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Identification method of rice bacterial leaf blight resistance and application of miRNA397a genetic locus

A bacterial blight resistance and gene locus technology, applied in the biological field, can solve problems such as inaccurate resistance to bacterial blight, achieve the effects of not easy degradation, improve accuracy, and avoid production loss

Active Publication Date: 2013-07-03
JIANGHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to solve the shortcomings of using DNA molecular markers to identify whether rice is resistant to bacterial blight is not accurate enough, the embodiment of the present invention provides an identification method for rice bacterial blight resistance and the application of the miRNA397a gene locus

Method used

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  • Identification method of rice bacterial leaf blight resistance and application of miRNA397a genetic locus

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Embodiment

[0026] The present invention mixes and hybridizes the miRNA gene of rice with the probe labeled miRNA397a gene, detects the result by non-denaturing polyacrylamide gel electrophoresis, compares the results of the rice to be tested and the susceptible rice, and then judges the To test whether rice is resistant to bacterial blight, the specific experimental process is as follows.

[0027] Susceptible rice: select non-bacterial blight-resistant rice seeds labeled 9311 with normal growth and no obvious pests and diseases on the surface.

[0028] Rice to be tested: select rice seeds with the label 9311 resistant to bacterial blight.

[0029] Among them, rice seeds resistant to bacterial blight have resistance gene Xa23.

[0030] Pre-treatment of seeds: Soak in 70% alcohol for 2 minutes, then wash twice with deionized water, soak in water at 30°C overnight, and germinate the seeds at a temperature of 30°C. After the buds grow out, sow In the seedling field, the management of the s...

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Abstract

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA397a genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA397a genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA397a gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA397a gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an identification method for rice bacterial blight resistance and the application of the miRNA397a gene locus. Background technique [0002] Rice is the most important food crop in my country. Bacterial blight is one of the two major diseases of rice. It is a worldwide disease, especially in Asian rice regions. The incidence of bacterial blight is high and the infection is fast, and the yield of affected paddy fields will be reduced by 20-30%, or even 50%. [0003] Breeding and utilizing disease-resistant varieties is the most economical and effective means to solve the grain yield reduction caused by bacterial blight. Therefore, it is necessary to identify the resistance of cultivated rice to bacterial blight. At present, the method for identifying rice bacterial blight usually uses DNA (Deoxyribonucleic acid, deoxyribonucleic acid) molecular markers, and uses the principle of DNA ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 彭海章伟雄张静周俊飞
Owner JIANGHAN UNIVERSITY
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