77results about How to "High peptide yield" patented technology

This invention discloses a preparation method of solid-phase peptide synthesizing bivalirudin. It includes the following steps: taking any one of triphenyl methyl chloride resin, 4-methyl-triphenyl methyl chloride resin, 4-methoxy-triphenyl methyl chloride resin, 2-chlorine-triphenyl methyl chloride resin, or Wang resin as the starting raw materials, connecting amino acids in turn according to the method of solid-phase synthesis, to get a protective 28-peptide resin, removing Fmoc-protective group in turn, side-chain protecting group and cutting the peptide to get a crude, then purifying the crude through C18 (or C8) high-pressure column to get bivalirudin exquisite article. In this invention, the peptide yield of every step is more than 99%, and the total yield is 14%.

The invention relates to a process for preparing corn peptides by taking corn starch sugar residues as raw materials. The process comprises the following steps of: crushing corn starch sugar residues, adding absolute ethyl alcohol to extract a yellow pigment, drying the materials subjected to complete pigment extraction, crushing and sieving, adding water, adjusting the pH value, and respectively adding alkaline protease and flavourzyme to hydrolyze; adjusting the pH value by using hydrochloric acid and adding active carbon to decolor and remove bitter; desalting the obtained feed liquid by using a membrane system, and freezing and drying in vacuum to prepare corn peptides powder. According to the process, the corn starch sugar residues are used as the materials to prepare the corn peptides, and the process is low in cost, material consumption and energy consumption, and is beneficial to the improvement of resource utilization of by-products.

The invention provides a preparation method of soybean whey polypeptides with liver protection and antioxidation effects. The preparation method comprises the following steps: carrying out flocculation treatment and impurity removal on soybean whey wastewater, adding 0.5-1.5 grams of cysteine to each liter of whey water and carry out hydrolysis for 5-7 hours under the conditions that 1000-2500 activity unit of acid protease is added to each gram of proteins, the pH value is 2-3 and the temperature is 40-55 DEG C; and carrying out hydrolysis for 3-5 hours under the conditions that 1000-2500U / g of pepsase is added, the temperature is 35-45 DEG C and pH value is 2-3; and carrying out enzyme killing, centrifugation, filtration, concentration and drying on hydrolysate to obtain the soybean whey polypeptides. Animal experiments prove that the soybean whey polypeptides have strong antioxidation and acute liver injury resistance effects. The polypeptides contain more than 80% of small molecular oligopeptides below 1000Da. Through human test, the polypeptides have good taste and do not have side effects. The polypeptides can serve as the raw materials of common nutritional and health food.

The invention relates to preparation of octreotide acetate and an octreotide acetate injection pharmaceutical composition. Specifically, the invention relates to a method for preparing the octreotide acetate; the method comprises the following steps: taking merrifield resin as a starting raw material and preparing Boc-Thr(tBu)-OH into cesium salt; sequentially connecting amino acid with protecting groups according to a solid-phase synthesis method to obtain protected octapeptide resin; removing Boc-protecting groups in sequence by utilizing HCl / isopropyl alcohol and carrying out peptide linking reaction by utilizing a condensing agent; reducing with palladium-carbon / hydrogen; meanwhile, chopping off a peptide chain to obtain reduced octreotide; ventilating air under the condition that the pH (Potential of Hydrogen) is 8 to 9 to form a ring by a disulfide bond, so as to obtain an octreotide crude product; separating and purifying the octreotide crude product through a C18 column to prepare refined octreotide. The invention further relates to the octreotide acetate injection pharmaceutical composition. The method for preparing the octreotide acetate and the octreotide acetate injection pharmaceutical composition, provided by the invention, have the excellent technical effects shown in the description.

A method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles is characterized by comprising the steps: drying cordyceps militaris at the temperature of 60-80 DEG C, carrying out coarse crushing, passing through a 40-mesh sieve, then carrying out nano crushing for 3-5 h, and thus obtaining cordyceps militaris nanoparticles; adding the cordyceps militaris nanoparticles to distilled water, shaking evenly, adjusting to a natural pH value, sterilizing for 20-30 min in a high-pressure sterilization pot at the temperature of 121 DEG C, and thus obtaining a seed culture medium; putting a beef extract, peptone and sodium chloride in distilled water, allowing the pH to be 6-7, carrying out high-pressure steam sterilization for 20-30 min at the temperature of 121 DEG C, cooling to obtain an ordinary beef extract-peptone culture medium, inoculating the ordinary beef extract-peptone culture medium with a slant strain of a bacillus licheniformis strain, at the temperature of 30-40 DEG C, culturing for 1-2 d, to obtain inoculated and activated bacillus licheniformis, inoculating the seed culture medium with the activated bacillus licheniformis, and thus obtaining the antioxidative peptide product. The method is high in efficiency, low in cost, short in production cycle, and suitable for large-scale production.

The invention relates to a bioactivator preparation method, in particular to a placenta polypeptide preparation method. The placenta polypeptide preparation method comprises the steps of cutting a placenta into pieces, and adding normal saline into the cut placenta for tissue homogenate; mixing the obtained product with protease for enzymatic hydrolysis, and performing centrifugation and collecting centrifugate; performing extraction through reverse micelles to produce placental polypeptides and crude protease extract, subjecting the crude protease extract to ultrafiltration, collecting ultrafiltrate, and drying the ultrafiltrate to produce the placenta polypeptides. The preparation method is simple, production cost is low, equipment investment is little, and large-scale production is facilitated.

The invention discloses a Telis-vasophysin preparing method of solid-phase polypeptide, which comprises the following steps: adopting Rink Amide resin (concluding Rink Amide MBHA resin, Rink AmideAM resin) as original material, Fmoc protective amino acid as monomer, TBTU or HBTU-to-HOBt as condensing agent to connect amino sequently; using Boc-Gly-OH for the last peptide chain; adding peptide cutting agent to cut peptide; adding ether to deposit to obtain crude product; adding alkali material to oxidate at 7.5-10.0 pH value to generate oxide crude product; proceeding separation and purifying through C18 (or C8) pillar to produce object product. The method possesses low manufacturing cost, simple technology, high obtaining rate and low environmental pollution, which is convenient to do industrial construction.

The invention discloses an industrial production method for enzymatic preparing wheat peptide from wheat gluten. The production method is characterized by comprising the following steps: (1) putting wheat gluten of 1kg, adding deionized water, and reflowing to a pot; (2) heating up to 50 to 55 DEG C, pH of feed fluid is 7.5 to 8.0, then adding protamex of 1 to 2g, and carrying out enzymolysis for8 hours; (3) heating up to 80 DEG C, and extinguishing enzyme for 10min; and (4) carrying out filtration by a ceramic membrane and vacuum concentration, treating the feed fluid with spray drying, sieving by a sieve with 60 meshes, and packing by compound bags. The wheat peptide prepared by the method of the invention has the protein content of above 50 percent, molecular weight of wheat peptide of 90 percent is below 1000Dalton. The method of the invention has simple technique and short production cycle, the wheat peptide product produced by the method is white and has good mouthfeel.

The invention discloses a bright-ala-ruilin preparing method of solid-phase polypeptide, which comprises the following steps: adopting Wang resin or CTC resin as original material to connect amino with protective group to produce protective nonapeptide resin; removing Fmoc-protective group sequently; proceeding side-chain protective group synchronizingly and cutting peptide; connecting ethylaminethrough ethylamine-to-HOBT to produce crude product; proceeding separation and purifying through C18 (or C8) pillar to produce fine bright-ala-ruilin. The invention avoids the utility of poisonous agent, which improves the purifying, peptide connecting and obtaining rate.