54results about How to "Significant lethal effect" patented technology

Belonging to the field of agricultural biotechnology, the invention relates to a laodelphax striatellus lethal gene fragment. By a laboratory improved dsRNA feeding method, the invention, from laodelphax striatellus, screens out the laodelphax striatellus lethal gene fragment Ribosomal protein L9-B which can lead to laodelphax striatellus death and has sequences as shown in SEQ ID No.1. Feeding laodelphax striatellus with the dsRNA of the gene fragment provided in the invention can have good lethal effect, thus providing sequence and data bases for establishing new strategies of pest control with RNA interference technology.

The invention belongs to the field of agricultural and biological technology and relates to a gene silencing technique based Laodelphax striatellus lethal gene fragment Alpha1-tubulin and a dsRNA thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Alpha1-tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence representedby SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the adjusted mortalityof the Laodelphax striatellus may reach 70 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

The invention belongs to the field of agricultural and biological technology and relates to a Laodelphax striatellus lethal gene fragment thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence represented by SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the maximum adjusted mortality of the Laodelphax striatellus may reach 67.4 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

Shot for shotgun cartridges is made form finely divided particles of dense metal such as a mixture of tungsten and molybdenum, bound by a matrix which may comprise ethylene propylene copolymer, or a blend of a terpolymer of ethylene, acrylic ester and maleic anhydride or an ionomer either alone or blended with such terpolymer or with a stock polymer material or a blend of polymers. Shot may be produced from such material by forming it into a strip, web or strand which is passed between aligned rollers with cooperating hemispherical indentations and thereafter punching the resulting shot from the resulting thin web.

The invention belongs to the technical field of agricultural biology, and in particular relates to a gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered; the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the corrected death rate of the laodelphax striatellus reaches more than 65%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using a RNA interference technology.

The invention belongs to the technical field of agricultural biology, and in particular relates to gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered; the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the death rate of the laodelphax striatellus reaches 70%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using an RNA interference technology.

A hybrid gun device composed of two barrels (1,10) that accept energy from combustion of standard propellant (6), one barrel (10) being operative to produce a high intensity electric current to add accelerating energy to a projectile (7) in the second barrel (1) and at least one coil (8) stage to convert energy between electrical and kinetic to cause the projectile (7) to be launched at hypervelocity.

The invention belongs to the technical field of agricultural biology, and in particular relates to gene silencing technology-based lethal gene fragment Chitinase 7 of laodelphax striatellus and dsRNA (double-stranded RNA) thereof. In the patented invention, a dsRNA feeding method improved in laboratories is used for screening out the lethal gene fragment Chitinase 7 of the laodelphax striatellus as shown in SEQ ID NO. 1 from laodelphax striatellus, wherein the lethal gene fragment Chitinase 7 of the laodelphax striatellus can lead to the death of laodelphax striatellus after being interfered;the dsRNA of the gene fragment is used for feeding the laodelphax striatellus so as to ensure that the death rate of the laodelphax striatellus reaches 70%; and the lethal gene fragment Chitinase 7 and the dsRNA thereof provide a sequence and data basis for establishing a new strategy of controlling injurious insects by using an RNA interference technology.

The invention discloses a culture medium. The culture medium comprises fragrant-flowered garlic extracting liquor and nutrient soil. The culture medium has the advantages that the culture medium is adopted to carry out cultivation, so that occurrence of root-knot nematode diseases can be remarkably lowered; besides, operation is simple and convenient and economic cost is lower; the culture medium is applied to preventing and controlling the root-knot nematode diseases, so that not only is environmental pollution caused by applying a chemical agent to prevent and control in the prior art avoided for achieving high efficiency, environmental protection and no harm, but also the defect that economic income is severely affected by a long prevention and control period, regional limitation, and the like, caused by rotation planting of the fragrant-flowered garlic is overcome, and normal growth of plants is kept, so that yield of plant products is improved, product quality is improved, higher economic value is brought, and therefore, the culture medium is worthy of large-area popularization and application.

The invention relates to an application of a cynanchum glaucescens extract in controlling psylla chinensis, belonging to the field of biological control of plant sources. Control effects of cynanchum glaucescens extract water aqua on overwintering psylla chinensis adults are preliminarily studied by using indoor toxicity measurement and field trial methods. Researches show that the cynanchum glaucescens extract water aqua in different concentrations has remarkable lethal effect on the overwintering psylla chinensis adults; after the overwintering psylla chinensis adults are treated for 24 hours, the field control effect of 0.07mg / mL of cynanchum glaucescens extract water aqua on psylla chinensis adults reaches 66.8%, and the indoor biological measurement effect reaches 100%; and the concentration of a formula is positively associated with the lethal effect on the overwintering psylla chinensis adults. Research results provide a green control medicament and technology for control of the overwintering psylla chinensis adults in pear orchard.

Belonging to the field of agricultural biotechnology, the invention relates to a laodelphax striatellus lethal gene fragment ADP-ribosylation factor based on gene-silencing technology and dsRNA thereof. By a laboratory improved dsRNA feeding method, the invention, from laodelphax striatellus, screens out the laodelphax striatellus lethal gene fragment ADP-ribosylation factor which can lead to laodelphax striatellus death and has sequences as shown in SEQ ID No.1. Feeding laodelphax striatellus with the dsRNA of the gene fragment provided in the invention can have good lethal effect, thus providing sequence and data bases for establishing new strategies of pest control with RNA interference technology.

The invention discloses gene sequences of inwardly rectifying potassium channels Kir1 of brown rice planthopper and beet armyworm and a high-throughput insecticide screening method established by using the channels. The genes Kir1 of the inwardly rectifying potassium channels disclosed by the invention are obtained by cloning hemipterous insects and lepidoptera insects for the first time; the Kir1 channels are closely related to regulation and control of physiological processes such as herbivory and excretion of insects; and the inhibition of the channels possibly affects the herbivory and excretion process of the insects or even causes the death of the insects, and therefore, the channels can be developed as a novel insecticide target. The Kir1 genes are integrated into an expression vector, are expressed in cells by transfection and are continuously screened to obtain a monocolonal cell line for stably expressing the Kir1; the monocolonal cell line can be used for stably expressing the Kir1 channels with the inwardly rectifying characteristic; and the influence of a tested compound on potassium fluxes of the Kir1 channels is determined by using a thallium fluorescent reagent according to the equivalence of thallium ions and potassium ions passing through the Kir1 channels, and further a potential insecticide having inhibitory activity for the Kir1 channels are screened from a plurality of candidate compounds.

The invention discloses apolygus lucorum G protein coupled receptor kinase. The amino acid sequence of the apolygus lucorum G protein coupled receptor kinase is shown as SEQ ID NO: 2. The invention also discloses a gene for encoding the apolygus lucorum G protein coupled receptor kinase as well as an acquisition method and application thereof. The invention further discloses dsRNA of the apolygus lucorum GRK gene and application thereof. The dsRNA of the synthesized GRK gene has a remarkable lethal effect on the apolygus lucorum, the population number of the apolygus lucorum is effectively controlled, and a new way is provided for pest control. Meanwhile, the dsRNA is synthesized by using escherichia coli, so that a product can be synthesized in a large scale within a short time, and the defect that a kit cannot synthesize a large amount of dsRNA is overcome. Meanwhile, the apolygus lucorum G protein coupled receptor kinase is low in cost, wide in application range, easy to operate, capable of being repeatedly used and free of limitation.

The invention provides a monoclonal antibody against CD19. The monoclonal antibody is characterized in that a complementary determining region (CDR) of a VH chain has amino acid sequences selected from the following groups: a heavy chain CDR1 shown as SEQ ID NO: 3, a heavy chain CDR2 shown as SEQ ID NO: 4 and a heavy chain CDR3 shown as SEQ ID NO: 5; a complementary determining region (CDR) of a VL chain has amino acid sequences selected from the following groups: a light chain CDR1 shown as SEQ ID NO: 6, a light chain CDR2 shown as SEQ ID NO: 7 and a light chain CDR3 shown as SEQ ID NO: 8. The invention further provides a preparation method and application of the monoclonal antibody. The monoclonal antibody can specifically recognize human CD19 proteins on the cell surface, is combined with the human CD19 by using 1*10<-7> M or lower KD and has the advantages that the immunogenicity is low, the HAMA reaction is not caused, and the like.

The invention discloses a chilo suppressalis lethal gene csu-miR-9b and application thereof. The sequence of the chilo suppressalis lethal gene csu-miR-9b is shown as 5'-UCUUUGGUUAUCUAGCUGUAUGA-3'. The invention discloses application of the csu-miR-9b in preparing a chilo suppressalis insecticidal reagent. On the basis of complete miRNA bioinformatics analysis, an effective miRNA, namely csu-miR-9b, is searched from mass data in a small RNA library. The csu-miR-9b synthesized in vitro, injected to chilo suppressalis, has a significant lethal effect on the chilo suppressalis, showing that the miRNA can serve as an effective interferent for important genes of the chilo suppressalis.

The invention provides new application of potassium formate for preventing and curing southern root knot nematode. The new application is that the potassium formate is used for preparing a southern root knot nematode pesticide; in the pesticide, the potassium formate can be taken as an active ingredient alone and can also be compatible with other ingredients for preventing and curing the southern root knot nematode together as an active ingredient. The pesticide is a potassium formate solution which is safe, non-toxic and efficient when used for preventing and curing the southern root knot nematode, and the egg incubation inhibition rate of the southern root knot nematode can reach 60%-90% or above when the concentration of the potassium formate solution is 4.00-10.00 mM / L; the second instar larvae fatality rate range of the southern root knot nematode is 10%-30% when the concentration of the potassium formate solution is 4.00-10.00 mM / L.

The invention relates to preparation and application of recombinant Mycobacterium smegmatis expressing NY-ESO-1. Specifically, the invention provides a plasmid for expressing a recombinant cancer-testis antigen NY-ESO-1 protein; and the plasmid comprises a beta-galactosidase signal peptide coding sequence or a beta- galactosidase coding sequence, and an NY-ESO-1 coding sequence. The invention also provides a recombinant Mycobacterium smegmatis containing the plasmid provided by the invention and its composition, and application thereof. The recombinant Mycobacterium smegmatis provided by the invention has significant killing effect on tumor cells expressing NY-ESO-1; therefore the recombinant Mycobacterium smegmatis can be used in effective prevention and / or treatment of the tumors.

The invention relates to application of a nano composite material in degradation of organic toxic molecules and sterilization, belongs to the technical field of antibacterial agents and pollution control, and in particular relates to a nano composite material Ag / AgCl / PDDA / GE formed by modifying silver (Ag) loaded on graphene by using phthalic acid diethylene glycol diacrylic ester (PDDA). Due to the good charge conductivity of graphene and the property that PDDA has positive charges, the catalysis activity and the antibacterial property of the material are improved; the experiment that H2O2 or O2 in air is catalyzed by using Ag / AgCl / PDDA / GE so as to degrade organic toxic molecules shows that the nano material is not only capable of catalyzing H2O2 to degrade the organic toxic molecules within a short time, but also capable of catalyzing oxygen in the air to degrade the organic toxic molecules; the sterilization experiment on ordinary representative pathogenic bacteria and tolerant bacteria shows that the nano material has remarkable lethal effects on gram positive pathogenic bacteria, gram negative pathogenic bacteria and some resistance bacteria.

The invention discloses a chilo suppressalis lethal gene csu-miR-260 and application thereof. The sequence of the chilo suppressalis lethal gene csu-miR-260 is shown as 5'-UUUUGGAUGACUGGCCCAUGUCGGCGU-3'. The invention discloses application of the csu-miR-260 in preparing a chilo suppressalis insecticidal reagent. On the basis of complete miRNA bioinformatics analysis, an effective miRNA, namely csu-miR-260, is searched from mass data in a small RNA library. The csu-miR-260 synthesized in vitro, injected to chilo suppressalis, has a significant lethal effect on the chilo suppressalis, showing that the miRNA can serve as an effective interferent for important genes of the chilo suppressalis.

The invention discloses meperfluthrin and propoxur compound effervescent tablets, and a preparation method and an application thereof. The effervescent tablets are prepared by compounding a raw medicine component and an auxiliary component, wherein the raw medicine component accounts for 10%-22% of the total weight of the effervescent tablets, and the auxiliary component accounts for 78%-90% of the total weight of the effervescent tablets; the raw medicine component is prepared by compounding a component A and a component B according to the weight ratio of 1:(5-50), wherein the component A isa pyrethroid compound represented by the formula A, and the component B is propoxur represented by the formula B; the auxiliary component includes a nano inclusion complex carrier, an acid source, a carbon dioxide source, a binder, a wetting dispersant, a disintegration agent, a lubricant and the like. The insecticidal effervescent tablet has the advantages of insecticidal spectrum expansion, efficiency increasing, easy storage and use and the like.

The invention belongs to the technical field of agricultural biology, particularly relates to miRNA capable of regulating lepidoptera pest death and delaying growth and development of lepidoptera pests as well as an analogue and an inhibitor of the miRNA, and further discloses application of the miRNA to preparation of medicaments for preventing and treating lepidoptera pests. According to the present invention, the sequence of a lepidoptera insect miRNA gene family is analyzed to obtain the lepidoptera insect plutella xylostella miRNA, i.e., pxy-miR-34-5p, and the mature body sequence of the pxy-miR-34-5p is further labeled and chemically modified so as to synthesize the corresponding miRNA analogue agomiR-34 and the inhibitor anti-agomiR-34 in vitro. A microinjection method is used for researching and finding that the analogue agomiR-34 and the inhibitor agomiR-34 of the pxy-miR-34-5p can delay the growth and development of lepidoptera pests or reduce the survival rate of lepidoptera pests by regulating and controlling the EcR of lepidoptera pests, and theoretical guidance is provided for establishing a new strategy for controlling lepidoptera pests based on the miRNA interference technology.

The invention relates to the field of a biotechnology, specifically to a method for controlling leaf mites by silencing an energy metabolism gene. The method provided by the invention comprises the following steps: leaf mite lethal gene AK is selected as an RNAi target gene; and the RNAi target gene is connected to a plasmid vector L4440 and is transformed to escherichia coli HT115 so as to obtain a bacterial strain for expression of AK gene dsRNA. The method is an important supplement for a leaf mite control method by mainly using a pesticide. By the RNAi technology, expression of the energy metabolism gene in leaf mites is interfered. The method provides a technical support for controlling leaf mites.

The invention relates to a termite luring and killing medicament and a preparation method thereof. The invention relates to the technical field of termite control. Calculated according to parts by mass, the termite luring and killing medicament specifically comprises the following raw materials: 0.5-2.5 parts of asarum, 1-2 parts of xanthium sibiricum, 0.5-1.5 parts of artemisia apiacea, 2-5 partsof camphor tree leaves, 2-5 parts of melia azedarach, 0.5-1 part of litsea cubeba, 0.1-0.5 part of lindera glauca, 0.5-0.8 part of croton, 0.3-1.2 parts of castor, 1.5-2.5 parts of tung oil tree, 2-3parts of biological additives and 50 parts of baits. The termite luring and killing medicament is developed by utilizing plants, the plants have the characteristics of environmental friendliness, easiness in degradation and harmlessness to human bodies, and various plants are compounded to poison, inhibit pupation and eclosion, sterilize, hinder growth and development and the like on termites, sothat the termite killing effect is improved.

The invention discloses application of a brown planthopper NCS2 gene or an encoded protein as a target spot in preparation of a drug for preventing and treating brown planthopper, and relates to the technical field of plant genetic engineering. According to the application disclosed by the invention, the effect of the rice pest nilaparvata lugens NCS2 gene in prevention and treatment of nilaparvata lugens is found, and the nilaparvata lugens has an efficient RNAi effect, so that the expression of the nilaparvata lugens NCS2 gene in a pest body can be interfered by utilizing the advantage of the nilaparvata lugens NCS2 gene. DsRNA of the NCS2 gene is obtained through an RNAi technology, and after the NCS2 gene is silenced in a microinjection mode, the transcriptional level of the NCS2 gene is reduced, individual development and ovary development of the brown planthopper can be effectively inhibited, the egg laying amount can be reduced, and the ability of inhibiting growth, development and reproduction of pests is achieved, so that reproduction of the brown planthopper is inhibited, and prevention and treatment of the brown planthopper are achieved. When NCS2 gene function research is carried out, it is found that an obvious lethal effect is generated when an RNAi technology is used for interfering expression of the gene in 2-5-age nymphs of brown planthopper.

The present invention relates to a pear psylla control medicament and application thereof in control of pear psylla, and belongs to the technical field of biotechnology pesticides. The pear psylla control medicament has significant lethal effects on pear psylla, compared with traditional pear psylla control methods, pharmaceutical ingredients of the pear psylla control medicament are derived from chenopodium ambrosioides, after use, the pear psylla control medicament is free of drug residue, can protect the environment, does not kill orchard pear psylla natural enemies, maintains ecological balance, is low in production cost, and facilitates extensive use in orchard pear psylla control. Research results provide a basis for further research and promotion of biological agents of chenopodium ambrosioides, and have far-reaching significance on long-term development of the ecological environment, human health and comprehensive management of diseases.