247results about How to "High killing efficiency" patented technology

A magnetic microphere of doxytaxol for preventing and treating tumor contains proportionaly magnetic Fe3O4 nanoparticles, alpha 1-acidic glucoprotein, doxytaxol and oleic acid. Its advantage is high target performance. Its preparing process is also disclosed.

The invention relates to the technical fields of molecular biology and cellular immunology and in particular relates to a preparation method of tumour cell specific polyclonal T cells. The preparation method of the tumour cell specific polyclonal T cells comprises the following steps: (1) obtaining peripheral blood mononuclear cells; (2) mixing the obtained peripheral blood mononuclear cells with gene modifying tumour cells; and (3) resuspending the obtained mixed cells in a culture medium containing cell factors which can maintain cell growth, proliferation and differentiation. The prepared tumour cell specific polyclonal T cells are mainly applied to medicines which are sued for preventing or treating cancers. The preparation method of the tumour cell specific polyclonal T cells has the advantages that the prepared tumour cell specific polyclonal T cells have the characteristics of large quantity, high specificity and strong killing capability; besides, a technology is simple, and the preparation cost is obviously reduced compared with the prior art.

The invention relates to a tumor specific killer cell preparation and a preparation method thereof. A specific killer cell is a mixture of DC (Dendritic Cell) vaccine, and CIK (Cytokine Induced Killer) and CTL (Cytotoxic Lymphocyte) cells. The preparation contains thymopentin; the preparation is mixed with the killer cells so as to have the effect of obviously improving the treatment effect.

The invention discloses an in-vitro large-scale amplification method of natural killer cells. The method comprises the following steps: collecting and separating peripheral blood mononuclear cells; sorting natural killer cells; culturing the natural killer cells; and collecting the natural killer cells. The method utilizing immunomagnetic beads to sort the natural killer cells has the advantages of simplicity in establishing and operation, and high comprehensive efficiency; and the effect of the method adopting in-vitro amplification of the immunomagnetic bead shorted NK cells is 2-3 times the effect of present amplification methods, so the method disclosed in the invention has great application values in the field of in-vitro large-scale amplification.

The invention provides an OCTS-CAR technique-based OCTS-CAR double-targeting chimeric antigen receptor, a coding gene and a recombinant expression vector and establishment and application of the OCTS-CAR double-targeting chimeric antigen receptor, the coding gene and the recombinant expression vector. The OCTS-CAR double-targeting chimeric antigen receptor includes a CD8 leader membrane receptor signal peptide, a double-antigen binding region, a CD8 Hinge chimeric receptor gemel, a CD8 transmembrane chimeric receptor transmembrane region, a CD28 chimeric receptor co-stimulatory factor, a CD134 chimeric receptor co-stimulatory factor and a TCR chimeric receptor T cell activating domain which are connected sequentially and in series, wherein the double-antigen binding region comprises heavy-chain VH and light-chain VL, connected in a certain mode, of two single-chain antibodies, an antibody Inner-Linker and an Inter-Linker between single-chain antibodies, and the two single-chain antibodies are formed by combining any two of a BCMA single-chain antibody, a CD319 single-chain antibody, a CD38 single-chain antibody, a PDL1 single-chain antibody and a CD123 single-chain antibody; in addition, the invention further provides a gene encoding the OCTS-CAR double-targeting chimeric antigen receptor, the recombinant expression vector and an establishment method and application of the gene encoding the OCTS-CAR double-targeting chimeric antigen receptor and the recombinant expression vector.

The invention relates to an antitumor cell immunotherapy technology, in particular to preparation of a specific tumor killing cell. The preparation method disclosed by the invention comprises the following steps of: 1, sampling a single prokaryotic cell from peripheral blood; 2, separating a DC (Dendritic Cell) from a T cell; 3, maturing the DC and preparing a DC vaccine; 4, preparing a CIK (Cytokine Induced Killer); 5, preparing a CTL (cytotoxic T lymphocyte); and 6, preparing a specific DC-CIK-CTL cell preparation.

The invention discloses a method for obtaining a tumor specific T cell receptor. The method comprises the following steps: firstly, obtaining a T cell receptor gene sequence in an antigen peptide specific T cell which comes from an immunoreactive positive and / or tumor symptom relieved tumor patient after antigen peptide treatment; then, preparing an antigen specific T cell receptor according to the antigen specific T cell receptor gene sequence; introducing the antigen peptide specific T cell receptor gene into the T cell, and expressing the antigen peptide specific T cell receptor gene to obtain the specific TCR-T cell. The specific TCR-T cell can be used for successfully killing gene mutation corresponding to antigen peptide and HLA typed tumor cell, and has high killing efficiency and agood application prospect.

The invention discloses a rotating mirror scanning dual waveband semiconductor laser sterilization system for a medical device, which comprises an infrared semiconductor laser, an ultraviolet semiconductor laser, optical fiber collimators, a zooming coupled lens assembly, an infrared beam scanning rotating mirror and an ultraviolet beam scanning rotating mirror. The system can be applied in various modes and occasions and can make important contribution to reduce a fatality rate and a disability rate and restore health as soon as possible in the field of medical treatment and intensive care infection prevention and treatment; the system achieves quick and thorough sterilization and has high efficiency by adopting an intense laser sterilization mode and integrating the advantages of a strong heating effect of an infrared laser and a strong protein molecular ionization damage effect of an ultraviolet laser; and the system is portable and reusable, is not contacted with a human body, has no obvious direct or indirect influence on the human body and is convenient to realize.

The invention discloses a computer virus searching and killing method based on the desktop cloud virtualization technology. The method mainly solves the problems that in the prior art, when virus killing is carried out on a virtualization platform, resources of a server are occupied, a virus storm and a virus killing storm are easily formed and management is inconvenient to achieve. When more than two independent virtual hosts are allocated for each physical host, one of the virtual hosts serves as an antivirus virtual machine, the rest of the virtual hosts serve as common virtual machines, a third-party antivirus software is integrated in the antivirus virtual machine so that the antivirus virtual machine can have capacity for searching and killing viruses and recording searching and killing results, antivirus agents are installed in the common virtual machines, and a computer virus characteristic value database is installed in the antivirus virtual machine. By the adoption of the scheme, the method is convenient to use and high in extendibility and has high practical value and promotional value.

The invention provides an allogeneic antigen-presenting cell targeting KRAS mutation, a construction method and a preparation method of intestinal cancer specific CTL cell, which belong to the technical field of tumor targeting, the method for constructing the allogeneic antigen-presenting cell comprises the following steps: 1) analyzing the affinity of a combination of HLA typing and KRAS mutation sequence of a certain intestinal cancer patient, and selecting a combination of synthetic fusion genes with the highest affinity; 2) ligating the synthetic fusion gene to an expression vector to obtain a recombinant vector; and 3) transferring the recombinant vector to a healthy-derived monocyte, and performing induced culture to obtain the allogeneic antigen-presenting cell targeting KRAS mutation. The allogeneic antigen-presenting cell targeting KRAS mutation solve the problem that the allogeneic DC does not match the HLA typing of the patient, and can stimulate a stronger immune response.The colon cancer-specific CTL cell has the good killing effect on target cells.

Provided, among other things, is a elastomeric medical glove having an antimicrobial surface coating comprising: a polymeric layer, and a dried, non-tacky coating on a surface of the polymeric layer, the coating comprising an antimicrobial agent, a wax agent, a lubricant, and a water-soluble wetting agent; wherein the glove comprises a residual powder of less than 2 mg per glove.

The invention discloses a lymphoblastic leukemia CAR-T (Chimeric Antigen Receptor-T Cell Immunotherapy) therapy carrier based on an OCTS (One CAR with Two SeFvs) technology. The lymphoblastic leukemia CAR-T therapy carrier comprises a lentivirus skeleton plasma, a human EF1alpha promoter, an OCTS chimeric receptor structural domain and an IL6R single-chain antibody, wherein the OCTS chimeric receptor structural domain comprises a CD8 leader chimeric receptor signal peptide and two groups of single-chain antibodies; the first group of single-chain antibodies is selected from any one of the following four groups of single-chain antibodies: a CD20 single-chain antibody light chain VL and a CD20 single-chain antibody heavy chain VH, a CD22 single-chain antibody light chain VL and a CD22 single-chain antibody heavy chain VH, a CD30 single-chain antibody light chain VL and a CD30 single-chain antibody heavy chain VH, and a CD123 single-chain antibody light chain VL and a CD123 single-chain antibody heavy chain VH; and the second group of the single-chain antibodies is a CD19 single-chain antibody light chain VL and a CD19 single-chain antibody heavy chain VH, an antibody Inner-Linker, a single-chain antibody Inter-Linker, a CD8-Hinge chimeric receptor linker, a CD8 Transmembrane chimeric receptor transmembrane zone, a TCR (T Cell Receptor) chimeric receptor T cell activation domain and a chimeric receptor co-stimulator zone. Besides, the invention discloses a constructing method for the carrier and application of the carrier to preparation of a drug for treating lymphoblastic leukemia.

The invention discloses an OCTS (One CAR (Chimeric Antigen Receptor) with two ScFvs (Single-chain variable Fragments)) technique based CAR-T (Chimeric Antigen Receptor-T cell immunotherapy) therapeutic vector for glioblastoma. The OCTS technique based CAR-T therapeutic vector comprises a lentiviral skeleton plasmid, a human EF1 [alpha] promoter (SEQ ID NO. 14), an OCTS chimeric receptor structural domain and a PDL1 single-chain antibody, wherein the OCTS chimeric receptor structural domain comprises a CD8 leader chimeric receptor signal peptide (SEQ ID NO. 15), a PDL1 single-chain antibody light chain VL (SEQ ID NO. 16), a PDL1 single-chain antibody heavy chain VH (SEQ ID NO. 17), an EGFRvIII single-chain antibody light chain VL (SEQ ID NO. 18), an EGFRvIII single-chain antibody heavy chain VH (SEQ ID NO. 19), an antibody Inner-Linker (SEQ ID NO. 20), a single-chain antibody Inter-Linker (SEQ ID NO. 21), a CD8 Hinge chimeric receptor linker (SEQ ID NO. 22), a CD8 Transmembrane chimeric receptor transmembrane domain (SEQ ID NO. 23), a TCR (T Cell Receptor) chimeric receptor T cell activation domain (SEQ ID NO. 26) and a chimeric receptor co-stimulator domain. In addition, the invention also discloses a construction method of the vector and application of the vector to the preparation of a medicine for treating the glioblastoma.

The invention relates to an immunomodulatory specific chimeric antigen receptor cell as well as a preparation method and application thereof. According to the invention, a recombinant vector of a chimeric antigen receptor of immune checkpoint regulation (PD1 or TIGIT) combined with specifically targeting human NKG2DL, and is used for modifying immune response cells; the obtained novel functionalized immune response cell can effectively target and attack various tumors, and a preparation for treating malignant tumors is prepared. Engineering immune cells modified by the chimeric antigen receptors of immune checkpoint regulation (PD1 or TIGIT) combined with specifically targeting human NKG2DL is adopted, inactivation of immune cells induced by inhibition factors is inhibited, immune escape of tumor cells is prevented, and antitumor activity can be obviously improved.

The invention provides a preparation method of a gold-copper sulfide nano material with a hollow core-shell structure and application thereof. The preparation method comprises the following steps of:mixing hexadecyl trimethyl ammonium bromide, chloroauric acid and sodium borohydride, and reacting to obtain gold seed solution; mixing the hexadecyl trimethyl ammonium bromide, silver nitrate, the chloroauric acid, ascorbic acid and the gold seed solution, and standing for growth to obtain a gold-core nano material; mixing polyvinylpyrrolidone, water, copper chloride and the gold-core nano material, and reacting to obtain a first reaction product; reacting the first reaction product with sodium hydroxide, reacting the obtained second reaction product with hydroxylamine hydrochloride, then reacting with sodium sulfide, centrifuging, cleaning obtained precipitates and then drying to obtain the gold-copper sulfide nano material with the hollow core-shell structure. The gold-copper sulfide nano material prepared by the preparation method has the advantages that under the irradiation of near-infrared light, the photothermal property of the copper sulfide is obviously improved by the plasmaresonance energy transfer mechanism of gold nano particles; the photodynamic property is higher; chemotherapeutic drugs also can be carried, and the tumor-cell killing efficiency is higher.

The invention relates to a common threewingnut root-containing Chinese medicinal composition for preventing and controlling parasites in aquaculture, a preparation method thereof and use thereof. The Chinese medicinal composition comprises the following components in percentage by weight: common threewingnut root 10-40, melia azedarach linn 10-30, lightyellow sophora root 10-40 and sessile stemona root 10-30. The Chinese composition of the invention contains a plurality kinds of parasite-killing active ingredients, is free from toxic and side effects and medicament residue, convenient in use, low in cost, high in parasite-killing efficiency and insusceptible of arousing medicament resistance and is an easily-degradable and environmentally-friendly Chinese medicinal composition for preventing and controlling diseases and parasites in aquaculture.

Bispecific antibodies directed to CD3 and CD20 and uses of such bispecific antibodies, in particular use thereof in the treatment of diseases in which specific targeting and T cell-mediated killing of cells that express CD20 is desired.

The invention discloses a mosquito killer which comprises a net tube, a plurality of layers of power grids, an air multiplied ring and a light source. The top of the net tube extends toward the inside to form a funnel-shaped opening. The power grids are mutually parallel, distributed along the axial direction of the net tube and fastened on the inner wall of the net tube. The air multiplied ring is sleeved and fastened on the outer wall of the net tube, and a wind outlet of the air multiplied ring is downward. The light source is arranged on the bottom inside the net tube. The mosquito killer is used so that mosquitoes nearby the killer are sucked inside the killer and can not escape. The mosquito killer has the advantages of being strong in mosquito tempting and high in killing efficiency.

The invention relates to a folic acid-nano-TiO2 composite photocatalyst. The folic acid-nano-TiO2 composite photocatalyst comprises TiO2 / SiO2 nanoparticles of which the surfaces are modified by folic acid and has a structural formula of FA-CONH-TiO2 / SiO2. Through amination coupling of a nano-TiO2 / SiO2 composite material and folic acid, compound stability and targeting properties are improved. Nano-TiO2 surfaces are coated with SiO2 so that nano-TiO2 photocatalytic efficiency is improved, nano-TiO2 thermostability is improved and agglomeration caused by nano-TiO2 surface properties is avoided. The folic acid-nano-TiO2 composite photocatalyst can be used for photocatalysis killing of cancer cells and has high killing efficiency and strong targeting properties.

The invention discloses a CAR-T (Chimeric antigen receptor T-cell immunotherapy) therapeutic vector for T lymphocytic leukemia. The therapeutic vector comprises a lentivirus skeleton plasmid, a humanEF1 alpha promoter and a CAR chimeric receptor domain, wherein the CAR chimeric receptor domain contains CD8 leader chimeric acceptor signal peptide, a CD7 single-chain antibody light chain VL, a CD7single-chain antibody heavy chain VH, an antibody inner hinge UCLinker, a CD8 Hinge chimeric receptor hinge, a CD8 Transmembrane chimeric receptor transmembrane region, a TCR chimeric receptor T cellactivation domain and a chimeric receptor co-stimulatory factor region. The invention further discloses a construction method of the vector and an application of the vector in preparation of a drug for treating T lymphocytic leukemia.

The invention discloses a marker used for in-vivo tracing and manual removal of CAR-T cells. The marker comprises a trace-linker 1 with a sequence as shown in SEQ ID No. 18, a trace-linker 2 with a sequence as shown in SEQ ID No. 19 and a trace-linker 3 with a sequence as shown in SEQ ID No. 19. The invention also discloses a CAR-T therapy vector including the marker and a construction method thereof. Moreover, the invention further discloses application of the marker to preparation of the CAR-T therapy vector and drugs used for treating triple-negative breast cancer. The marker provided by the invention can realize controllable in-vivo tracing, in-vitro separation and manual removal of CAR-T cells without influence on the tumor killing effect of the CAR-T cells, so the security of CAR-T cell therapy is greatly improved, and a powerful pool can be provided for deeper analysis of the process of CAR-T cell therapy. The vector provided by the invention can express a targeting chimeric antigen receptor of CD117 in human T lymphocytes, guides and activates killing effect of T lymphocytes on CD117 positive cells, and is applicable to treatment of triple-negative breast cancer in clinical practice.

The invention discloses a preparation method of a high-efficiency killer cell preparation adopting an immunodetection point dual-block CTL (cytotoxic lymphocyte). The preparation method comprises steps as follows: step 1), collecting mononuclear cells in peripheral blood; step 2), performing DC (dendritic cell) separation and T cell separation on the separated mononuclear cells with an adherence method; step 3), adding adhering DCs to a serum-free culture medium containing GM-CSF (granulocyte-macrophage colony-stimulating factor) and IL-4 (interleukin-4) for preparation of a DC vaccine; step 4), adding suspending T cells to CD3 and IL-2 for T cell culture and amplification; step 5), adding antigen-supported DCs to the amplified T cells for preparation of the CTL, and adding PD-1 (Programmed death 1) and CTLA-4 (cytotoxic t lymphocyte associated antigen-4) antibodies for preparation of the cell preparation with the dual-block CTL effect. According to the novel technology and the novel method provided by the invention, an immune brake effect is effectively regulated and T cell depletion is retarded by blocking inhibitory molecules on the tumor specific CTL surfaces, so that the tumor cytotoxicity of CTL effector cells is substantially improved.

The invention discloses a method and device for sterilizing a water body in combination with three-dimensional electrode and photocatalysis, and application of the method and the device. The method comprises the steps of: firstly, treating the water body to be treated by electrochemical reaction of the three-dimensional electrode; and then, sequentially treating the water body by photocatalytic reaction agent under the excitation of ultraviolet light to obtain the sterilized water body. The device for realizing the method comprises a three-dimensional electrode electrochemical sterilization pool and an ultraviolet light catalytic sterilization pool, which are sequentially connected with each other; the two pool walls of the three-dimensional electrode electrochemical sterilization pool are respectively provided with a cathode and an anode, which are connected with a power supply, and the three-dimensional electrode electrochemical sterilization pool is internally provided with three-dimensional electrode filler; and the pool wall of the ultraviolet light catalytic sterilization pool is fixedly provided with a photocatalyst layer, and the ultraviolet light catalytic sterilization pool is internally provided with a plurality of ultraviolet lamps used for exciting photocatalyst. After the technology for combining the three-dimensional electrode sterilization and ultraviolet lightcatalysis is adopted, the lower voltage and the ultraviolet light catalysis is carried out, waste water with higher bacteria-containing concentration can be treated efficiently, and the effect of continuous and efficient sterilization can be achieved by a one-step method, so that the bright and clear in application prospect is realized.

The present invention discloses a preparation method of a nano silver bactericide used for recirculated cooling water, and according to the method, chitosan and chitosan-based polymer are mainly used as carriers for immobilizing nanosilver. The preparation method comprises the following steps: (1) preparation of the immobilization carriers; (2) crosslinking activation of the immobilization carriers; (3) preparation of a nano-silver disinfecting solution; and (4) nano-silver particle immobilization for finally obtaining the immobilized nano silver bactericide. Compared with conventional bactericides, the nano silver bactericide has the characteristics of broad spectrum, high effect, no toxicity and strong antibacterial effect, and the like, is suitable for industrial recirculated cooling water systems, oil field injection water and wastewater treatment, has high kill efficiency on common heterotrophic bacteria, sulfate salt reducing bacteria and iron bacteria and other bacteria and algae, and has excellent effect of prevention of the formation of biofouling.

The invention relates to a medicine for treating and / or preventing cancer and an application. The medicine of the invention includes a recombinant MUC1-MBP fusion protein vaccine and a PD-1 antibody.The medicine is expected to break an immune tolerance state of a tumor microenvironment in vivo and enhance the MUC1 specific anti-tumor immune response, and is expected to completely clear tumors tobring gospel to the majority of cancer patients.

A high-energy laser device is composd of a high-power laser generator and electrode connected to high voltage. The laser beams generated by said laser generator pass through an high-voltage electric field generated by said electrode to ionize air and form a discharging channel. After the laser beams hit on a target, a discharge is triggered to release huge energy for destroy said target. Its advantage is high laser energy increased by 1000-1000000 times.

The invention belongs to the technical field of nano probes, and discloses a double-organelle-targeted nano probe as well as a preparation method and application thereof. The double-organelle-targetednano probe is mainly prepared from a compound of formula I and a photo-sensitive agent II; and the photo-sensitive agent II is phthalocyanines or porphyrins photo-sensitive agent with negative charges. The compound of formula I is a mitochondria-targeted chemotherapy reagent with an aggregation-induced luminescent effect. The nano probe of the invention not only can monitor the release process ina cell in real time by virtue of a double-fluorescent illumination way, but also can effectively improve the killing efficiency for cancer cells by virtue of the synergism of the mitochondria-targeted chemotherapy and lysosome-targeted photodynamics therapy. The invention also relates to application of nano probe in preparing an antitumor drug and / or fluorescent imaging. (The formulas are shown in the description).