61 results about "MiRNA Gene" patented technology

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA397a genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA397a genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA397a gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA397a gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

The present invention relates to miRNA barrier technology, belonging to biological technology. The miRNA technology can be implemented according to the following steps: 1) determining the target gene sequence of combining mRNA 3' UTR and miRNA, 2) synthesizing specific anti-sense oligo-barrier sequence, 3) transducing specific anti-sense oligo-barrier sequence into cell and combining target mRNA to form diploid, which can barrier the target miRNA of mRNA. 5 nucleotides or deoxyadenylic acids positioned at two ends of the specific anti-sense oligo-barrier sequence of the step 2) are locked while ribose ring is bridge-linked by 2' oxygen atom and 4' carbon atom. The inventive method has property of good transduced gene stability, high expression. The present invention proves that it is practical for interfering miRNA function using gene specific mode and provides novel measure for researching miRNA function which enable the selective gene therapy strategy and lay foundation to miRNA gene-specific use.

The invention relates to a method for quickly constructing an Arabidopsis thaliana artificial miRNA gene interference vector, which completes the construction of the Arabidopsis thaliana silencing vector, mainly for modifying the silencing segment for different genes, and the other parts are the same, expanding the unchanged DNA on the target carrier In the interval, the invariant regions at both ends of the silencing-related fragments are put into the transgenic target vector in advance, and then the core silencing fragments are connected into the transformed vector to directly complete the vector construction. When selecting the head and tail fragments, the DNA sequence was analyzed, so that after the head and tail fragments were connected by a bridge fragment, a StuI restriction site and a SnaBI restriction site could be obtained. After transforming E. coli, the vector could be prepared in large quantities and used with StuI and The SnaBI enzyme endonuclease linearizes it, and directly connects the core silencing fragment synthesized by this patent method to complete the construction of the Arabidopsis thaliana silencing vector. The beneficial effects of the present invention are: simple and easy to operate, convenient operation, high benefit, can greatly shorten the experiment time, and reduce the transformation cost of a single silencing carrier.

The invention relates to the field of molecular biology, in particular to a dual-gene micro RNA (miRNA) fluorescent quantitative internal reference and application of a primer in shellfish development samples. The two genes (miR-71 and miR-8) are selected by the strict internal reference screening procedure and are the most stable miRNA genes in different development stages of shellfish miRNA, the expression stability of the two genes in different development stages is superior to that of common miRNA internal reference genes snRNA U6, snRNA U1 and 5.8S RNA. Two miRNAs are selected randomly, the dual-gene miRNA is used as the internal reference for carrying out fluorescent quantitative analysis in development samples, the expression results are highly accordance with the miRNA sequencing expression profile, and the reliability and the stability of the dual-gene internal reference are proved.

The invention discloses a method for predicting bacterial blight of rice by utilizing miRNA 169h genes, belonging to the technical field of biology. The method comprises the following steps: selecting rice to be detected and control rice, wherein the selected control rice is not infected with the bacterial blight but is cultivated with the rice to be detected under the same conditions; respectively separating total miRNA genes in the rice to be detected and the control rice; respectively detecting the expression quantities of the miRNA169h genes in the total miRNA genes in the rice to be detected and the control rice, wherein the sequences of the miRNA169h genes are shown in SEQ ID NO:1 in a sequence table; and judging whether the experiments are successful according to the expression quantities of the miRNA169h genes in the rice to be detected and the control rice and further judging whether a plant to be detected is infected with the bacterial blight if the experiments are successful. The prediction method disclosed by the invention achieves success, can achieve accurate prediction a few days before the bacterial blight of rice appears and has the effects of gaining time for early prevention and treatment and reducing the rice loss caused by the bacterial blight.

The invention relates to data mining in bioinformatics, and particularly to mining of disease bioinformatics data and miRNA gene data. Specifically, the invention relates to prediction of the potential association between miRNA and a disease through data mining of miRNA and disease biological information. The method provided by the invention comprises the steps of processing of miRNA and disease-related data; analysis of disease similarity; analysis of miRNA similarity; learning of domain-based miRNA and the disease; constraint probability matrix decomposition of disease-related miRNA; and prediction of potential association between miRNA of the disease. The method provided by the invention can be used for predicting the potential association between miRNA and the disease, and can detect potential miRNA associated with the disease.

The invention discloses a method for forecasting bacterial leaf blight of paddy rice by utilizing miRNA396c gene at early stage, and belongs to the technical field of biology. The method comprises the following steps: selecting to-be tested paddy rice and control paddy rice, wherein the selected control paddy rice is paddy rice which is not infected by bacterial leaf blight and is cultivated under same conditions with the to-be tested paddy rice; respectively separating total miRNA gene of the to-be tested paddy rice and the control paddy rice; respectively detecting the expression level of miRNA396c gene in the total miRNA gene of the to-be tested paddy rice and the control paddy rice, wherein the sequence of the miRNA396c gene is shown as SEQ ID NO: 1; and determining whether the experiment is successful according to the expression level of miRNA396c gene in the to-be tested paddy rice and the control paddy rice, if successful, further determining whether the to-be tested plant is infected by bacterial leaf blight. The provided prediction method is successful, is capable of accurately performing forecast several days before paddy rice has symptoms of bacterial leaf blight, wins time for early prevention and early controlling, and helps to reduce loss of paddy rice caused by bacterial leaf blight.

Medulloblastoma (MB) is the most common malignant brain tumor of children. To identify the genetic alterations in this tumor type, we searched for copy number alterations using high density microarrays and sequenced all known protein-coding genes and miRNA genes using Sanger sequencing. We found that, on average, each tumor had 11 gene alterations, markedly fewer than in common adult cancers. In addition to alterations in the Hedgehog and Wnt pathways, our analysis led to the discovery of genes not previously known to be altered in MBs. Most notably, inactivating mutations of the histone H3K4 trimethylase genes MLL2 or MLL3 were identified in 16% of MB patients. These results demonstrate key differences between the genetic landscapes of adult and childhood cancers, highlight dysregulation of developmental pathways as an important mechanism underlying MBs, and identify a role for a specific type of histone methylation in human tumorigenesis.

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA1318 genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA1318 genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA1318 gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA1318 gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

The invention relates to the field of biology, and particularly relates to application of dual-genetic microRNA (miRNA) fluorescent quantitative internal references and a primer thereof in shellfish tissue samples. Two genes (let-7 and miR-87) selected by the invention are selected according a strict internal reference screening process, and are miRNA genes with the most stable expression in the shellfish microRNA in different tissues; the expression stability of the genes in the tissue is superior to the common miRNA internal reference genes snRNA U6, snRNA U1 and 5.8S RNA. Two miRNAs are selected at random, and the dual-genetic miRNAs are taken as internal references to carry out fluorescence quantitative analysis in the tissue samples; the obtained expression result is highly identical to miRNA sequencing expression profile; the reliability and the stability of the dual genetic internal reference are proved.

The invention discloses a method for early and accurate prediction of rice bacterial blight by using miRNA162b gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA162b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA162b gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA162b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA172a genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA172a genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA172a gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA172a gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

The invention discloses the lethal gene csu-miR-9b of Chilo suppressalis and its application. The lethal gene csu-miR-9b of C. suppressalis borer, the sequence is 5'-UCUUUGGUUAUCUAGCUGUAUGA-3'. The application of the csu-miR-9b of the present invention in the preparation of an insecticidal agent for Chilo suppressalis. The present invention uses complete miRNA bioinformatics analysis to search for an effective miRNA—csu-miR-9b from a large amount of small RNA library data. Injection of C. suppressalis with csu-miR-9b synthesized in vitro has obvious lethal effect on C. suppressalis, indicating that the miRNA gene can be used as an effective interferer of important genes in C. suppressalis.

The invention discloses a method for predicting rice bacterial blight with miRNA169h gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA169h gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA169h gene is as shown in SEQ ID NO:1 in the sequence table According to the expression levels of the miRNA169h gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses an identification method for rice bacterial blight resistance, belonging to the field of biotechnology. The method comprises: inoculating the rice to be tested and the susceptible rice; isolating the miRNA gene in the rice to be tested and the susceptible rice; detecting the expression of the miRNA1425 gene locus; and judging whether the plant to be tested is resistant to bacterial blight. The application of the miRNA1425 gene site in identifying rice bacterial blight resistance. The present invention uses the transcription of the miRNA1425 gene locus for the first time to identify whether rice has bacterial blight resistance, and has achieved success. The transcription of the miRNA1425 gene locus provided by the invention is based on the detection of the expression level, avoiding the detection caused by white leaf blight. The misjudgment caused by the silencing of the resistant gene of blight, the identification method of the present invention does not utilize the conventional DNA linkage marking method, avoids the misjudgment caused by the loose DNA linkage, improves the accuracy of detection, and avoids unnecessary Production loss.

The invention provides a micro-Ribose Nucleic Acid (miRNA) for regulating and controlling the expression of a rustproofing bacterium infected gene of hybrid poplar and use of the miRNA. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar and the use of the miRNA have important biological significance and potential application value in poplar disease resistance breeding by virtue of study on the miRNA and the target gene thereof. The nucleotide sequence of the miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is as shown in SEQ ID NO: 1. The precursor sequence of the miRNA is as shown in SEQ ID NO: 2. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is capable of improving the resistance of the hybrid poplar to the rust disease and is applied to disease resistance breeding. The study on the miRNA by use of genetic engineering methods is capable of laying a foundation for intensively researching and controlling the rust disease infection resistance mechanism of the poplar and improving the resistance of the hybrid poplar to the rust disease.