effect of simple preparation, environmental friendliness and good repellent effect
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Embodiment 1
[0027] Example 1: Determination of the repellent activity of prickly ash oil against brown planthopper and planthopper natural enemy Lygus spp.
[0028] 1.1 Materials and methods
[0029] The tested insects were indoor populations of brown planthopper and green Lygus black-shouldered. Nilaparvata lugens took 3-instar nymphs and 7-day-old female adults to conduct repellent tests, and green Lygus black-shouldered nymphs took 3-instar nymphs and 7-day-old male and female adults to repel respectively. Test; the test compound is Zanthoxylum oleifera, which was purchased from Tokyo Chemical Industry Co., Ltd., Japan. The control repellent is the ethanol extract of Zanthoxylum bungeanum extracted in the laboratory, which is a soluble oily concentrate.
[0030] Bioactivity assay method:
[0031] The repellent activity was measured by stem spray method, see figure 1 . Two rice plants with a height of 30 cm are transplanted into a flower pot with a diameter of 18 cm at a distance of...
Embodiment 2
[0042] Example 2: Determination of the repellent activity of prickly ash oil to white-backed planthopper
[0043] 2.1 Materials and methods
[0044]The tested insects were indoor populations of white-backed planthoppers, and the 3rd-instar nymphs and 7-day-old female adults were used for repellent tests; the test compound was Zanthoxylum oleifera, which was purchased from Tokyo Chemical Industry Co., Ltd., Japan.
[0045] Bioactivity assay method:
[0046] The relevant repellent activity was determined by the stem spray method, and the relevant repellent activity test device is shown in figure 1 . Two rice plants with a height of 30 cm are transplanted into a flower pot with a diameter of 18 cm at a distance of 12 cm, and a transparent cylindrical polyvinyl chloride bucket with openings at both ends is placed on it. Take an appropriate amount of Zanthoxylum oleifera, add acetone to dissolve it in a 1:1 mass ratio, add Tween 20 to make a 20mg / ml Zanthoxylum bungeanum mother ...
Embodiment 3
[0055] Example 3: Determination of the repellent activity of prickly ash oil to SBPH
[0056] 3.1 Materials and methods
[0057] The tested insects were indoor populations of SBPH, and the 3rd-instar nymphs and 7-day-old female adults were used for the repellent test; the test compound Zanthoxylum oleifera was purchased from Tokyo Chemical Industry Co., Ltd., Japan.
[0058] Bioactivity assay method:
[0059] The relevant repellent activity was determined by the stem spray method, and the relevant repellent activity test device is shown in figure 1 . Two rice plants with a height of 30 cm are transplanted into a flower pot with a diameter of 18 cm at a distance of 12 cm, and a transparent cylindrical polyvinyl chloride bucket with openings at both ends is placed on it. Take an appropriate amount of Zanthoxylum oleifera, add acetone to dissolve it in a 1:1 mass ratio, add Tween 20 to make a 20mg / ml Zanthoxylum bungeanum mother solution containing 1wt% Tween 20, and then add ...
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