195results about How to "Avoid purification process" patented technology

The invention relates to a preparation method of reduced graphene oxide, which comprises the following steps of: mixing reducing agent with water solution of graphene oxide in the absence of oxygen, and carrying out irradiation reduction reaction by high-energy ray irradiation to obtain reduced graphene oxide, wherein the reducing agent is alcohol with one to eight carbon atoms. The invention overcomes the problems on high temperature, toxicity, low efficiency, high energy consumption and the like in the current preparation method of reduced graphene oxide, and a novel, high-efficiency, economical and environment-friendly preparation method of reduced graphene oxide is provided. The method provided by the invention has no need to modify the reduced graphene oxide before grafting, has no need of any special reagent and is simple to operate, which is a method that has wide application range and in particular can realize large scale batch production; and the reduced graphene oxide prepared by the invention has remarkably improved conductivity and broader application field.

The invention relates to polymer grafted graphene oxide and a preparation method thereof. The method comprises the step that the polymer grafted modified graphene oxide is obtained through an irradiation graft polymerization reaction of C=C contained monomers and graphene oxide under anoxic conditions. The method of the invention, which does not need the modification of graphene oxide or polymers before grafting, has the advantages of simple operation process and wide application range, and is especially suitable for large-scale batch production; and the polymer grafted graphene oxide prepared in the invention has an obviously improved grafting rate, so the polymer grafted graphene oxide can be widely used.

The invention discloses a method for preparing gamma-valerolactone by acetylpropionic acid catalytic hydrogenation. Through high efficiency catalysis of levulinic acid hydrogenation based on a loaded ruthenium catalyst under mild conditions, gamma-valerolactone is prepared. The method has a levulinic acid conversion rate of 100% and gamma-valerolactone selectivity of 99.9%. The loaded ruthenium catalyst has a low active metal load capacity (less than 1.5w.t.%) and high activity (TOF, 7676h<1>), has good water and acid resistance and is suitable for an intermittent reactor and a continuous fixed bed reactor. The method solves the problem that the existing gamma-valerolactone preparation method needs a high temperature and high pressure and utilizes organic acids and bases and an organic solvent, improves preparation method economy and safety, utilizes a small amount of a catalyst, realizes catalyst recycle, has a high product yield and product separation easiness, and has a latent industrial application value.

The invention relates to the technical fields of molecular biology and cellular immunology and in particular relates to a preparation method of tumour cell specific polyclonal T cells. The preparation method of the tumour cell specific polyclonal T cells comprises the following steps: (1) obtaining peripheral blood mononuclear cells; (2) mixing the obtained peripheral blood mononuclear cells with gene modifying tumour cells; and (3) resuspending the obtained mixed cells in a culture medium containing cell factors which can maintain cell growth, proliferation and differentiation. The prepared tumour cell specific polyclonal T cells are mainly applied to medicines which are sued for preventing or treating cancers. The preparation method of the tumour cell specific polyclonal T cells has the advantages that the prepared tumour cell specific polyclonal T cells have the characteristics of large quantity, high specificity and strong killing capability; besides, a technology is simple, and the preparation cost is obviously reduced compared with the prior art.

The invention provides a method for preparing 5, 5'-bistetrazole-1, 1'-dioxo hydroxyl ammonium salt (TKX-50), and belongs to the technical field of organic synthesis. The method comprises the steps as follows: step one, glyoxime is prepared, and the yield is 62%; step two, a product obtained in the step one is dissolved in water and concentrated hydrochloric acid, chlorine is introduced at the temperature of 0 DEG C for a reaction for a period of time, and dichloroglyoxime is obtained; and finally, a product obtained in the step two is dissolved in a solvent, and the product and sodium azide have a reaction at the temperature of 0 DEG C for a period of time; after that, the mixture is transferred into diethy ether, and sealed for a reaction at the room temperature overnight after HCl is introduced for a period of time; and after diethyl ether and most HCl gas are volatilized, pH of an aqueous NaOH solution is regulated to be about 8, reflux cooling is performed, filtered and separated solids are dissolved in hydroxylamine hydrochloride to have a reaction for a period of time, and TKX-50 is obtained. According to the method, glyoxal is adopted as a raw material, water is adopted as a solvent for preparation of dichloroglyoxime in the step two, and only direct filtration is required in postprocessing, so that the tedious purification process is prevented, and the cost is reduced; and besides, a target product TKX-50 is synthetized through three steps of reactions, the total yield is up to 34%, the reaction condition is mild, the operation is simple and convenient, and the industrialization is easy to realize.

The invention provides a formula of a high temperature resistant hot melt adhesive and a processing process of the hot melt adhesive. The formula comprises the following components: polyamide, an ethylene-ethyl acrylate copolymer, an ethylene-vinyl acetate copolymer, a tackifier, an antioxidant and a modifier. The high temperature resistant hot melt adhesive is high in bonding strength, resistant to high temperature, excellent in insulating and sealing performance, resistant to a low temperature, environment-friendly, non-toxic, simple in production process and good in repeatability.

The invention discloses a refining method of 4-phenylaminopiperidine analgesic, comprising the following steps of firstly preparing an acid salt from the 4-phenylaminopiperidine analgesic, purifying the acid salt after recrystallization, alkalizing the salt again to obtain free basic groups, adding another acid to the mixture to obtain another acid salt, re-crystallizing and refining the obtainedanother acid salt by a second solvent to obtain the acid salt. According to the invention, the process is simple, the used agents are simply and easily obtained, and only twice salifying operation and recrystallization operation are needed; and by utilizing the refining method, the content of impurities is remarkably reduced, the purity reaches the weight standard associated with the pharmacopoeia, the purity and the yield of fentanyl compound acid salts prepared from fentanyl compound free basic groups are remarkably improved, the production cost is reduced, and the production efficiency is remarkably improved.

The invention provides a sophora davidii anthocyanin crude extract and further provides a microcapsule of the sophora davidii anthocyanin crude extract. The anthocyanin crude extract prepared by the invention has good antioxidant activity, so that further purification on anthocyanin is avoided, and the production and use cost is reduced. The invention provides the microcapsule, aiming at the problem that the anthocyanin as a main component is difficult to widely use in existing food because the anthocyanin is easy to oxidate and degrade under the action of illumination, high temperature and oxygen and cannot be dissolved in liposoluble substances, a microencapsulation technique which is used for keeping the stability of the anthocyanin and extending the application range thereof is provided, the tolerance for heat, light and the like is increased, and the stability is obviously increased.

The invention discloses a process of recycling terephthalic acid to produce DOTP (dioctyl terephthalate) by using polyester alkaline to reduce wastewater. The process comprises the following process steps: adding terephthalic acid reclaimed materials and isooctyl alcohol into a reaction kettle in a certain proportion, stirring and heating, adding a catalyst and activated carbon when water yield is obviously reduced, continuously stirring and heating the mixture, controlling the temperature of the mixture to 190-230 DEG C, carrying out dealcoholization in vacuum after reaction is ended, heating to 265-285 DEG C and distilling under reduced pressure under -0.09MPa to -0.1MPa to obtain raw ester, adding H2O2 in a certain proportion to discolor; and finally, absorbing impurities by use of activated carbon and kaolin and carrying out filter-pressing to obtain a nearly colorless DOTP finished product. According to the process, the raw materials can adopt untreated alkaline which is low in purity and chromaticity to reduce wastewater to recycle the terephthalic acid, so that a complex pretreatment process is avoided, the terephthalic acid reclaimed materials are utilized to the greatest extent, and the high ester content and the low colour and luster of the DOTP product are guaranteed while the efficiency is high and the energy consumption is low.

The invention provides a preparation method of a nanogold-fullerene nano composite material. The preparation method comprises the following steps of: preparing 4-aminobenzenethiol / n-hexanethiol protected nanogold, and connecting the nanogold with fullerene to prepare the nanogold-fullerene nano composite material. The connection comprises the following steps of: respectively dispersing the nanogold and the fullerene in a toluene or carbon tetrachloride solution, ultrasonically mixing the two solutions, and violently stirring at room temperature; removing a part of toluene or carbon tetrachloride by evaporation concentration; adding a methanol or acetone solution, and centrifuging; removing the supernatant, dissolving the precipitate in toluene, methylene dichloride or ethanol, and ultrasonically dispersing; and repeating the process of centrifuging, removing supernatant and resuspending until the ultraviolet absorption peak of the fullerene is eliminated in the supernatant, and centrifugally collecting to obtain the nanogold-fullerene nano composite material. The method does not relate to the complex chemical modification of fullerene, has mild reaction conditions and avoids a great many of complex chemical reactions and purification processes.

The invention provides a method for synthesizing Pro-Xylane through acylation protection and reduction. The method comprises the following steps: S1) mixing acetylacetone or acetoacetate compounds, xylose and alkaline substances in a solvent, and heating to react to obtain a first intermediate product; s2) carrying out acylation reaction on the first intermediate product and an acylation reagent to obtain a second intermediate product; and s3) carrying out reduction reaction on the second intermediate product and a reducing agent in a solvent to obtain the Pro-Xylane, wherein the reducing agent contains -1-valent hydrogen atoms. Compared with the prior art, the method has the advantages that three hydroxyls in the first intermediate product are acylated through acylation reaction to obtain the intermediate triester, the intermediate triester is easy to purify, and alpha-configuration products can be removed through extraction separation and recrystallization, so that purification in a column chromatography mode is avoided; and the intermediate triester is high in selectivity during reduction, and a strong reducing agent can be used for simultaneously reducing ketone carbonyl and ester groups to directly obtain high-selectivity (S)-Pro-Xylane.

The invention discloses a preparation method of solid phase peptide synthesis atosiban which includes the following steps: taking Rink Amide resins, Rink Amide MBHA resins or Rink Amide AM resins as starting materials and taking Fmoc amino acids as monomers, amino acids are grafted one by one and mercaptopropionic acids (Map(SX)) are protected by the last peptide chain; after protected nonapeptide resins are obtained, the acellular side-chain protective groups and cutting peptides are synchronized; then cutting peptides is carried out, and reduced crude atosiban is collected; the pH value is adjusted to 7.5 to 10.0, and oxidized crude atosiban is collected; target products are obtained by the separation and purification by preparative HPLC(C18 or C8 column). The preparation method is convenient in material source, simplifies technology and reduces cost; and the preparation method is low in the pollution of three wastes and is high in yield, and the preparation method is convenient for being industrialized and has good industrialization prospect.

The invention relates to a method for screening a polypeptide fragment with antifreeze activity. The method comprises a step of screening amino acid sequences of natural antifreeze protein, antifreeze protein or antifreeze glycoprotein or properly improving amino acid sequences of natural antifreeze protein, antifreeze protein or antifreeze glycoprotein. The invention further relates to antifreeze polypeptide, a bionic antifreeze surface related to the antifreeze polypeptide, a coating containing the antifreeze polypeptide and a preparation method and application of antifreeze polypeptide. The antifreeze polypeptide inherits antifreeze characteristics of the antifreeze protein, has a simple structure, is easy to synthesize on large scale and to produce in batches and exerts better effects compared with the antifreeze protein when used for preparation of the antifreeze surface.

The invention provides non-rusting silver alloy. The non-rusting silver alloy comprises, by weight, 90.0-92.5% of silver, 0.5-5% of copper, 0.5-2.0% of zinc, 0.2-1.1% of tin, 1.0-3.0% of indium and 0.1-6% of germanium. The invention further provides a preparation method for the non-rusting silver alloy and the application of the non-rusting silver alloy. According to the non-rusting silver alloy and the preparation method thereof, the compositions are simple and do not contain precious metal and rare metal; the non-rusting silver alloy is suitable for making contact with human body skin; good machinable property is achieved; the non-rusting property of the non-rusting silver alloy is over twice that of Sterling silver, so that the non-rusting silver alloy is suitable for being applied to the industries of jewelries, decorations and the like.

The invention provides a continuous production process of perfluor-2-methyl-3-pentanone. The method specifically comprises the step of carrying out contact with reactants successively by taking hexafluoropropylene as a raw material to obtain a product, wherein the contact means that different materials in a reactor coexist with a time shorter than or equal to 600s. The continuous production process provided by the invention has the advantages of being cheap and easily available in raw material, simple to operate, high in degree of automation, high in synthetic yield, few in byproduct, short inreaction time and the like.

The invention discloses a method for preparing macitentan. The method comprises the following steps: (1) carrying out a substitution reaction between a compound N-propanesulfonamide of a formula (II) and a compound 5-(4-bromophenyl)-4,6-dichloropyrimidine of a formula (III) so as to convert into a compound of a formula (IV); (2) carrying out a substitution reaction between the compound of the formula (IV) and a compound of a formula (V) in the presence of a strong base, thereby obtaining a compound of a formula (VI); and (3) carrying out a substitution reaction between the compound of the formula (VI) and a compound of a formula (VII) in the presence of a strong base, thereby obtaining the compound (macitentan) of the formula (I). The process conditions of the method for preparing macitentan are mild, the reaction time is greatly shortened, the after-treatment is simple, the purity is high, the reaction cost is low, and the industrial production is easily realized.

The invention provides a DNA amplification method. The DNA amplification method is characterized by comprising the step that a T7 promoter sequence is added to the target DNA tail end. According to the DNA amplification method, amplification can be conducted on DNA smaller than one nanogram or even a single cell genome DNA on the basis of trace DNA; relatively even amplification is achieved through the target DNA containing the T7 promoter sequence; an error produced in the amplification process can be effectively removed.

The invention provides a lycium ruthenicum anthocyanin crude extract and a controlled-release microcapsule thereof. The anthocyanin crude extract has good antioxidant activity, so that the further purification for an anthocyanin monomeric compound is prevented, and meanwhile, a preparation method is simple and convenient, production materials are easily available, and the production and use costs of the lycium ruthenicum anthocyanin are reduced. The controlled-release microcapsule is an eight-hour controlled-release capsule, the drug release is uniform in speed and complete, the bioavailability of the drug is improved, and the utilization ratio of the drug is guaranteed.

The invention provides a rivaroxaban purification method. The method comprises the following steps: dissolving a rivaroxaban coarse product in ethylene glycol monomethyl ether or n-butanol under a heating condition, adding active carbon after clarification, carrying out suction filtration when the solution is still hot, crystallizing and cooling the mother liquor, and filtering so as to obtain the crystallization product rivaroxaban. The rivaroxaban purification method has the following advantages: (1) ethylene glycol monomethyl ether or n-butanol is used to carry out recrystallization to avoid the complicated chromatogram purification; (2) compared to the acetate, ethylene glycol monomethyl ether or n-butanol will not corrode the reaction equipment and is convenient for industrial production.

The invention discloses a crosslinking type positively-charged chlorine-containing polymer filter membrane and a preparation method thereof. The filter membrane contains chlorine-containing polymers with tertiary amine side chains, or contains blends of chlorine-containing polymers with tertiary amine side chains and other chlorine-containing polymers. The preparation method of the crosslinking type positively-charged chlorine-containing polymer filter membrane mainly includes the following concrete steps that 1, tertiary amine monomers are grafted to chloride-containing copolymers through an in-situ atom transfer radical polymerization method; 2, a chlorine-containing polymer solution, obtained in the step 1, with tertiary amine side chains directly serves as a membrane forming solution, or other chlorine-containing polymers are added and stirred to form a uniform membrane forming solution, and a solid precursor membrane is formed through non-solvent inductive phase separation; 3, the solid precursor membrane is subjected to heat treatment to obtain the crosslinking type positively-charged chlorine-containing polymer filter membrane. The prepared crosslinking type positively-charged chlorine-containing polymer filter membrane has the advantages of being resistant to solvents, high in strength, controllable in bore diameter and retention performance, high in efficiency and low in cost, being positively charged and the like.

The invention relates to an irradiation-resistant hot melt adhesive, which comprises the following raw material ingredients by weight percentage: 10-40% of ethane-vinyl acetate copolymer, 20-50% of polyamide, 5-30% of irradiation-resistant rubber, 1-30% of adhesion agent, 1-10% of wax, 1-10% of stabilizing agent and 10% of plasticizer. The invention also provides a preparation method of irradiation-resistant hot melt adhesive. Compared with the present hot melt adhesive, the irradiation-resistant hot melt adhesive provided by the invention has the advantages of good caking property, low temperature-resistant performance and various oil products resistance. In addition, the preparation technology of the present invention is simple and stable and has good repeatability without harmful gas generation and harm to environment, low production energy consumption, and good economic effect and industrial production prospect.

This invention relates to a polyesteramide hot-melt adhesive for pyrocondensation material, and its preparation method from polyamide and water PET. The polyesteramide hot-melt adhesive has a repeating structure shown in this invention, wherein, R1 is residue derived from dimmer acid; R2 is -(CH2)2-12- and its possible branched isomer; R3 is terephthalate polyester segment (derived from polyester residue), terephthalate oligomer, terephthalate dimmer or benzene ring. The preparation method comprises ester-amide exchange, alcoholysis and polycondensation. By utilizing waste polyester as part of the the raw material, the product cost is reduced without sacrificing product quality, and meanwhile white pollution is reduced. The preparation method does not need solvent recovery or product purification, and has such advantages as no pollution, and simple and stable process.

The present invention provides a supported bifunctional catalyst which comprises a molecular sieve carrier, a component A and a component B which are supported on the molecular sieve carrier, and thechemical composition general formula of the catalyst is A-B / Beta, wherein the component A comprises a transition metal element which is a hydrogenation active center, the component B comprises an alkali metal element or an alkaline earth metal, and the molecular sieve carrier comprises a Beta molecular sieve. The invention also provides a preparation method and application of the supported bifunctional catalyst. The supported bifunctional catalyst can be used for preparing 2, 5-furandimethanol diethyl ether (BEMF) through one-step hydrogenation etherification of 5-hydroxymethylfurfural (HMF) catalyzed by the fixed bed, and has high hydrogenation activity and acid catalysis performance, and the HMF conversion rate and the target product yield can reach 99% or above and 75% or above respectively.

The invention discloses a measuring method for the algae biomass in a biological soil crust, and relates to the field of biology. The method includes the steps of sampling of an algae crust sample, sieving, cleaning, algae cultivating, chlorophyll a and chlorophyll b measuring, standard curve drawing and algae biomass measuring (natural development). In the algae cultivating step, cultivating is carried out through BG11 cultivating media, and use algae are mixed algae. In the chlorophyll a and chlorophyll b measuring step, chlorophyll is extracted with a mixed solution method, wherein extracting solutions comprise absolute ethyl alcohol, acetone and water in the ratio of 4.5 to 4.5 to 1. The relationship between the gross of the chlorophyll a and the chlorophyll b of the algae and the algae biomass is regressed through a standard curve. Measuring of the natural developing algae biomass is converted through the standard curve between the gross of the chlorophyll a and the chlorophyll b and the cultivated algae biomass. Direct comparison between the algae crust and the vascular plant biomass is achieved, and a reliable approach is provided for more accurately calculating the productivity of the algae crust and the effect of the productivity in an ecological system.

The invention discloses a type 1 bovine viral diarrhea virus (BVDV-1) virus-like particle as well as a preparation method and application thereof. A pFBD-BVDV-1 recombinant baculovirus transfer vectoris built by cloning structural protein C, Erns, E1 and E2 coding genes of BVDV-1 to pFastBacDual; a recombinant baculovirus vector Bac-BVDV-1 is obtained through the transposition in colibacillus DH10Bac; insect cells are transfected; recombinant baculovirus Baculo-BVDV-1 is obtained; the structural protein C, Erns, E1 and E2 of the insect cells for expressing BVDV-1 are infected and are automatically assembled into BVDV-1 virus-like particles (VLP) in the cells. The obtained virus-like particle is more similar to the morphostructure of the natural BVDV virus particles; after the inoculationinto animals, the bodies can be stimulated to generate good immune reaction; a better immune effect can be reached. The obtained virus-like particle can be directly purified by using the sucrose density gradient centrifugation. Compared with other expression systems, the virus-like particle has the advantages that the complicated process of extracellular assembly after the respectively purification of the structural protein is avoided; the efficiency is favorably improved; the cost is reduced; in addition, in the production process, the infective BVDV living viruses are not needed; the safetyis improved.

The invention provides a method for preparing nano gamma-AlOOH by utilizing a sodium metaaluminate solution and belongs to the field of inorganic nano-materials. The method comprises the following steps: 1) controlling the temperature of the sodium metaaluminate solution to be 15 to 30 DEG C; then adding a hydrogen peroxide solution into the sodium metaaluminate solution; after sufficiently reacting, collecting solid product in a reaction solution; 2) washing the solid product until the pH (Potential of Hydrogen) of a product suspension solution is 6.8 to 7.2; 3) filtering the product suspension solution in vacuum and drying a solid to prepare the nano gamma-AlOOH. The nano gamma-AlOOH prepared by the method is orthorhombic system AlOOH with the grain diameter of 70 to 200nm and is of a laminated structure; when a lot of the nano gamma-AlOOH is gathered, a plate sheet structure, which is distributed in layers, is formed.

The invention provides a method for preparing ethyl alcohol from acetate. The method includes the following steps: heating and vaporizing acetate, and under the condition of a catalyst and in a mixed gas atmosphere of CO and H2, introducing or not introducing inert gases to react for preparing the ethyl alcohol; the catalyst is Cu-M / SiO2, wherein Cu is used as an active component and accounts for 10-85 wt% of the total amount of the catalyst based on the calculation of copper oxides; M is used as an adjuvant, accounts for 0.1-20 wt% of the total amount of the catalyst based on the calculation of oxides of M, and refers to one or more of Mn, Zn, Fe, Co and Ni; the mass ratio of SiO2 in the catalyst is 10-89.9 wt%. The catalyst is used for preparing the ethyl alcohol by hydrogenation of acetate in the atmosphere of CO-containing H2 (syngas), CO and H2 in a feed gas have a synergistic effect with the catalyst, the activity of a hydrogenation reaction is improved, and the yield of the reaction product is increased significantly.

The invention provides a late transition metal catalyst for ethylene oligomerization and a preparation method thereof. The preparation method comprises the following steps: by using hyperbranched molecules using low generation terminal group as amido group and salicylaldehyde or salicylaldehyde derivatives as the raw materials in combination with anhydrous sodium sulfate and ethanol, carrying out reflux reaction under nitrogen conditions, and filtering to obtain a salicylaldehyde-Schiff base mixture; under nitrogen conditions, slowly and dropwisely adding a crystalline-water-bearing late transition metal halide ethanol solution into the salicylaldehyde-Schiff base mixture to react; and filtering and washing to obtain the late transition metal catalyst for ethylene oligomerization. The preparation method of the late transition metal catalyst for ethylene oligomerization prepared by the one-pot process is simple, and avoids the complex process of purifying the ligand in the conventional late transition metal catalyst for ethylene oligomerization; and the catalyst yield is 80-90%. The catalyst has higher catalytic ethylene oligomerization activity, and can easily implement industrial production.